Transcript: Mouse XM_011250989.2

PREDICTED: Mus musculus stromal antigen 2 (Stag2), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Stag2 (20843)
Length:
6135
CDS:
545..4240

Additional Resources:

NCBI RefSeq record:
XM_011250989.2
NBCI Gene record:
Stag2 (20843)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011250989.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000295339 ATTGCGCCAGATCCGAAATAT pLKO_005 2380 CDS 100% 15.000 21.000 N Stag2 n/a
2 TRCN0000108975 CGACCCTGAAATTGTAGGATA pLKO.1 4670 3UTR 100% 4.950 6.930 N Stag2 n/a
3 TRCN0000295340 TACGAGGCAGAACGGAATAAA pLKO_005 1286 CDS 100% 15.000 12.000 N Stag2 n/a
4 TRCN0000295274 GATAAGAATCTTCTCACATTT pLKO_005 4563 3UTR 100% 13.200 10.560 N Stag2 n/a
5 TRCN0000108976 GCGCTTCTTGACCTTATCAAT pLKO.1 881 CDS 100% 5.625 4.500 N Stag2 n/a
6 TRCN0000287988 GCGCTTCTTGACCTTATCAAT pLKO_005 881 CDS 100% 5.625 4.500 N Stag2 n/a
7 TRCN0000152523 GCAAGCAGTCTTCAGGTTAAA pLKO.1 5316 3UTR 100% 13.200 9.240 N STAG2 n/a
8 TRCN0000342744 GCAAGCAGTCTTCAGGTTAAA pLKO_005 5316 3UTR 100% 13.200 9.240 N STAG2 n/a
9 TRCN0000108977 CCTCAACATTTAGTGGCATAA pLKO.1 3369 CDS 100% 10.800 7.560 N Stag2 n/a
10 TRCN0000108979 CCTGATGAAGATGACGCATAT pLKO.1 2579 CDS 100% 10.800 7.560 N Stag2 n/a
11 TRCN0000287989 CCTGATGAAGATGACGCATAT pLKO_005 2579 CDS 100% 10.800 7.560 N Stag2 n/a
12 TRCN0000151938 CCAACGTGAATACTACTGTTA pLKO.1 2874 CDS 100% 4.950 3.465 N STAG2 n/a
13 TRCN0000108978 CCGATTTCTTTGATCCAGCTT pLKO.1 4179 CDS 100% 2.640 1.848 N Stag2 n/a
14 TRCN0000153201 CCTCTGCTGGATTTCTGTTTA pLKO.1 4612 3UTR 100% 13.200 7.920 N STAG2 n/a
15 TRCN0000152009 CACTATGTAATCCTTTGGCAA pLKO.1 2756 CDS 100% 2.640 1.848 N STAG2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011250989.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02516 pDONR223 100% 93.9% 99.1% None (many diffs) n/a
2 ccsbBroad304_02516 pLX_304 0% 93.9% 99.1% V5 (many diffs) n/a
3 TRCN0000478924 CAATCAGTGATTTGGTCCAAAAAA pLX_317 10.2% 93.9% 99.1% V5 (many diffs) n/a
Download CSV