Construct: ORF TRCN0000479060
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018121.2_s317c1
- Derived from:
- ccsbBroadEn_09306
- DNA Barcode:
- ATCGTACTTTTTCCCCTATTTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCEAL8 (90843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479060
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90843 | TCEAL8 | transcription elongation fa... | NM_001006684.1 | 99.7% | 99.1% | 340T>C |
| 2 | human | 90843 | TCEAL8 | transcription elongation fa... | NM_153333.3 | 99.7% | 99.1% | 340T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 441
- ORF length:
- 351
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCAAAAGTC TTGTGAAGAA AATGAGGGAA 121 AACCACAGAA CATGCCAAAG GCCGAGGAAG ATCGCCCTTT GGAGGATGTA CCACAGGAGG 181 CAGAAGGAAA TCCTCAACCT TCCGAAGAAG GCGTAAGCCA GGAAGCAGAA GGAAACCCCA 241 GAGGAGGGCC GAATCAGCCT GGCCAGGGAT TTAAAGAGGA CACACCCGTT AGGCATTTGG 301 ACCCTGAAGA AATGATAAGA GGAGTAGATG AGCTTGAAAG GCTTAGGGAA GAGATAAGAA 361 GAGTAAGAAA CAAGTTTGTG ATGATGCATT GGAAGCAAAG ACATTCACGC AGCCGTCCTT 421 ATCCTGTGCG CTTTAGGCCT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATCG TACTTTTTCC 601 CCTATTTAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt