Construct: ORF TRCN0000479129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002396.2_s317c1
- Derived from:
- ccsbBroadEn_11671
- DNA Barcode:
- ACGATACTGGAACCGGAGAAGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERC1 (23085)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479129
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | NR_027949.3 | 3.2% | 1_4983del;5110C>G;5257_8485del | |
| 2 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | XR_001748635.1 | 2.9% | 1_5784del;5911C>G;6058_9286del | |
| 3 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | NR_027946.3 | 2.9% | 1_5832del;5959C>G;6106_9334del | |
| 4 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | XR_001748637.1 | 2.9% | 1_5876del;6003C>G;6150_9378del | |
| 5 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | XR_001748636.1 | 2.8% | 1_5885del;6012C>G;6159_9387del | |
| 6 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | NR_027948.3 | 2.8% | 1_5964del;6091C>G;6238_9466del | |
| 7 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | XR_001748633.1 | 2.8% | 1_6005del;6132C>G;6279_9507del | |
| 8 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | XR_001748632.1 | 2.8% | 1_6017del;6144C>G;6291_9519del | |
| 9 | human | 23085 | ERC1 | ELKS/RAB6-interacting/CAST ... | XR_001748634.1 | 2.8% | 1_6113del;6240C>G;6387_9615del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 363
- ORF length:
- 273
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCTAAACA AGTGGTCTGG CACGGGCACT 121 GGCCAGCTGC TCTCTCCAAG CAGGTGGCCC AGATCCCACC CACGTGGACT TTCTCATCAG 181 GTGCAGCGCC TGCCACTCTC AGCCACTGGG TGTGTGACTC TCCTCTTCAT CTCAGCATTC 241 TCCATCACTT CCCCTCCAGA AAAACGGATG GAAGGAAGCC CTCTGTGACA CTGCTTCTGA 301 GAAGAAGCAT TTCCGGGACC GATATCATCT GTCTGGTCTC TGTGAACAGC AAGGAATCTT 361 CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GATACTGGAA CCGGAGAAGT TAACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt