Construct: ORF TRCN0000479042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016091.1_s317c1
- Derived from:
- ccsbBroadEn_13987
- DNA Barcode:
- CTGCGGCATTCATGGACTTGGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A16 (8034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8034 | SLC25A16 | solute carrier family 25 me... | NM_152707.4 | 100% | 100% | |
| 2 | human | 8034 | SLC25A16 | solute carrier family 25 me... | NM_001324312.2 | 89.5% | 83.6% | 843_870del;945_946ins79 |
| 3 | human | 8034 | SLC25A16 | solute carrier family 25 me... | NM_001324313.2 | 84.5% | 84.6% | 843_843delGins154 |
| 4 | human | 8034 | SLC25A16 | solute carrier family 25 me... | NM_001324314.2 | 70.4% | 70.4% | 0_1ins294 |
| 5 | human | 8034 | SLC25A16 | solute carrier family 25 me... | NM_001324315.1 | 70.4% | 70.4% | 0_1ins294 |
| 6 | human | 8034 | SLC25A16 | solute carrier family 25 me... | NM_001324317.2 | 59.6% | 59.6% | 0_1ins402 |
| 7 | human | 8034 | SLC25A16 | solute carrier family 25 me... | NR_136737.1 | 26.1% | (many diffs) | |
| 8 | mouse | 73132 | Slc25a16 | solute carrier family 25 (m... | NM_175194.2 | 86.6% | 92.7% | (many diffs) |
| 9 | mouse | 73132 | Slc25a16 | solute carrier family 25 (m... | XM_017314118.1 | 50% | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcgacg gccgcggcag ccctggcggc ggccgatccc cctcccgcaa 121 tgccgcaggc ggcaggggcc ggagggccca caacccgcag agacttctac tggctgcgct 181 cctttctggc cggaggtatt gctggatgct gtgccaaaac aacagttgct ccattggatc 241 gagtaaaggt tttattacaa gctcacaatc accattacaa gcatttagga gtattttctg 301 cattgcgtgc tgttcctcaa aaagaaggat tccttggatt gtataaagga aatggtgcaa 361 tgatgattcg aatctttccc tatggtgcaa tccagtttat ggcatttgag cattataaaa 421 cgttaattac tacgaagctg ggaatttcag gtcatgtgca cagattaatg gctggatcca 481 tggcaggtat gacagcagtt atctgtactt accctcttga catggttagg gtccgcctag 541 cattccaggt gaaaggggaa cacagctata caggaattat tcatgctttc aaaacaattt 601 atgcaaagga aggtggtttc tttggatttt acagaggtct gatgcctACT ATTTTAGGAA 661 TGGCTCCATA TGCAGGTGTT TCATTTTTTA CTTTTGGTAC CTTGAAGAGT GTTGGGCTTT 721 CCCATGCTCC TACCCTTCTT GGCAGACCTT CATCAGACAA TCCTAATGTC TTAGTTTTGA 781 AAACTCATGT AAACTTACTT TGTGGTGGTG TTGCTGGAGC AATAGCGCAG ACAATATCCT 841 ACCCATTTGA TGTGACTCGT CGGCGAATGC AATTAGGAAC TGTTCTGCCG GAATTTGAAA 901 AGTGCCTTAC CATGCGGGAT ACTATGAAGT ATGTCTATGG ACACCATGGA ATTCGAAAAG 961 GACTCTATCG TGGTTTATCT CTTAATTACA TTCGCTGTAT TCCCTCTCAA GCAGTGGCTT 1021 TTACAACATA CGAACTTATG AAGCAGTTTT TTCACCTCAA CTACCCAACT TTCTTGTACA 1081 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1141 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1201 AGGACGACTG CGGCATTCAT GGACTTGGAG AACGCGTTAA GTCgacaatc aacctctgga 1261 ttacaaaatt tgtgaaagat t