Construct: ORF TRCN0000479023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008257.1_s317c1
- Derived from:
- ccsbBroadEn_01783
- DNA Barcode:
- CTTGATAATCGTGAGACTTATGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XPA (7507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479023
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7507 | XPA | XPA, DNA damage recognition... | NM_000380.3 | 100% | 100% | |
| 2 | human | 7507 | XPA | XPA, DNA damage recognition... | XM_006717278.1 | 95.3% | 94.8% | (many diffs) |
| 3 | human | 7507 | XPA | XPA, DNA damage recognition... | NM_001354975.1 | 81.2% | 79.8% | (many diffs) |
| 4 | human | 7507 | XPA | XPA, DNA damage recognition... | NR_149092.1 | 48.5% | 1_117del;397_398ins106;831_1363del | |
| 5 | human | 7507 | XPA | XPA, DNA damage recognition... | NR_027302.1 | 47.5% | 1_117del;788_1018del;1168_1722del | |
| 6 | human | 7507 | XPA | XPA, DNA damage recognition... | NR_149093.1 | 43.3% | 1_117del;789_1208del;1357_1889del | |
| 7 | human | 7507 | XPA | XPA, DNA damage recognition... | NR_149094.1 | 37.7% | (many diffs) | |
| 8 | human | 7507 | XPA | XPA, DNA damage recognition... | NR_149091.1 | 37.2% | 1_117del;397_398ins272;665_1197del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaaa 61 ttggcatggc ggcggccgac ggggctttgc cggaggcggc ggctttagag caacccgcgg 121 agctgcctgc ctcggtgcgg gcgagtatcg agcggaagcg gcagcgggca ctgatgctgc 181 gccaggcccg gctggctgcc cggccctact cggcgacggc ggctgcggct actggaggca 241 tggctaatgt aaaagcagcc ccaaagataa ttgacacagg aggaggcttc attttagaag 301 aggaagaaga agaagaacag aaaattggaa aagttgttca tcaaccagga cctgttatgg 361 AATTTGATTA TGTAATATGC GAAGAATGTG GGAAAGAATT TATGGATTCT TATCTTATGA 421 ACCACTTTGA TTTGCCAACT TGTGATAACT GCAGAGATGC TGATGATAAA CACAAGCTTA 481 TAACCAAAAC AGAGGCAAAA CAAGAATATC TTCTGAAAGA CTGTGATTTA GAAAAAAGAG 541 AGCCACCTCT TAAATTTATT GTGAAGAAGA ATCCACATCA TTCACAATGG GGTGATATGA 601 AACTCTACTT AAAGTTACAG ATTGTGAAGA GGTCTCTTGA AGTTTGGGGT AGTCAAGAAG 661 CATTAGAAGA AGCAAAGGAA GTCCGACAGG AAAACCGAGA AAAAATGAAA CAGAAGAAAT 721 TTGATAAAAA AGTAAAAGAA TTGCGGCGAG CAGTAAGAAG CAGCGTGTGG AAAAGGGAGA 781 CGATTGTTCA TCAACATGAG TATGGACCAG AAGAAAACCT AGAAGATGAC ATGTACCGTA 841 AGACTTGTAC TATGTGTGGC CATGAACTGA CATATGAAAA AATGTGCCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGCCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA CTTGATAATC GTGAGACTTA TGACACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt