Transcript: Mouse NM_133973.2

Mus musculus component of oligomeric golgi complex 4 (Cog4), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Cog4 (102339)
Length:
2754
CDS:
28..2385

Additional Resources:

NCBI RefSeq record:
NM_133973.2
NBCI Gene record:
Cog4 (102339)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133973.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000125508 CGACTCTTTACCCTGATCAAA pLKO.1 919 CDS 100% 5.625 7.875 N Cog4 n/a
2 TRCN0000125506 CGGGTGACTGAGATACTAGAT pLKO.1 2248 CDS 100% 4.950 6.930 N Cog4 n/a
3 TRCN0000349157 CGGGTGACTGAGATACTAGAT pLKO_005 2248 CDS 100% 4.950 6.930 N Cog4 n/a
4 TRCN0000125505 GCGCGAAGTGAACTGTACTTA pLKO.1 1117 CDS 100% 5.625 4.500 N Cog4 n/a
5 TRCN0000316190 GCGCGAAGTGAACTGTACTTA pLKO_005 1117 CDS 100% 5.625 4.500 N Cog4 n/a
6 TRCN0000125507 CCTGAGCAAGTTCTCGGAATA pLKO.1 720 CDS 100% 10.800 7.560 N Cog4 n/a
7 TRCN0000316117 CCTGAGCAAGTTCTCGGAATA pLKO_005 720 CDS 100% 10.800 7.560 N Cog4 n/a
8 TRCN0000146584 CCATTGAAAGTAAGATGGTCA pLKO.1 233 CDS 100% 2.640 1.848 N COG4 n/a
9 TRCN0000125504 GCCAGACTTCAGGACCTGTTT pLKO.1 2400 3UTR 100% 4.950 2.970 N Cog4 n/a
10 TRCN0000349158 GCCAGACTTCAGGACCTGTTT pLKO_005 2400 3UTR 100% 4.950 2.970 N Cog4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133973.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11772 pDONR223 100% 61% 64.5% None (many diffs) n/a
2 ccsbBroad304_11772 pLX_304 0% 61% 64.5% V5 (many diffs) n/a
3 TRCN0000478927 GAGTTTGCTCACGATATCGATTAC pLX_317 20.6% 61% 64.5% V5 (many diffs) n/a
4 ccsbBroadEn_11773 pDONR223 100% 61% 64.4% None (many diffs) n/a
5 ccsbBroad304_11773 pLX_304 0% 61% 64.4% V5 (many diffs) n/a
6 TRCN0000473203 GGCAGCCGTAAACCACCACTTTGC pLX_317 19.5% 61% 64.4% V5 (many diffs) n/a
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