Construct: ORF TRCN0000478929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009692.2_s317c1
- Derived from:
- ccsbBroadEn_02114
- DNA Barcode:
- GGTGAATCGTCTTCGATGACGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DLGAP2 (9228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9228 | DLGAP2 | DLG associated protein 2 | NM_001346810.2 | 92.4% | 92.4% | 1_240del |
| 2 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | XM_011242055.2 | 82.3% | 88.8% | (many diffs) |
| 3 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | XM_006508782.3 | 77% | 83.1% | (many diffs) |
| 4 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | XM_006508781.3 | 76.4% | 82.5% | (many diffs) |
| 5 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | XM_017312785.1 | 76.4% | 82.5% | (many diffs) |
| 6 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | NM_172910.3 | 76.1% | 82.2% | (many diffs) |
| 7 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | NM_001145965.1 | 76.1% | 82.1% | (many diffs) |
| 8 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | XM_017312786.1 | 74.9% | 81% | (many diffs) |
| 9 | mouse | 244310 | Dlgap2 | discs, large (Drosophila) h... | XM_006508783.3 | 74.9% | 80.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2994
- ORF length:
- 2925
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagggcctt tccggaagtc ggacccagcc gccgctgtgt tccgggcaca 121 cgtgtggtct ggcgcccccg gaggactgcg agcacctgca ccacgggccc gacgcgcggc 181 cgccctacct gctgagcccc gccgacagct gccccggggg gcgccaccgc tgctcgccgc 241 gcagctcggt gcactcggag tgcgtgatga tgccggtggt gctgggcgac cacgtgtcca 301 gcagcacctt cccgcggatg cactacagct cgcactacga cacgcgcgac gactgcgctg 361 tggcccacgc gggcgccaag atcaaccgca tcccggccaa cctgctggac cagttcgaga 421 agcagctgcc gctgcaccgg gacggcttcc acacgctgca gtaccagagg acgtccgcgg 481 ccgccgagca gcgcagcgag agccccgggc ggatccgcca cctggtacac tccgtgcaga 541 agctcttcac caagtcgcac tcgctggagg gctcctccaa aagcaacgcc aacggcacca 601 aggcggacgg ccgggcggac gaccaccacc acgcccacca cgccaagcac agcaagagga 661 gcaagagcaa ggagcgcaag ccggagggca agccccggcc cggcatgagc agctggtgga 721 gctcggacga caacctggac agcgacagca cctatcggac gcccagcgtg ctcaaccggc 781 accacctggg ccccgtggcc cactgctacc ccgacgcgct gcagagcccc ttcggggacc 841 tgtccctcaa gacctccaag agcaacaacg acgtcaagtg ctcggcctgt gaggggttgg 901 cgctgacgcc cgacgccaag tacctgaagc gcagctcctg gtctacgctg acggtcagcc 961 aggccaagga ggcctaccgc aagagctcgc tgaacctgga caagccgctg ctgcaccagg 1021 acgccaagcc cgccctgagg ccgtgccact acctccaggt acctcaggat gagtggggag 1081 ggtaccccac cggtggcaaa gatgaggaga ttccctgcag gagaatgaga agtgggagtt 1141 acattaaagc catgggggac gaggagagcg gagagtcaga ctccagcccc aagacatcac 1201 caaagtcggc aatcctacca gagccgctgc tgaagtccat cggacagaga ccgcttggag 1261 agcaccagac ccagacctac ctgcaagctg caagcgatgt gcctgtggga cacagcctgg 1321 accccgctgc gaactacaac tccccgaaat tccgctcccg gaaccagagc tacatgaggg 1381 ccgtcagcac cctgagccag gccagctgcg tgagccaggt gagcgaggcg gagatcaatg 1441 ggcaattcga gtccgtgtgc gagtccgtct tcagtgaagt tgaatctcag gccatggatg 1501 ccctcgacct cccgggatgt ttccgaacaa ggagtcacag ctaccttcga gccattcaag 1561 ccggctactc ccaagatgac gaatgtattc ccatgatgac accctctgac atcacctcca 1621 ccatcaggtc aacagcagct gtctcatata caaattacaa gaaaacgccc ccaccggtgc 1681 cccctcggac cacctccaag cctctgatct cggtgacggc gcagagcagc accgaatcca 1741 cccaggacgc ctaccaggac agccgcgcac agaggatgtc cccgtggccc caggacagcc 1801 gcggcctcta caactccacg gacagcctgg acagcaacaa ggccatgaac ctcgcgctgg 1861 aaacggccgc tgcccagcgc cacctgccag agagccagag cagctctgtg cggaccagcg 1921 acaaggccat cctggtgtcc aaggcggagg agctcctcaa gagccgctgc tcctccatcg 1981 ggattcagga ttctgaattc ccagagcatc agccataccc aaggtcagat gtggaaacgg 2041 ccacagattc tgacacggag agccgcggtc tgcgggaata ccactctgtc ggggtgcaag 2101 tggaagatga gaagcgacac ggacgtttta aacgttctaa cagcgtcacg gccgccgtcc 2161 aagctgacct ggagctggag gggttcccag gccacatcac cacggaggac aaaggccttc 2221 agttcggctc atccttccag cggcactccg agcccagcac ccccacccag tacagcgcgg 2281 tgagaactgt acggacccag gggctcttca gctatagaga agactatcgg acccaagtgg 2341 acacctccac cctgccccct ccagacccct ggctggagcc cgccatcgac acggtagaga 2401 ctgggaggat gtctccgtgc cgcagggatg gctcgtggtt tttgaagctg ctgcacgcag 2461 agacaaagag gatggaaggc tggtgcaaag agatggagag agaggcggag gagaacgacc 2521 tctcggagga aattctcggt aaaatcagga gtgctgttgg gagtgcccag cttctcatgt 2581 cccagaaatt ccagcagttt tattggcttt gccaacagaa tatggacccc agcgccatgc 2641 cgaggccgac gtcgcaggac ctggccggct actgggacat gctgcagctc tccattgagg 2701 acgtcagcat gaagttcGAC GAGCTGCAGC GGCTGCGGCT CAACGACTGG AAGATGATGG 2761 AGTCCCCGGA AAGAAAGGAA GAAAGAAAGG TCCCGCCTCC AATACCAAAG AAGCCTCCCA 2821 AGGGGAAGTT TCCCATCACA AGAGAAAAAT CCCTGGACCT GCCCGACAGA CAACGCCAGG 2881 AAGCCCGGAG GCGCCTCATG GCCGCCAAGC GAGCGGCGTC CTTCCGGCAG AATTCCGCCT 2941 CCGAGCGCGC GGACAGCATC GAGATCTACA TCCCCGAGGC CCAGACCCGG CTCTTGCCAA 3001 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3061 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3121 TATCTTGTGG AAAGGACGAG GTGAATCGTC TTCGATGACG GTCACGCGTT AAGTCgacaa 3181 tcaacctctg gattacaaaa tttgtgaaag att