Construct: ORF TRCN0000478924

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004023.2_s317c1
Derived from:
ccsbBroadEn_02516
DNA Barcode:
CAATCAGTGATTTGGTCCAAAAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STAG2 (10735)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478924
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10735 STAG2 stromal antigen 2 NM_001042751.2 100% 100%
2 human 10735 STAG2 stromal antigen 2 NM_001282418.2 100% 100%
3 human 10735 STAG2 stromal antigen 2 NM_006603.5 100% 100%
4 human 10735 STAG2 stromal antigen 2 XM_005262361.2 100% 100%
5 human 10735 STAG2 stromal antigen 2 XM_017029232.1 100% 100%
6 human 10735 STAG2 stromal antigen 2 XM_017029233.1 100% 100%
7 human 10735 STAG2 stromal antigen 2 XM_017029234.1 100% 100%
8 human 10735 STAG2 stromal antigen 2 XM_024452328.1 100% 100%
9 human 10735 STAG2 stromal antigen 2 XM_024452329.1 100% 100%
10 human 10735 STAG2 stromal antigen 2 NM_001042749.2 97% 97% 3468_3578del
11 human 10735 STAG2 stromal antigen 2 NM_001042750.2 97% 97% 3468_3578del
12 human 10735 STAG2 stromal antigen 2 XM_005262357.3 97% 97% 3468_3578del
13 human 10735 STAG2 stromal antigen 2 XM_005262358.2 97% 97% 3468_3578del
14 human 10735 STAG2 stromal antigen 2 XM_005262359.4 97% 97% 3468_3578del
15 human 10735 STAG2 stromal antigen 2 XM_005262360.2 97% 97% 3468_3578del
16 human 10735 STAG2 stromal antigen 2 XM_006724727.1 97% 97% 3468_3578del
17 human 10735 STAG2 stromal antigen 2 XM_011531253.2 97% 97% 3468_3578del
18 human 10735 STAG2 stromal antigen 2 XM_024452326.1 97% 97% 3468_3578del
19 human 10735 STAG2 stromal antigen 2 XM_024452327.1 97% 97% 3468_3578del
20 human 10735 STAG2 stromal antigen 2 XM_006724728.4 91.6% 91.6% 0_1ins207;3261_3371del
21 human 10735 STAG2 stromal antigen 2 XM_017029235.1 91.6% 91.6% 0_1ins207;3261_3371del
22 mouse 20843 Stag2 stromal antigen 2 NM_001077712.2 93.9% 99.1% (many diffs)
23 mouse 20843 Stag2 stromal antigen 2 NM_021465.4 93.9% 99.1% (many diffs)
24 mouse 20843 Stag2 stromal antigen 2 XM_011250989.2 93.9% 99.1% (many diffs)
25 mouse 20843 Stag2 stromal antigen 2 XM_017318446.1 93.9% 99.1% (many diffs)
26 mouse 20843 Stag2 stromal antigen 2 NM_001290713.1 91.1% 96.2% (many diffs)
27 mouse 20843 Stag2 stromal antigen 2 XM_011250983.2 91.1% 96.2% (many diffs)
28 mouse 20843 Stag2 stromal antigen 2 XM_011250984.2 91.1% 96.2% (many diffs)
29 mouse 20843 Stag2 stromal antigen 2 XM_011250985.2 91.1% 96.2% (many diffs)
30 mouse 20843 Stag2 stromal antigen 2 XM_011250986.2 91.1% 96.2% (many diffs)
31 mouse 20843 Stag2 stromal antigen 2 XM_011250987.1 91.1% 96.2% (many diffs)
32 mouse 20843 Stag2 stromal antigen 2 XM_011250988.1 91.1% 96.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3759
ORF length:
3693
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat agcagctcca gaaataccaa ctgattttaa tctactacag gagtcagaaa
121cacatttttc ttctgacaca gattttgaag atatcgaagg aaaaaaccaa aagcaaggca
181aaggcaaaac ttgtaaaaaa ggcaaaaagg gcccagcaga aaagggcaaa ggtggaaatg
241gaggaggaaa acctccttct ggtccaaacc gaatgaatgg tcatcaccaa cagaatggag
301tggaaaacat gatgttgttt gaagttgtta aaatgggcaa gagtgctatg cagtcggtgg
361tagatgattg gatagaatca tacaagcatg accgagatat agcacttctt gaccttatca
421acttttttat tcagtgttca ggctgtaaag gagttgtcac agcagaaatg tttagacata
481tgcagaactc tgagataatt cgaaaaatga ctgaagaatt cgatgaggat agtggagatt
541atccacttac catggctggt cctcagtgga agaagttcaa atccagtttt tgtgaattca
601ttggcgtgtt agtacggcaa tgtcaatata gtatcatata tgatgagtat atgatggata
661cagtcatttc acttcttaca ggattgtctg actcacaagt cagagcattt cgacatacaa
721gcaccctggc agctatgaag ttgatgacag ctttggtgaa tgtggcacta aatcttagca
781ttaatatgga taatacacaa agacaatatg aagcagaacg gaataaaatg attggaaaac
