Construct: ORF TRCN0000478931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003863.2_s317c1
- Derived from:
- ccsbBroadEn_12376
- DNA Barcode:
- GCTCAAAGTGGATCAGTACATAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSHZ3 (57616)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478931
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57616 | TSHZ3 | teashirt zinc finger homeob... | NM_020856.4 | 83% | 83% | 1_549del |
| 2 | mouse | 243931 | Tshz3 | teashirt zinc finger family... | XM_006539965.2 | 76.7% | 83.2% | (many diffs) |
| 3 | mouse | 243931 | Tshz3 | teashirt zinc finger family... | XM_006539966.3 | 76.7% | 83.2% | (many diffs) |
| 4 | mouse | 243931 | Tshz3 | teashirt zinc finger family... | XM_017322238.1 | 76.7% | 83.2% | (many diffs) |
| 5 | mouse | 243931 | Tshz3 | teashirt zinc finger family... | NM_172298.2 | 73.5% | 79.7% | (many diffs) |
| 6 | mouse | 243931 | Tshz3 | teashirt zinc finger family... | XM_006539963.1 | 72.8% | 78.9% | (many diffs) |
| 7 | mouse | 243931 | Tshz3 | teashirt zinc finger family... | XM_006539964.3 | 72.8% | 78.9% | (many diffs) |
| 8 | mouse | 243931 | Tshz3 | teashirt zinc finger family... | XM_006539961.3 | 70.6% | 76.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2763
- ORF length:
- 2694
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctcccggag cccagcctct tcagcaccgt gcagctgtac cggcagagca 121 gcaagctcta tggctccatc ttcacggggg ccagcaagtt ccgctgtaag gactgcagcg 181 ctgcctacga caccctggtg gagttgacgg tgcacatgaa cgagacgggg cattaccgcg 241 acgacaacca tgagaccgat aacaacaacc ccaagcgctg gtccaagcct cgcaaacgct 301 ccttgctgga aatggaaggg aaggaagacg cccagaaggt gctgaagtgc atgtactgtg 361 gccactcctt tgagtccctg caggatttga gtgtccatat gatcaaaaca aaacactacc 421 aaaaagtgcc tctgaaggaa cccgtcactc ctgtcgccgc caaaatcatc cctgccactc 481 ggaagaaagc ttccctggag ctggagctcc ccagctcccc agattccaca ggtggaaccc 541 ccaaagccac catctcagac accaacgatg cacttcagaa gaactccaac ccttacatca 601 cgccaaataa tcggtacggc caccagaatg gggccagcta tgcatggcac tttgaggccc 661 ggaagtcgca gatcctgaag tgcatggagt gtgggagctc gcatgacacc ctgcaggagc 721 tcactgccca catgatggtc actggccact tcatcaaggt caccaactct gctatgaaaa 781 aggggaagcc cattgtggag acgcctgtca cacctaccat cacaaccctg ctggatgaga 841 aggtccagtc cgtgcccctg gcagccacca ccttcacgtc cccctccaat acacctgcca 901 gcatctcccc aaaactgaat gtggaggtca agaaggaagt cgacaaggag aaagcggtca 961 ctgacgagaa acctaagcaa aaagacaagc ctggcgaaga agaggagaag tgtgacatct 1021 cttccaaata ccattacttg actgaaaatg acttagaaga gagtcccaag ggggggcttg 1081 atatcctcaa atccttggaa aacacagtga catccgcaat caacaaggcc cagaacggca 1141 ctcctagctg ggggggctat cccagcatcc atgccgccta ccaacttccc aacatgatga 1201 agttgtccct gggctcgtcg gggaagagca cgcccctgaa acccatgttt ggcaacagtg 1261 agattgtctc cccgacgaaa aaccagaccc tggtctctcc acccagcagc cagacgtccc 1321 ccatgcccaa gacaaacttt catgccatgg aggagctggt gaaaaaggtc actgagaaag 1381 ttgccaaagt ggaggagaag atgaaggagc cggatgggaa gctttccccg cccaagcggg 1441 ccactccctc cccatgtagc agcgaagtcg gggaacccat caagatggag gcatccagcg 1501 atgggggctt ccgcagccag gagaacagcc ccagcccccc gcgggatggg tgcaaggatg 1561 ggagccccct cgctgagccg gtggagaatg gcaaggagct ggtgaagccc ctagccagca 1621 gtttgagtgg cagcacggcc atcatcaccg accacccgcc tgaacagcct tttgttaacc 1681 ctttgagcgc cctgcagtca gtcatgaaca ttcacctggg caaggccgcc aagccctccc 1741 tgcctgccct ggaccccatg agcatgcttt tcaagatgag caacagcctg gcggagaagg 1801 ctgctgtggc caccccgccg cccctgcagt ccaagaaggc agaccacctc gaccgctatt 1861 tctaccacgt caacaacgac cagcccatag acttgacaaa agggaagagt gacaaaggct 1921 gctccttggg ttcagtgctt ctgtcaccca cgtccacagc cccggcaacc tcctcatcca 1981 cggtgacaac ggcaaagaca tctgccgtcg tatcattcat gtcaaactcg ccgctacgcg 2041 agaatgcctt gtcagatata tccgatatgc tgaagaactt gacagagagc cacacgtcaa 2101 aatcctccac tccttccagc atctccgaga agtctgacat tgacggggcc actctggagg 2161 aggctgagga gtcgacgccc gcccagaaga ggaagggccg ccagtcaaac tggaaccccc 2221 agcacctcct gatcctccag gcccagtttg ccgccagcct ccggcagacc tcagaaggga 2281 agtacatcat gtcagacctg agcccccagg agcggatgca tatctccagg ttcaccgggc 2341 tgtccatgac caccatcagc cactGGCTGG CCAACGTGAA ATACCAGCTT CGAAGGACAG 2401 GTGGAACAAA GTTCCTCAAA AACTTGGACA CTGGCCACCC CGTCTTCTTT TGTAACGATT 2461 GTGCGTCCCA AATCAGGACT CCTTCCACGT ACATCAGTCA CCTAGAGTCA CACTTAGGCT 2521 TCCGGCTACG GGACTTATCC AAACTGTCCA CCGAACAGAT TAACAGTCAG ATAGCACAAA 2581 CCAAGTCACC GTCAGAAAAA ATGGTGACGT CCTCCCCCGA GGAAGACCTG GGGACTTCCT 2641 ATCAGTGCAA ACTTTGCAAT CGGACCTTTG CCAGCAAGCA CGCTGTTAAA CTTCACCTTA 2701 GCAAAACACA CGGGAAATCT CCGGAAGACC ACCTTCTGTA TGTCTCTGAG TTAGAGAAGC 2761 AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2821 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2881 GGCTTTATAT ATCTTGTGGA AAGGACGAGC TCAAAGTGGA TCAGTACATA AAACGCGTTA 2941 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt