Construct: ORF TRCN0000479059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018143.1_s317c1
- Derived from:
- ccsbBroadEn_00994
- DNA Barcode:
- GGATACACTCTCTCCACATCCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEA1 (4201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4201 | MEA1 | male-enhanced antigen 1 | NM_014623.4 | 100% | 100% | |
| 2 | human | 4201 | MEA1 | male-enhanced antigen 1 | NM_001363578.1 | 95.6% | 94.2% | (many diffs) |
| 3 | human | 4201 | MEA1 | male-enhanced antigen 1 | NM_001318942.1 | 92.9% | 92.9% | 0_1ins39 |
| 4 | human | 4201 | MEA1 | male-enhanced antigen 1 | NM_001318943.1 | 92.9% | 92.9% | 0_1ins39 |
| 5 | human | 4201 | MEA1 | male-enhanced antigen 1 | XM_024446445.1 | 92.9% | 92.9% | 0_1ins39 |
| 6 | human | 4201 | MEA1 | male-enhanced antigen 1 | XM_017010868.1 | 74% | 65.4% | (many diffs) |
| 7 | mouse | 17256 | Mea1 | male enhanced antigen 1 | NM_001277310.1 | 84.3% | 86.6% | (many diffs) |
| 8 | mouse | 17256 | Mea1 | male enhanced antigen 1 | NM_001277311.1 | 84.3% | 86.6% | (many diffs) |
| 9 | mouse | 17256 | Mea1 | male enhanced antigen 1 | NM_001277312.1 | 84.3% | 86.6% | (many diffs) |
| 10 | mouse | 17256 | Mea1 | male enhanced antigen 1 | NM_010787.2 | 84.3% | 86.6% | (many diffs) |
| 11 | mouse | 17256 | Mea1 | male enhanced antigen 1 | XM_006523748.3 | 84.3% | 86.6% | (many diffs) |
| 12 | mouse | 17256 | Mea1 | male enhanced antigen 1 | XM_006523749.3 | 84.3% | 86.6% | (many diffs) |
| 13 | mouse | 17256 | Mea1 | male enhanced antigen 1 | XM_006523750.3 | 84.3% | 86.6% | (many diffs) |
| 14 | mouse | 17256 | Mea1 | male enhanced antigen 1 | NM_001277309.1 | 78.4% | 76.3% | (many diffs) |
| 15 | mouse | 17256 | Mea1 | male enhanced antigen 1 | XM_006523746.3 | 70.3% | 71.5% | (many diffs) |
| 16 | mouse | 17256 | Mea1 | male enhanced antigen 1 | NR_102392.1 | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcctgaaagg catctgtcag gcgcccctgc ccggatggca acagtagttc 121 taggaggaga caccatgggc cctgagcgta tcttccccaa tcagactgag gaactgggac 181 atcagggccc ttcagaaggc actggggatt ggagcagtga ggagccTGAG GAAGAGCAGG 241 AGGAAACGGG GTCGGGCCCA GCTGGCTACT CCTACCAGCC CCTGAACCAA GATCCTGAAC 301 AAGAGGAGGT GGAACTGGCA CCAGTGGGGG ATGGAGATGT AGTTGCTGAC ATCCAGGATC 361 GAATCCAGGC CCTGGGGCTT CATTTGCCAG ACCCACCATT AGAGAGTGAA GATGAAGATG 421 AGGAGGGAGC TACAGCGTTG AACAACCACA GCTCTATTCC CATGGACCCA GAACATGTAG 481 AGCTGGTGAA AAGGACAATG GCTGGAGTAA GCCTGCCTGC GCCAGGGGTT CCTGCCTGGG 541 CTCGGGAGAT ATCGGATGCC CAGTGGGAAG ATGTGGTACA GAAAGCCCTC CAAGCCCGGC 601 AGGCATCCCC TGCCTGGAAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAT ACACTCTCTC 781 CACATCCCTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt