Construct: ORF TRCN0000478967
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004780.1_s317c1
- Derived from:
- ccsbBroadEn_03875
- DNA Barcode:
- TGGGAACGGACATTTAACCCGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHA1 (59338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478967
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | NM_001001974.3 | 100% | 100% | |
| 2 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | NM_021622.4 | 100% | 100% | |
| 3 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_005270016.2 | 100% | 100% | |
| 4 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016480.1 | 100% | 100% | |
| 5 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016481.1 | 100% | 100% | |
| 6 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016482.1 | 100% | 100% | |
| 7 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448111.1 | 100% | 100% | |
| 8 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448112.1 | 100% | 100% | |
| 9 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448113.1 | 100% | 100% | |
| 10 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448114.1 | 100% | 100% | |
| 11 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | NM_001330178.2 | 88.4% | 72.3% | 901_941del;1149_1150ins104 |
| 12 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016486.1 | 88.4% | 72.3% | 901_941del;1149_1150ins104 |
| 13 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016487.1 | 88.4% | 72.3% | 901_941del;1149_1150ins104 |
| 14 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016488.1 | 88.4% | 72.3% | 901_941del;1149_1150ins104 |
| 15 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448115.1 | 88.4% | 72.3% | 901_941del;1149_1150ins104 |
| 16 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448116.1 | 88.4% | 72.3% | 901_941del;1149_1150ins104 |
| 17 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448117.1 | 88.4% | 72.3% | 901_941del;1149_1150ins104 |
| 18 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_005270019.2 | 86.3% | 76.2% | 901_971del;1179_1180ins104 |
| 19 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016483.1 | 86.3% | 76.2% | 901_971del;1179_1180ins104 |
| 20 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016484.1 | 86.3% | 76.2% | 901_971del;1179_1180ins104 |
| 21 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016485.1 | 86.3% | 76.2% | 901_971del;1179_1180ins104 |
| 22 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016479.2 | 84.3% | 75% | 901_1006del;1218_1219ins100 |
| 23 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_011540017.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 24 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_011540018.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 25 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_011540019.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 26 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_011540021.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 27 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_011540022.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 28 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_011540024.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 29 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016478.2 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 30 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448107.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 31 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448108.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 32 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448109.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 33 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_024448110.1 | 82.4% | 74.6% | 901_1036del;1248_1249ins100 |
| 34 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016489.2 | 81.2% | 73.3% | (many diffs) |
| 35 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | NM_001195608.1 | 79.8% | 74.5% | (many diffs) |
| 36 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_005270021.5 | 79.8% | 74.5% | (many diffs) |
| 37 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016490.1 | 79.8% | 74.5% | (many diffs) |
| 38 | human | 59338 | PLEKHA1 | pleckstrin homology domain ... | XM_017016491.1 | 79.8% | 74.5% | (many diffs) |
| 39 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507145.1 | 88.3% | 92% | (many diffs) |
| 40 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | NM_001346515.1 | 78.9% | 71.1% | (many diffs) |
| 41 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507149.3 | 78.9% | 71.1% | (many diffs) |
| 42 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | NM_133942.2 | 77.5% | 80.1% | (many diffs) |
| 43 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507146.1 | 77% | 73.3% | (many diffs) |
| 44 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507147.1 | 77% | 69.5% | (many diffs) |
| 45 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507140.1 | 75.3% | 72.7% | (many diffs) |
| 46 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507141.3 | 75.3% | 72.7% | (many diffs) |
| 47 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507142.3 | 75.3% | 72.7% | (many diffs) |
| 48 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507143.3 | 75.3% | 72.7% | (many diffs) |
| 49 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_017321904.1 | 75.3% | 72.7% | (many diffs) |
| 50 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507152.1 | 74.6% | 72.7% | (many diffs) |
| 51 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507153.2 | 73% | 72.6% | (many diffs) |
| 52 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507150.1 | 65.3% | 61.6% | (many diffs) |
| 53 | mouse | 101476 | Plekha1 | pleckstrin homology domain ... | XM_006507151.3 | 65.3% | 61.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ttatgtggat cgtcagaatc gcatttgtgg ttttctagac attgaagaaa 121 atgaaaacag tgggaaattt cttcgaaggt acttcatact ggataccaga gaagatagtt 181 tcgtgtggta catggataat ccacagaacc taccttctgg atcatcacgt gttggagcca 241 ttaagcttac ctacatttca aaggttagcg atgctactaa gctaaggcca aaggcggagt 301 tctgttttgt tatgaatgca ggaatgagga agtacttcct acaagccaat gatcagcagg 361 acctagtgga atgggtaaat gtgttaaaca aagctataaa aattacagta ccaaagcagt 421 cagactcaca gcctaattct gataacctaa gtcgccatgg tgaatgtggg aaaaagcaag 481 tgtcttacag aactgatatt gttggtggcg tacccatcat tactcccact cagaaagaag 541 aagtaaatga atgtggtgaa agtattgaca gaaataatct gaaacggtca caaagccatc 601 ttccttactt tactcctaaa ccacctcaag atagtgcggt tatcaaagct ggatattgtg 661 taaaacaagg agcagtgatg aaaaactgga agagaagata ttttcaattg gatgaaaaca 721 caataggcta cttcaaatct gaactggaaa aggaacctct tcgtgtaata ccacttaaag 781 aggttcataa agtccaggaa tgtaagcaaa gcgacataat gatgagggac aaccTCTTTG 841 AAATTGTAAC AACGTCTCGA ACTTTCTATG TGCAGGCTGA TAGCCCTGAA GAGATGCACA 901 GTTGGATTAA AGCAGTCTCT GGCGCCATTG TAGCACAGCG GGGTCCCGGC AGATCTGCGT 961 CTTCTGAGCA TCCCCCCGGT CCTTCAGAAT CCAAACACGC TTTCCGTCCT ACCAACGCAG 1021 CCACCGCCAC CTCACATTCC ACAGCCTCTC GCAGCAACTC TTTGGTCTCA ACCTTTACCA 1081 TGGAGAAGCG AGGATTTTAC GAGTCTCTTG CCAAGGTCAA GCCAGGGAAC TTCAAGGTCC 1141 AGACTGTCTC TCCAAGAGAA CCAGCTTCCA AAGTGACTGA ACAAGCTCTG TTAAGACCTC 1201 AAAGTAAAAA TGGCCCTCAG GAAAAAGATT GTGACCTAGT AGACTTGGAC GATGCGAGCC 1261 TTCCGGTCAG TGACGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGGAAC GGACATTTAA 1441 CCCGGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt