Construct: ORF TRCN0000478946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006761.2_s317c1
- Derived from:
- ccsbBroadEn_06515
- DNA Barcode:
- TTCCCGTGGAGGCCCGTGGGGCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STMN1 (3925)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3925 | STMN1 | stathmin 1 | NM_005563.4 | 99.7% | 99.3% | 133C>T |
| 2 | human | 3925 | STMN1 | stathmin 1 | NM_203399.1 | 99.7% | 99.3% | 133C>T |
| 3 | human | 3925 | STMN1 | stathmin 1 | NM_203401.1 | 99.7% | 99.3% | 133C>T |
| 4 | human | 3925 | STMN1 | stathmin 1 | NM_001145454.3 | 78.8% | 68.9% | (many diffs) |
| 5 | mouse | 16765 | Stmn1 | stathmin 1 | NM_019641.4 | 88.8% | 97.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcttctgat atccaggtga aagaactgga gaagcgtgcc tcaggccagg 121 cttttgagct gattctcagc cctcggtcaa aagaatctgt tccagaattc cccctttccc 181 ctccaaagaa gaaggatttT TCCCTGGAGG AAATTCAGAA GAAATTAGAA GCTGCAGAAG 241 AAAGACGCAA GTCCCATGAA GCTGAGGTCT TGAAGCAGCT GGCTGAGAAA CGAGAGCACG 301 AGAAAGAAGT GCTTCAGAAG GCAATAGAAG AGAACAACAA CTTCAGTAAA ATGGCAGAAG 361 AGAAACTGAC CCACAAAATG GAAGCTAATA AAGAGAACCG AGAGGCACAA ATGGCTGCCA 421 AACTGGAACG TTTGCGAGAG AAGGATAAGC ACATTGAAGA AGTGCGGAAG AACAAAGAAT 481 CCAAAGACCC TGCTGACGAG ACTGAAGCTG ACTACCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 661 CCCGTGGAGG CCCGTGGGGC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt