Construct: ORF TRCN0000478996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012854.1_s317c1
- Derived from:
- ccsbBroadEn_12649
- DNA Barcode:
- CCCAATGCCATACTCTGATCGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERMP1 (79956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | XM_017015139.2 | 37.6% | 37.6% | 1_1575del |
| 2 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | XM_005251587.2 | 36.9% | 35% | (many diffs) |
| 3 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | NM_024896.3 | 35% | 35% | 1_1761del |
| 4 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | XR_428431.3 | 27.3% | 1_1804del;2756_3478del | |
| 5 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | XR_929337.2 | 26.9% | 1_1804del;2756_3525del | |
| 6 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | XR_929338.2 | 19% | 1_1804del;2430_2478del;2805_4999del | |
| 7 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | XR_929340.2 | 17.6% | (many diffs) | |
| 8 | human | 79956 | ERMP1 | endoplasmic reticulum metal... | XR_001746382.2 | 17.4% | (many diffs) | |
| 9 | mouse | 226090 | Ermp1 | endoplasmic reticulum metal... | NM_001081213.1 | 30% | 31.1% | (many diffs) |
| 10 | mouse | 226090 | Ermp1 | endoplasmic reticulum metal... | XM_006526990.3 | 29.9% | 31.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1017
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tattccttat ctttatgcat tgtacctcat ctgggcagta tttgagatgt 121 ttacccctat cctcgggaga agtggttctg aaatcccacc tgatgttgtg ctggcatcca 181 ttttggctgg ctgtacaatg attctctcgt cctattttat taacttcatc taccttgcca 241 agagcacaaa aaaaaccatg ctaactttaa ctttggtatg tgcaattaca ttcctccttg 301 tttgcagtgg aacatttttt ccatatagct ccaatcctgc taatccgaag ccaaagagag 361 tgtttcttca gcatatgact agaacattcc atgacttgga aggaaatgca gttaaacggg 421 actctggaat atggatcaat gggtttgatt atactggaat ttctcacata acccctcaca 481 ttcctgagat caatgatagt atccgagctc actgtgagga gaatgcacct ctttgtggtt 541 ttccttggta tcttccagtg cactttctga tcaggaaaaa ctggtatctt cctgccccag 601 aagtttctcc aagaaatcct ccTCATTTCC GACTCATATC CAAAGAACAG ACACCTTGGG 661 ATTCTATAAA ATTGACTTTT GAAGCAACAG GACCAAGCCA TATGTCCTTC TATGTTCGAG 721 CCCACAAAGG GTCAACACTT TCTCAGTGGT CTCTTGGCAA TGGCACCCCA GTCACAAGTA 781 AAGGAGGAGA CTACTTTGTC TTTTACTCCC ATGGACTCCA GGCCTCTGCA TGGCAGTTCT 841 GGATAGAAGT GCAGGTTTCA GAAGAACATC CTGAAGGAAT GGTCACCGTG GCCATTGCTG 901 CCCACTATCT GTCTGGGGAA GACAAGAGAT CCCCTCAACT GGATGCTCTG AAGGAAAAGT 961 TCCCAGATTG GACATTTCCC TCTGCCTGGG TGTGCACCTA CGATCTCTTT GTATTTTACC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GACCCAATGC CATACTCTGA TCGGCCACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt