Construct: ORF TRCN0000478902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009844.1_s317c1
- Derived from:
- ccsbBroadEn_07242
- DNA Barcode:
- TTCATTGCTAGTTGATATCTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CUL4B (8450)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8450 | CUL4B | cullin 4B | NM_003588.3 | 99.9% | 99.8% | 2179C>A |
| 2 | human | 8450 | CUL4B | cullin 4B | NM_001330624.1 | 97.9% | 97.4% | (many diffs) |
| 3 | human | 8450 | CUL4B | cullin 4B | NM_001079872.1 | 97.8% | 97.4% | (many diffs) |
| 4 | human | 8450 | CUL4B | cullin 4B | NM_001369145.1 | 78.2% | 77.7% | (many diffs) |
| 5 | mouse | 72584 | Cul4b | cullin 4B | NM_001110142.1 | 85.1% | 89.7% | (many diffs) |
| 6 | mouse | 72584 | Cul4b | cullin 4B | NM_028288.5 | 85.1% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2805
- ORF length:
- 2739
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gtcacagtca tctggatcag gagatgggaa tgatgatgag gctactacct 121 ctaaagacgg tggtttttct tcccccagtc cctcagctgc tgctgctgct caggaggtca 181 gatctgccac tgatggtaat accagcacca ctccgcccac ctctgccaag aagagaaagt 241 taaacagcag cagcagtagc agcagtaaca gtagtaacga gagagaagac tttgattcca 301 cctcttcctc ctcttccact cctcctttac aacccaggga ttcggcatcc ccttcaacct 361 cgtccttctg cctgggggtt tcagtggctg cttccagcca cgtaccgata cagaagaagc 421 tgcgttttga agacaccctg gagtttgtag ggtttgatgc gaagatggct gaggaatcct 481 cctcctcctc ctcctcatct tcaccaactg ctgcaacatc tcagcagcag caacttaaaa 541 ataagagtat attaatctct tctgtggctt cggtgcatca tgcaaacggc ctagccaaat 601 cttctaccac cgtctctagc tttgctaaca gcaaacctgg ctctgctaag aagttagtga 661 tcaagaactt taaagataag cctaaattac cagaaaacta cacagatgaa acctggcaaa 721 aactgaaaga agcagtggaa gctattcaga atagtacttc aattaagtac aatttagaag 781 aactctacca ggctgtagaa aatctctgtt cttacaagat ttctgcaaac ttgtacaaac 841 agctgagaca gatctgcgaa gatcacatca aagcacagat tcatcaattc agagaggatt 901 cattggatag cgttcttttt ttaaagaaga ttgatagatg ctggcaaaac cattgcagac 961 aaatgatcat gatcaggagc atttttttgt ttctggatag aacttacgtt cttcagaatt 1021 caatgctacc ctccatttgg gacatgggac tggagttatt tagggctcat attataagtg 1081 atcagaaagt gcagaataag acaattgatg gcattcttct cttgattgag agggaaagga 1141 atggtgaagc aattgataga agtttacttc gaagcctttt aagcatgctg tctgatttgc 1201 aaatttatca agattctttt gaacaacgat ttttggaaga aactaaccgg ctctatgcag 1261 ctgaaggcca aaaattaatg caagaaagag aggttcctga atatctacat catgttaaca 1321 aacgtctaga agaagaagca gacagactta ttacttactt agatcagacc acccagaagt 1381 cattaattgc tactgtagaa aaacaacttc taggtgaaca cttaacagca attcttcaga 1441 aaggtttaaa taacctcctt gatgaaaacc gaattcaaga tttgtctctt ctgtatcagc 1501 tcttcagtag agttcgaggt ggagttcagg ttcttttgca gcagtggatc gaatatatca 1561 aggcatttgg cagcactatt gtaattaatc ctgaaaaaga taaaaccatg gttcaagaat 1621 tgctggattt taaagataag gttgaccata taattgatat ctgctttctg aagaatgaga 1681 aatttatcaa tgccatgaaa gaagcatttg aaacgttcat taacaaaaga ccaaataaac 1741 cagctgaact tatagctaag tatgtagatt caaaacttcg tgcaggcaac aaagaagcta 1801 cagatgaaga acttgagaaa atgttggata aaattatgat catatttaga tttatctatg 1861 gcaaggatgt ttttgaggcc ttctataaga aagatttagc caagcgcctg ttagtcggaa 1921 agagtgcatc tgtagatgct gaaaaatcaa tgctgtccaa acttaaacat gaatgcggag 1981 ctgctttcac cagcaaactt gaaggaatgt ttaaagacat ggaactttct aaagacatca 2041 tgattcagtt caaacagtat atgcagaatc agaatgttcc gggaaatatt gagttaactg 2101 tgaatatcct gacaatgggc tattggccga catatgtgcc tatggaagtt catttaccac 2161 cagagatggt aaaacttcag gagattttca agacatttta cctaggcaaa catagtggca 2221 ggaaacttca gtggcagtca accataggac actgtgtgtt aaaagcagaa tttaaagagg 2281 gtaaaaagga actccaggtc tctctttttc aaacactggt gctgctaatg tttaatgagg 2341 gagaggagtt cagtttagaa gagatcaagc aggcaactgg aatagaggat ggagagttaa 2401 ggagaacact gcagtcatta gcctgtggca aagctagagt tctggcgaaa aatccaaagG 2461 GCAAAGACAT TGAAGATGGT GACAAGTTCA TTTGTAATGA TGATTTCAAA CATAAACTTT 2521 TCAGGATAAA GATCAATCAA ATCCAGATGA AAGAAACGGT TGAAGAACAA GCAAGCACTA 2581 CAGAAAGAGT ATTTCAAGAC AGACAGTATC AAATTGATGC TGCAATTGTT CGAATTATGA 2641 AGATGAGAAA GACACTTAGC CACAATCTCC TTGTTTCAGA AGTGTACAAC CAGTTGAAAT 2701 TTCCAGTAAA GCCTGCTGAT CTTAAGAAGA GAATAGAATC TTTAATTGAC CGGGACTACA 2761 TGGAAAGAGA TAAAGAAAAT CCAAACCAGT ACAACTATAT TGCATACCCA ACTTTCTTGT 2821 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2881 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2941 GAAAGGACGA TTCATTGCTA GTTGATATCT TTTCACGCGT TAAGTCgaca atcaacctct 3001 ggattacaaa atttgtgaaa gatt