Transcript: Human XM_011540017.1

PREDICTED: Homo sapiens pleckstrin homology domain containing A1 (PLEKHA1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PLEKHA1 (59338)
Length:
4210
CDS:
447..1697

Additional Resources:

NCBI RefSeq record:
XM_011540017.1
NBCI Gene record:
PLEKHA1 (59338)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011540017.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000155104 GACCTAGTGGAATGGGTAAAT pLKO.1 741 CDS 100% 13.200 18.480 N PLEKHA1 n/a
2 TRCN0000343758 GACCTAGTGGAATGGGTAAAT pLKO_005 741 CDS 100% 13.200 18.480 N PLEKHA1 n/a
3 TRCN0000156119 CAAAGGTTAGCGATGCTACTA pLKO.1 640 CDS 100% 4.950 6.930 N PLEKHA1 n/a
4 TRCN0000157076 GACCTAGTAGACTTGGACGAT pLKO.1 1750 3UTR 100% 2.640 3.696 N PLEKHA1 n/a
5 TRCN0000352964 GACCTAGTAGACTTGGACGAT pLKO_005 1750 3UTR 100% 2.640 3.696 N PLEKHA1 n/a
6 TRCN0000158406 CCAGCTTCCAAAGTGACTGAA pLKO.1 1678 CDS 100% 4.950 3.465 N PLEKHA1 n/a
7 TRCN0000343822 CCAGCTTCCAAAGTGACTGAA pLKO_005 1678 CDS 100% 4.950 3.465 N PLEKHA1 n/a
8 TRCN0000154730 GAATGCAGGAATGAGGAAGTA pLKO.1 695 CDS 100% 4.950 3.465 N PLEKHA1 n/a
9 TRCN0000155127 CAAGCTCTGTTAAGACCTCAA pLKO.1 1699 3UTR 100% 4.050 2.835 N PLEKHA1 n/a
10 TRCN0000154842 GCACAGTTGGATTAAAGCAGT pLKO.1 1277 CDS 100% 2.640 1.848 N PLEKHA1 n/a
11 TRCN0000343759 GCACAGTTGGATTAAAGCAGT pLKO_005 1277 CDS 100% 2.640 1.848 N PLEKHA1 n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3236 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3236 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011540017.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03875 pDONR223 100% 82.4% 74.6% None 901_1036del;1248_1249ins100 n/a
2 ccsbBroad304_03875 pLX_304 0% 82.4% 74.6% V5 901_1036del;1248_1249ins100 n/a
3 TRCN0000478967 TGGGAACGGACATTTAACCCGGCC pLX_317 36.8% 82.4% 74.6% V5 901_1036del;1248_1249ins100 n/a
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