Construct: ORF TRCN0000479158
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004528.1_s317c1
- Derived from:
- ccsbBroadEn_05406
- DNA Barcode:
- AGTCATGGAACATCCTTTATACGT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SREK1IP1 (285672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479158
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285672 | SREK1IP1 | SREK1 interacting protein 1 | NM_173829.4 | 99.7% | 82.9% | 359delA |
| 2 | human | 285672 | SREK1IP1 | SREK1 interacting protein 1 | XM_011543344.3 | 89.4% | 72.1% | 13_14ins48;311delA |
| 3 | mouse | 67288 | Srek1ip1 | splicing regulatory glutami... | NM_026075.3 | 85.5% | 77.7% | (many diffs) |
| 4 | mouse | 67288 | Srek1ip1 | splicing regulatory glutami... | NR_135272.1 | 20.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 534
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagtccca ggttgcaaca aggacagtgt cagagcaggc tgtaaaaaat 121 gtggctaccc tggtcacctg acttttgaat gccgcaattt tctccgagta gaccctaaaa 181 gggacatagt tttggatgtc agcagtacaa gtagtgaaga tagcgatgaa gagaatgaag 241 aactgaataa attgcagGCA TTACAGGAAA AAAGAATAAA TGAAGAAGAG GAAAAGAAGA 301 AAGAAAAAAG CAAAGAAAAA ATCAAATTGA AAAAAAAAAG GAAAAGGTCT TACTCATCCA 361 GTTCCACTGA AGAGGACACT TCAAAACAAA AGAAACAAAA ATATCAGAAG AAAGAAAAGA 421 AAAAAGAAAA AAGAGTAAAT CAAAAAAAGG GAAACATCAC AAAAAGGAAA AAAAGAAGAG 481 AAAAAAGGAA AAGCATTCTT CTACACCTAA TAGTTCTGAA TTCTCCAGAA AGTTGCCAAC 541 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 601 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 661 ATCTTGTGGA AAGGACGAAG TCATGGAACA TCCTTTATAC GTACGCGTTA AGTCgacaat 721 caacctctgg attacaaaat ttgtgaaaga tt