841gagccaatga gaggctagaa ctcctgctac aaaagcggaa agagcttcag gaaaatcaag
901atgaaataga aaatatgatg aatgcaatat ttaaaggagt gtttgtacat agataccgtg
961atgcgatagc tgaaattcga gctatttgca ttgaagagat tggcatttgg atgaagatgt
1021atagtgatgc ctttcttaat gacagttatt taaaatatgt tggttggact atgcatgata
1081agcaaggtga agtaagactc aaatgtctta ctgctctaca agggctttat tataacaaag
1141agcttaattc caaactggaa ctttttacca gtcggttcaa ggatagaatt gtgtctatga
1201cccttgacaa agaatatgat gttgcagtac aagcaataaa attactcact cttgttttac
1261agagtagtga agaagttctc actgcagaag attgtgaaaa tgtctatcat ctggtttatt
1321cagctcaccg gccagtagca gtagcagctg gagaatttct ctacaaaaag ctcttcagtc
1381gtagagatcc agaggaggat ggaatgatga aaagaagagg aagacaaggt ccaaatgcca
1441accttgttaa gacattggtt tttttctttc tagaaagtga gttacatgag catgcagcat
1501accttgtgga tagcatgtgg gactgtgcta ctgagctgct gaaagactgg gaatgtatga
1561atagcttgtt actggaagag ccacttagtg gagaggaagc actaacagat aggcaagaga
1621gtgctctgat tgaaataatg ctttgtacca ttagacaagc ggctgaatgt catcctcccg
1681tgggaagagg gacaggaaaa agggtgctta cagcaaagga gaagaagaca cagttggatg
1741ataggacaaa aatcactgag ctttttgccg tggcccttcc tcagttatta gcaaaatact
1801ctgtagatgc agaaaaggtg actaacttgt tgcagttgcc tcagtacttt gatttggaaa
1861tatataccac tggacgatta gaaaagcatt tggatgcctt attgcgacag atccggaata
1921ttgtagagaa gcacacagat acagatgttt tggaagcatg ttctaaaact taccatgcac
1981tctgtaatga agagttcaca atcttcaaca gagtagatat ttcaagaagt caactgatag
2041atgaattggc agataaattt aaccggcttc ttgaagattt tctgcaagag ggtgaagaac
2101ctgatgaaga tgatgcatat caggtattgt caacattgaa gaggatcact gcttttcata
2161atgcccatga cctttcaaag tgggatttat ttgcttgtaa ttacaaactc ttgaaaactg
2221gaatcgaaaa tggagacatg cctgagcaga ttgttattca cgcactgcag tgtactcact
2281atgtaatcct ttggcaactt gctaagataa ctgaaagcag ctctacaaag gaggacttgc
2341tgcgtttaaa gaaacaaatg agagtatttt gtcagatatg tcaacattac ctgaccaacg
2401tgaatactac tgttaaggaa caggccttca ctattctgtg tgatattttg atgatcttca
2461gccatcagat tatgtcagga gggcgtgaca tgttagagcc attagtgtat acccctgatt
2521cttcattgca gtctgagttg ctcagcttta ttttggatca tgtcttcatt gaacaggatg
2581atgataataa tagtgcagat ggtcagcaag aggatgaagc cagtaaaatt gaagctctgc
2641acaagagaag aaatttactt gcagcatttt gtaagctaat tgtatatact gtggtggaga
2701tgaatacagc tgcagatatc ttcaaacagt atatgaagta ttataatgac tatggagata
2761tcatcaaaga aacaatgagt aaaacaaggc agatagacaa aattcagtgt gctaagaccc
2821ttattctcag tctgcaacag ctttttaatg aaatgataca agaaaatggc tataattttg
2881atagatcatc ctctacattt agtggcataa aagaacttgc tcgacgtttt gctttaactt
2941ttggacttga tcagttgaaa acaagagaag ccattgccat gctacacaaa gatggcatag
3001aatttgcttt taaagagcct aatccgcaag gggagagcca tccaccttta aatttggcat
3061ttcttgatat tctgagtgaa ttttcttcta aactacttcg acaagacaaa agaacagtgt
3121atgtttactt ggaaaagttc atgacctttc agatgtcact ccgaagagag gatgtgtggc
3181ttccactgat gtcttaccga aattctttgc tagctggtgg tgatgatgac accatgtcag
3241tcattagtgg aatcagcagc cgggggtcaa cagtacggag taaaaaatca aaaccatcta
3301caggaaaacg gaaagtggtt gagggcatgc agctttcact cactgaagaa agtagtagta
3361gtgacagtat gtggttaagc agagaacaaa CACTGCACAC CCCTGTTATG ATGCAGACAC
3421CACAACTCAC CTCCACTATT ATGAGAGAGC CCAAAAGATT ACGGCCTGAG GATAGCTTCA
3481TGAGTGTTTA TCCAATGCAG ACTGAACATC ATCAAACACC TCTTGATTAT AATCGGCGTG
3541GCACAAGCCT AATGGAAGAT GATGAAGAGC CAATTGTGGA AGATGTTATG ATGTCCTCAG
3601AAGGGAGGAT TGAGGATCTT AATGAGGGAA TGGATTTTGA CACCATGGAT ATAGATTTGC
3661CACCATCAAA GAACAGACGA GAGAGAACAG AACTGAAGCC TGATTTCTTT GATCCAGCTT
3721CAATTATGGA TGAATCAGTT CTTGGAGTGT CAATGTTTTA CCCAACTTTC TTGTACAAAG
3781TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
3841GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
3901ACGACAATCA GTGATTTGGT CCAAAAAAAC GCGTTAAGTC gacaatcaac ctctggatta
3961caaaatttgt gaaagatt

Download FASTA (ORF) (Full)