Construct: ORF TRCN0000479037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002253.1_s317c1
- Derived from:
- ccsbBroadEn_08904
- DNA Barcode:
- TTTTGATAGCTGTGACGACCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIR (79002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79002 | TRIR | telomerase RNA component in... | NM_024038.4 | 99.8% | 99.4% | 11G>A |
| 2 | human | 79002 | TRIR | telomerase RNA component in... | NM_001329738.2 | 85% | 84.6% | 11G>A;345_346ins78 |
| 3 | human | 79002 | TRIR | telomerase RNA component in... | NM_001329739.2 | 74.8% | 63.7% | 11G>A;322_323ins23;396_397ins109 |
| 4 | human | 79002 | TRIR | telomerase RNA component in... | NR_138095.2 | 51.9% | (many diffs) | |
| 5 | mouse | 68544 | Trir | telomerase RNA component in... | NM_026760.2 | 89.6% | 90.9% | (many diffs) |
| 6 | mouse | 68544 | Trir | telomerase RNA component in... | XR_387774.3 | 53.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcccaaggg agacgggcgg agcctcaggg ccgggaggct ccgggccccg 121 cgggcggtgg cggtggcggg agccgttggg ctgagtcggg atcggggacg tcgcccgaga 181 gcggggacga ggaggtgTCG GGCGCGGGTT CGAGCCCGGT GTCGGGCGGC GTGAACTTGT 241 TCGCCAACGA CGGCAGCTTC CTGGAGCTGT TCAAGCGGAA GATGGAGGAG GAGCAGCGGC 301 AGCGGCAGGA GGAGCCGCCC CCGGGTCCGC AGCGACCCGA CCAGTCGGCC GCCGCCGCTG 361 GCCCCGGGGA TCCGAAGAGG AAGGGCGGTC CGGGCTCCAC ACTTAGCTTC GTGGGCAAAC 421 GCAGAGGCGG GAACAAACTA GCCCTCAAGA CGGGAATAGT AGCCAAGAAG CAGAAGACGG 481 AGGATGAGGT ATTAACAAGT AAAGGTGACG CGTGGGCCAA GTACATGGCA GAAGTGAAAA 541 AGTACAAAGC TCACCAGTGC GGTGACGATG ATAAAACTCG GCCCCTGGTG AAATGCCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAT TTTGATAGCT GTGACGACCT TCAACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att