Construct: ORF TRCN0000479009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011131.2_s317c1
- Derived from:
- ccsbBroadEn_00705
- DNA Barcode:
- AGTCAGTTCTCCTAAGGACCGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2A1 (2957)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2957 | GTF2A1 | general transcription facto... | NM_015859.4 | 100% | 100% | |
| 2 | human | 2957 | GTF2A1 | general transcription facto... | NM_201595.3 | 89.6% | 89.6% | 0_1ins117 |
| 3 | human | 2957 | GTF2A1 | general transcription facto... | NM_001278940.2 | 86.7% | 86.7% | 0_1ins150 |
| 4 | mouse | 83602 | Gtf2a1 | general transcription facto... | NM_031391.2 | 90.3% | 96.5% | (many diffs) |
| 5 | mouse | 83602 | Gtf2a1 | general transcription facto... | NM_175335.3 | 80.9% | 86.2% | (many diffs) |
| 6 | mouse | 83602 | Gtf2a1 | general transcription facto... | XM_017315260.1 | 78.3% | 83.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1197
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgaactcg gcaaatacaa acaccgtgcc taaattatac agatctgtga 121 ttgaagatgt cattaatgat gtgagagaca tctttctgga tgatggagtg gatgaacaag 181 tactgatgga actaaaaact ttatgggaaa acaaactaat gcagtccagg gcagtagatg 241 gatttcattc agaagagcag cagcttctac tgcaagttca acagcagcat caaccccagc 301 agcagcagca tcaccaccat caccatcatc agcaagctca gcctcagcag acagtacctc 361 agcaagcgca gacccagcag gttcttattc ctgcatcaca gcaagccaca gcaccacaag 421 ttattgttcc agattctaag ttgatacagc atatgaatgc atcaaacatg agtgctgctg 481 ctacagctgc taccttagca ctccctgcag gtgtgactcc tgttcagcag atattaacaa 541 attcaggcca gcttcttcag gtggtcagag cagccaatgg tgcccaatat atctttcagc 601 ctcagcagtc agtggttcta caacaacagg ttataccaca aatgcagcct ggtggagtac 661 aagctcctgt tatacagcag gtgctggctc ctcttcctgg agggatttca ccacagacag 721 gtgtcatcat ccagcctcag caaatcttat ttacaggaaa taagactcaa gttataccta 781 cgacagtggc agcaccTACA CCAGCCCAAG CACAGATAAC TGCAACTGGC CAGCAGCAAC 841 CGCAGGCCCA GCCTGCTCAA ACACAAGCTC CATTGGTCTT ACAAGTTGAT GGAACTGGGG 901 ATACATCATC TGAAGAAGAT GAAGATGAAG AAGAAGACTA TGATGATGAT GAGGAGGAAG 961 ACAAAGAGAA AGATGGAGCT GAAGATGGGC AGGTGGAAGA AGAGCCCCTC AATAGTGAAG 1021 ATGATGTGAG TGATGAGGAA GGACAGGAAC TCTTTGACAC AGAAAATGTT GTTGTATGCC 1081 AATATGATAA GATACACAGA AGTAAAAACA AATGGAAATT TCATCTCAAG GATGGCATTA 1141 TGAATCTTAA TGGAAGAGAT TATATATTTT CCAAAGCCAT TGGAGATGCA GAATGGTTGC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GAAGTCAGTT CTCCTAAGGA CCGCTCACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt