Construct: ORF TRCN0000478927
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012794.1_s317c1
- Derived from:
- ccsbBroadEn_11772
- DNA Barcode:
- GAGTTTGCTCACGATATCGATTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COG4 (25839)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478927
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25839 | COG4 | component of oligomeric gol... | XM_024450224.1 | 83.7% | 80% | (many diffs) |
| 2 | human | 25839 | COG4 | component of oligomeric gol... | NM_001365426.1 | 82.2% | 82.3% | 1_342del;351G>A;1716G>A |
| 3 | human | 25839 | COG4 | component of oligomeric gol... | NM_015386.3 | 67.4% | 67.5% | 1_768del;777G>A;2142G>A |
| 4 | human | 25839 | COG4 | component of oligomeric gol... | NM_001195139.2 | 65.1% | 65.2% | (many diffs) |
| 5 | human | 25839 | COG4 | component of oligomeric gol... | NR_158212.1 | 54.7% | (many diffs) | |
| 6 | human | 25839 | COG4 | component of oligomeric gol... | XR_933267.1 | 53.5% | (many diffs) | |
| 7 | human | 25839 | COG4 | component of oligomeric gol... | XR_001751889.1 | 53% | (many diffs) | |
| 8 | mouse | 102339 | Cog4 | component of oligomeric gol... | NM_001310607.1 | 67.3% | 71.2% | (many diffs) |
| 9 | mouse | 102339 | Cog4 | component of oligomeric gol... | NM_133973.2 | 61% | 64.5% | (many diffs) |
| 10 | mouse | 102339 | Cog4 | component of oligomeric gol... | XR_387727.3 | 50.9% | (many diffs) | |
| 11 | mouse | 102339 | Cog4 | component of oligomeric gol... | XR_001778388.1 | 35.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1665
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gctagggaca gacatgagtg atcggagagc tgcagtcatc tttgcagata 121 cacttactct tctgtttgaa gggattgccc gcattgtgga gacccaccag ccaatagtgg 181 agacctatta tgggccaggg agactctata ccctgatcaa atatctgcag gtggaatgtg 241 acagacaggt ggagaaggtg gtagacaagt tcatcaagca aagggactac caccagcagt 301 tccggcatgt tcagaacaac ctgatgagaa attctacaac agaaaaaatc gaaccaagag 361 aactggaccc catcctgact gaggtcaccc tgatgaatgc ccgcagtgag ctatacttac 421 gcttcctcaa gaagaggatt agctctgatt ttgaggtggg agactccatg gcctcagagg 481 aagtaaagca agagcaccag aagtgtctgg acaaactcct caataactgc cttttgagct 541 gtaccatgca ggagctaatt ggcttatatg ttaccatgga ggagtacttc atgagggaga 601 ctgtcaataa ggctgtggct ctggacacct atgagaaggg ccagctgaca tccagcatgg 661 tggatgatgt cttctacatt gttaagaagt gcattgggcg ggctctgtcc agctccagca 721 ttgactgtct ctgtgccatg atcaacctcg ccaccacaga gctggagtct gacttcaggg 781 atgttctgtg taataagctg cggatgggct ttcctgccac caccttccag gacatccagc 841 gcggggtgac aagtgccgtg aacatcatgc acagcagcct ccagcaaggc aaatttgaca 901 caaaaggcat cgagagtact gacgaggcga agatgtcctt cctggtgact ctgaacaacg 961 tggaagtctg cagtgaaaac atctccactc tgaagaagac actggagagt gactgcacca 1021 agctcttcag ccagggcatt ggaggggagc aggcccaggc caagtttgac agctgccttt 1081 ctgacttggc cgccgtgtcc aacaaattcc gagacctctt gcaggaaggg ctgacggagc 1141 tcaacagcac agccatcaag ccacaggtgc agccttggat caacagcttt ttctccgtct 1201 cccacaacat cgaggaggaa gaattcaatg actatgaggc caacgaccct tgggtacaac 1261 agttcatcct taacctggag cagcaaatgg cagagttcaa ggccagcctg tccccggtca 1321 tctacgacag cctaaccggc cTCATGACTA GCCTTGTTGC CGTCGAGTTG GAGAAAGTGG 1381 TGCTGAAATC CACCTTTAAC CGGCTGGGTG GTCTGCAGTT TGACAAGGAG CTGAGGTCAC 1441 TCATTGCCTA CCTTACCACG GTGACCACCT GGACCATCCG AGACAAGTTT GCCCGGCTCT 1501 CCCAGATGGC CACCATCCTC AATCTGGAGC GGGTGACCGA GATCCTCGAT TACTGGGGAC 1561 CCAATTCCGG CCCATTGACG TGGCGCCTCA CCCCTGCTGA AGTGCGCCAG GTGCTGGCCC 1621 TGCGGATAGA CTTCCGCAGT GAAGATATCA AGAGGCTGCG CCTGTACCCA ACTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA GAGTTTGCTC ACGATATCGA TTACACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt