Construct: ORF TRCN0000478988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006433.2_s317c1
- Derived from:
- ccsbBroadEn_01667
- DNA Barcode:
- TCCCTAATTGAACATCCTTTGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGIF1 (7050)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_001278684.2 | 100% | 100% | |
| 2 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_003244.4 | 100% | 100% | |
| 3 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_173208.3 | 100% | 100% | |
| 4 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | XM_017025958.1 | 100% | 100% | |
| 5 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_001278682.2 | 98% | 96.7% | (many diffs) |
| 6 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_173207.4 | 94.3% | 93.3% | (many diffs) |
| 7 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_001278686.2 | 92.6% | 92.6% | 0_1ins60 |
| 8 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_001374396.1 | 92.6% | 92.6% | 0_1ins60 |
| 9 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_001374397.1 | 92.6% | 92.6% | 0_1ins60 |
| 10 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_170695.5 | 92.6% | 92.6% | 0_1ins60 |
| 11 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_173209.3 | 92.6% | 92.6% | 0_1ins60 |
| 12 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_173210.4 | 92.6% | 92.6% | 0_1ins60 |
| 13 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_173211.2 | 92.6% | 92.6% | 0_1ins60 |
| 14 | human | 7050 | TGIF1 | TGFB induced factor homeobox 1 | NM_174886.3 | 92.6% | 92.6% | 0_1ins60 |
| 15 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | NM_009372.3 | 87.6% | 89.7% | (many diffs) |
| 16 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | XM_006524031.3 | 86.8% | 88.9% | (many diffs) |
| 17 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | XM_011246363.2 | 85.3% | 87.6% | (many diffs) |
| 18 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | NM_001164074.1 | 81% | 83.8% | (many diffs) |
| 19 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | NM_001164076.1 | 81% | 83.8% | (many diffs) |
| 20 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | NM_001164077.1 | 81% | 83.8% | (many diffs) |
| 21 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | XM_006524032.2 | 81% | 83.8% | (many diffs) |
| 22 | mouse | 21815 | Tgif1 | TGFB-induced factor homeobox 1 | NM_001164075.1 | 77.3% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 882
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aggcaagaaa ggtattgttg cagcatctgg cagtgagact gaggatgagg 121 acagcatgga cattcccttg gacctttctt catccgctgg ctcaggcaag agaaggagaa 181 ggggcaacct acccaaggag tctgtgcaga ttcttcggga ttggctgtat gagcaccgtt 241 acaatgccta tccttcagag caagaaaaag cgttgctgtc ccagcaaaca cacctgtcta 301 cgctacaggt ctgtaactgg ttcatcaacg cccgccgcag gctcctccct gacatgctga 361 gaaaggatgg caaagatcca aatcagttca caatttcccg ccgtggggcc aagatttcTG 421 AAACGAGCTC TGTGGAGTCC GTGATGGGCA TCAAAAACTT CATGCCAGCT CTAGAGGAGA 481 CCCCATTTCA TTCCTGTACA GCTGGGCCAA ACCCAACCCT AGGGAGGCCA CTGTCTCCTA 541 AGCCGTCATC CCCGGGATCA GTTTTGGCTC GTCCATCAGT GATCTGCCAT ACCACTGTGA 601 CTGCATTGAA AGATGTCCCT TTCTCTCTCT GCCAGTCGGT CGGTGTGGGA CAAAACACAG 661 ATATACAGCA GATAGCGGCC AAAAACTTCA CAGACACCTC TCTCATGTAC CCAGAGGACA 721 CTTGTAAATC TGGACCAAGT ACGAATACAC AGAGTGGTCT TTTCAACACT CCTCCCCCTA 781 CTCCACCGGA CCTCAACCAG GACTTCAGTG GATTTCAGCT TCTAGTGGAT GTTGCACTCA 841 AACGGGCTGC AGAGATGGAG CTTCAGGCAA AACTTACAGC TAACCCAACT TTCTTGTACA 901 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 961 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1021 AGGACGATCC CTAATTGAAC ATCCTTTGCG TACGCGTTAA GTCgacaatc aacctctgga 1081 ttacaaaatt tgtgaaagat t