Construct: ORF TRCN0000479115
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005038.1_s317c1
- Derived from:
- ccsbBroadEn_13125
- DNA Barcode:
- TTGGGTACTTATATCCACCCCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRIQ3 (127255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479115
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | XM_024453191.1 | 94.6% | 93.2% | (many diffs) |
| 2 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | XM_024453192.1 | 93.2% | 93.6% | (many diffs) |
| 3 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | XM_024453188.1 | 72.2% | 71.1% | (many diffs) |
| 4 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | XM_017000287.2 | 63.1% | 62.1% | (many diffs) |
| 5 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | XM_024453185.1 | 59.1% | 58.1% | (many diffs) |
| 6 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | NM_001322315.2 | 45.4% | 44.6% | (many diffs) |
| 7 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | NM_001105659.2 | 31.3% | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcatggaaca gtcacagaag agctaaccag tcatgaagaa tggagtcact 121 ataatgaaaa cataagagaa ggtcaaaaag attttgtttt tgtgaagttc aatggccttc 181 atttaaagtc tatggaaaat ttgcagTCTT GCATCTCTCT TAGAGTATGC ATCTTCTCAA 241 ACAATTTTAT TACAGATATT CATCCACTTC AAAGTTGTAT AAAATTAATC AAACTTGATC 301 TCCATGGAAA TCAGATAAAG AGTCTACCAA ATACCAAATT TTGGAATGGA TTGAAGAACC 361 TAAAACTACT CTATCTTCAT GACAATGGGT TTGCAAAGTT AAAGAATATA TGTGTATTAT 421 CTGCCTGTCC AACCCTCATT GCCCTCACTA TGTTTGATTG TCCAGTAAGC CTTAAAAAAG 481 GATATAGACA TGTTCTTGTT AACAGTATAT GGCCTCTCAA AGCGCTGGAT CATCATGTGA 541 TTTCTGATGA AGAAATAATT CAGAACTGGC ATCTTCCTGA AAGATTCAAA GCATGTAACC 601 ATCGACTTTT CTTTAATTTC TGCCCAGCTT TGAGAAAGGA GAAAATGAAG CATAGTGAGG 661 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGATT GGGTACTTAT ATCCACCCCC ATACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt