Construct: ORF TRCN0000478926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015855.1_s317c1
- Derived from:
- ccsbBroadEn_09586
- DNA Barcode:
- CAGCCATCTTTGTGTGCCTTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB6 (140459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478926
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140459 | ASB6 | ankyrin repeat and SOCS box... | NM_017873.4 | 99.8% | 100% | 207G>C;855C>T |
| 2 | human | 140459 | ASB6 | ankyrin repeat and SOCS box... | NM_001202403.1 | 92.9% | 93.1% | 207G>C;510_511ins87;768C>T |
| 3 | human | 140459 | ASB6 | ankyrin repeat and SOCS box... | NM_177999.3 | 46.7% | 32.3% | 207G>C;401_402ins109;591_592ins563 |
| 4 | mouse | 72323 | Asb6 | ankyrin repeat and SOCS box... | NM_133346.2 | 82.5% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gttcctgcac ggcttccgga ggatcatctt cgagtaccag ccgctggtgg 121 atgcgattct gggctccctg gggatccagg accccgagcg gcaggagtct ctggaccggc 181 ccagttatgt cgccagcgag gagagccgaa tccttgttct cactgagctg ctggagagga 241 aagcccactc tcccttttac caggaaggcg tcagcaacgc cctgctcaag atggctgagc 301 tggggctgac gcgggcggcc gacgttctct tgcggcatgg ggccaatctc aactttgaag 361 acccagtcac ctactacacg gccttgcaca tcgccgtcct gcggaaccag ccggacatgg 421 tggagctgct ggtgcatcac ggggccgacg ttaatcggag ggaccggatc cacgagagta 481 gccccttgga cctggccagc gaggagcctg agcgcctgcc ctgcctgcag cgcctcctgg 541 accttggagc tgatgtcaat gccgctgaca agcatggaaa aactgctctg ctccatgctc 601 tggccagcag cgacggggtg cagatccaca atactgagaa cattcgtctc ttactggaag 661 gaggggcaga cgtcaaggcc accaccaaag atggggacac agtgttcacc tgcatcatct 721 tcctgcttgg tgagaccgtg ggaggggaca aagaggaggc ccagatgatc aaccgcttct 781 gcttccaagt cacacggctg ctgctggcac acggggccga ccccagcgag tgcccagccc 841 acgagtccct cacccacatc tgcctgaaga gctttaaact gcacttccct ctcctgcgct 901 tcctcctgga gtccggagct gcctacaact gctccctgca cggtgcgtcc TGCTGGTCTG 961 GCTTTCACAT CATCTTTGAG AGGCTCTGTT CCCACCCAGG CTGCACGGAA GACGAGAGCC 1021 ATGCGGACCT CCTGCGCAAA GCTGAGACTG TCCTGGATCT CATGGTGACC AACTCTCAGA 1081 AACTCCAGCT GCCCGAAAAC TTCGATATCC ACCCTGTGGG CAGCCTGGCG GAAAAGATCC 1141 AGGCCCTCCA CTTCTCCTTG AGGCAGCTGG AGAGCTATCC CCCGCCCCTC AAGCACCTGT 1201 GCCGTGTGGC CATCCGGCTC TACCTTCAGC CGTGGCCTGT GGATGTGAAG GTCAAAGCCC 1261 TGCCTCTGCC CGACAGGCTG AAGTGGTACC TCCTTAGCGA GCACAGTGGC TCCGTGGAAG 1321 ATGACATCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAGCCA TCTTTGTGTG CCTTTTTTAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt