Transcript: Mouse NM_175335.3

Mus musculus general transcription factor II A, 1 (Gtf2a1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Gtf2a1 (83602)
Length:
5691
CDS:
190..1209

Additional Resources:

NCBI RefSeq record:
NM_175335.3
NBCI Gene record:
Gtf2a1 (83602)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_175335.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000082282 GTTCAACAGCTACTAACAAAT pLKO.1 532 CDS 100% 13.200 10.560 N Gtf2a1 n/a
2 TRCN0000218912 TCATCTCAAGGATGGCATTAT pLKO_005 1131 CDS 100% 13.200 9.240 N GTF2A1 n/a
3 TRCN0000424004 TGTCGTCTGCCAGTATGATAA pLKO_005 1080 CDS 100% 13.200 9.240 N Gtf2a1 n/a
4 TRCN0000082278 CTCCGGTTCAACAGCTACTAA pLKO.1 527 CDS 100% 5.625 3.938 N Gtf2a1 n/a
5 TRCN0000082280 ACTCCGGTTCAACAGCTACTA pLKO.1 526 CDS 100% 4.950 3.465 N Gtf2a1 n/a
6 TRCN0000434267 AGCAAGCGCAACCTCAGCAAA pLKO_005 338 CDS 100% 4.950 3.465 N Gtf2a1 n/a
7 TRCN0000082281 GATGACGGAGTGGATGAACAA pLKO.1 163 5UTR 100% 4.950 2.970 N Gtf2a1 n/a
8 TRCN0000093082 GAAGATGAAGATGAAGAAGAA pLKO.1 925 CDS 100% 4.950 2.475 Y Gm5518 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_175335.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00705 pDONR223 100% 80.9% 86.2% None (many diffs) n/a
2 ccsbBroad304_00705 pLX_304 0% 80.9% 86.2% V5 (many diffs) n/a
3 TRCN0000479009 AGTCAGTTCTCCTAAGGACCGCTC pLX_317 35.7% 80.9% 86.2% V5 (many diffs) n/a
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