Construct: ORF TRCN0000479069
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003108.1_s317c1
- Derived from:
- ccsbBroadEn_12509
- DNA Barcode:
- TTATCAGGCATCTTTTTACTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPBP1 (65056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479069
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001203246.1 | 59.9% | 59.9% | 1_363del |
| 2 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001127235.2 | 38.9% | 38.9% | 1_852del |
| 3 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_022913.4 | 38.2% | 38.2% | 1_876del |
| 4 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001127236.2 | 37.7% | 37.7% | 1_897del |
| 5 | human | 65056 | GPBP1 | GC-rich promoter binding pr... | NM_001331037.1 | 36.7% | 36.7% | 1_936del |
| 6 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_017315613.1 | 55.7% | 58.2% | (many diffs) |
| 7 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_017315614.1 | 55.7% | 58.2% | (many diffs) |
| 8 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_006517775.3 | 48.3% | 50.5% | (many diffs) |
| 9 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_006517773.3 | 45.7% | 47.8% | (many diffs) |
| 10 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | NM_001122963.1 | 35.5% | 37.2% | (many diffs) |
| 11 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | NM_028487.4 | 34.1% | 35.6% | (many diffs) |
| 12 | mouse | 73274 | Gpbp1 | GC-rich promoter binding pr... | XM_006517772.1 | 34.1% | 35.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg cactgataag aagagtgaat ttttgaaagc attgaaaaga gacagagtag 121 aagaggaaca tgaagatgaa agccgtgctg gctcagagaa ggatgacgac tcatttaatt 181 tacataacag caatagtact caccaagaaa gggatataaa ccgaaacttc gatgaaaatg 241 aaattcctca agagaatggc aatgcctcag tgatttccca gcagatcatt cggTCTTCAA 301 CCTTCCCACA AACTGATGTT CTTTCAAGTT CACTTGAGGC AGAACACAGA TTGTTAAAGG 361 AAATGGGCTG GCAGGAAGAC AGTGAAAATG ATGAAACATG TGCTCCCTTA ACTGAGGATG 421 AAATGAGAGA ATTCCAAGTT ATTAGTGAAC AGTTACAGAA GAATGGTCTG AGAAAAAATG 481 GTATTTTGAA AAATGGCTTG ATCTGTGACT TCAAGTTTGG ACCGTGGAAG AACAGCACTT 541 TCAAACCCAC AACTGAGAAT GATGACACAG AGACAAGTAG CAGTGATACA TCAGATGACG 601 ACGATGTGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTATCA GGCATCTTTT TACTTATTAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt