Construct: ORF TRCN0000478943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009765.1_s317c1
- Derived from:
- ccsbBroadEn_01697
- DNA Barcode:
- CTATCCAACCGCCATGACGAGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPD52 (7163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478943
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7163 | TPD52 | tumor protein D52 | NM_005079.4 | 100% | 100% | |
| 2 | human | 7163 | TPD52 | tumor protein D52 | NM_001025253.3 | 88.8% | 88.8% | 387_455del |
| 3 | human | 7163 | TPD52 | tumor protein D52 | NM_001025252.3 | 81.3% | 79.4% | (many diffs) |
| 4 | human | 7163 | TPD52 | tumor protein D52 | NM_001287144.2 | 78.8% | 78.8% | 435_436ins117 |
| 5 | human | 7163 | TPD52 | tumor protein D52 | NM_001287143.2 | 78.1% | 76.3% | (many diffs) |
| 6 | human | 7163 | TPD52 | tumor protein D52 | NM_001287142.1 | 76.5% | 74.3% | (many diffs) |
| 7 | human | 7163 | TPD52 | tumor protein D52 | NM_001287140.2 | 73.7% | 72% | (many diffs) |
| 8 | human | 7163 | TPD52 | tumor protein D52 | NR_105035.2 | 13.4% | 1_121del;141_210del;744_4117del | |
| 9 | human | 7163 | TPD52 | tumor protein D52 | NR_105036.2 | 13.3% | (many diffs) | |
| 10 | human | 7163 | TPD52 | tumor protein D52 | NR_105037.2 | 13.1% | (many diffs) | |
| 11 | human | 7163 | TPD52 | tumor protein D52 | NR_105034.2 | 12.3% | (many diffs) | |
| 12 | human | 7163 | TPD52 | tumor protein D52 | NR_105033.2 | 12% | (many diffs) | |
| 13 | mouse | 21985 | Tpd52 | tumor protein D52 | NM_009412.2 | 83% | 85.9% | (many diffs) |
| 14 | mouse | 21985 | Tpd52 | tumor protein D52 | NM_001025264.1 | 79.2% | 81.9% | (many diffs) |
| 15 | mouse | 21985 | Tpd52 | tumor protein D52 | XM_006530070.3 | 73.9% | 76.4% | (many diffs) |
| 16 | mouse | 21985 | Tpd52 | tumor protein D52 | XM_006530071.3 | 64.6% | 68.2% | (many diffs) |
| 17 | mouse | 21985 | Tpd52 | tumor protein D52 | XM_006530066.3 | 62.7% | 64.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccgcggcgag caaggtctgc tgagaacaga cccagtccct gaggaaggag 121 aagatgttgc tgccacgatc agtgccacag agaccctctc ggaagaggag caggaagagc 181 taagaagaga acttgcaaag gtagaagaag aaatccagac tctgtctcaa gtgttagcag 241 caaaagagaa gcatctagca gagatcaagc ggaaacttGG AATCAATTCT CTACAGGAAC 301 TAAAACAGAA CATTGCCAAA GGGTGGCAAG ACGTGACAGC AACATCTGCT TACAAGAAGA 361 CATCTGAAAC CTTATCCCAG GCTGGACAGA AGGCCTCAGC TGCTTTTTCG TCTGTTGGCT 421 CAGTCATCAC CAAAAAGCTG GAAGATGTAA AAAACTCCCC AACTTTTAAA TCATTTGAAG 481 AAAAGGTCGA AAACTTAAAG TCTAAAGTAG GGGGAACCAA GCCTGCTGGT GGTGATTTTG 541 GAGAAGTCTT GAATTCGGCT GCAAATGCTA GTGCCACCAC CACGGAGCCT CTTCCAGAAA 601 AGACACAGGA GAGCCTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTATCCA ACCGCCATGA 781 CGAGAGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt