Construct: ORF TRCN0000479174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014903.1_s317c1
- Derived from:
- ccsbBroadEn_07502
- DNA Barcode:
- AGTTAAGCCATTGAGTCCCAAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POM121 (9883)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479174
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9883 | POM121 | POM121 transmembrane nucleo... | NM_172020.4 | 99.9% | 100% | 321G>C |
| 2 | human | 9883 | POM121 | POM121 transmembrane nucleo... | XM_006716196.3 | 99.9% | 100% | 321G>C |
| 3 | human | 9883 | POM121 | POM121 transmembrane nucleo... | XM_017012853.2 | 99.9% | 100% | 321G>C |
| 4 | human | 9883 | POM121 | POM121 transmembrane nucleo... | XM_017012854.2 | 99.9% | 100% | 321G>C |
| 5 | human | 100101267 | POM121C | POM121 transmembrane nucleo... | NM_001099415.3 | 98.3% | 97.2% | (many diffs) |
| 6 | human | 9883 | POM121 | POM121 transmembrane nucleo... | NM_001257190.3 | 96.6% | 96.2% | (many diffs) |
| 7 | human | 9883 | POM121 | POM121 transmembrane nucleo... | NM_001367610.1 | 93.9% | 94% | 321G>C;870_871ins177 |
| 8 | human | 9883 | POM121 | POM121 transmembrane nucleo... | XM_005250726.4 | 78.7% | 78.7% | 1_795del;1116G>C |
| 9 | human | 9883 | POM121 | POM121 transmembrane nucleo... | XM_005250727.4 | 74% | 74% | 1_795del;1116G>C;1665_1666ins177 |
| 10 | human | 9883 | POM121 | POM121 transmembrane nucleo... | XM_011516730.2 | 71% | 71% | 1_795del;1116G>C;1274_1275ins288 |
| 11 | human | 22932 | POMZP3 | POM121 and ZP3 fusion | NM_152992.2 | 10% | 8.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3018
- ORF length:
- 2952
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gtgtagccca gtgactgtga ggatcgcccc tcctgacaga agattttcgc 121 gttctgcgat accagagcag ataatcagct caacactgtc ctcaccatca agtaacgccc 181 cagacccatg tgcaaaggag acagtactga gtgccctcaa agagaaggag aagaaaagga 241 cagtggagga agaagaccaa atattccttg atggccagga aaataaaaga aggcgccatg 301 atagcagtgg cagtggacat tcagcatttg agcccctggt ggccaatgga gtccccgctt 361 cttttgtgcc taagcctggg tctctcaaga gaggcctcaa ttctcagagc tcagatgacc 421 acttgaataa gagatcccga agctcttcca tgagctcctt gacaggcgct tacgcaagtg 481 gcatccctag ctccagccgc aatgccatta ccagttccta cagctccact cgaggcatct 541 cacagctctg gaagagaaat ggccccagtt catcaccctt ctctagccca gcctcctccc 601 gctcccagac accggagagg ccagcaaaga aaataagaga agaggagctg tgtcatcatt 661 ccagttcttc aactccattg gcagcagaca gggagtccca gggagaaaag gctgcagata 721 caaccccaag gaagaaacaa aactcgaatt ctcagtctac acctggcagc tctgggcagc 781 gtaagcggaa agttcagctg ctgccttctc ggcgagggga acagctgacc ttgcctccac 841 ctccccagct tggctattcg atcactgccg aggacctaga cttagagaag aaggcttcat 901 tacagtggtt caaccaggcc ttggaggaca agagcgatgc tgcctcgaac tctgtcactg 961 agaccccacc tatcactcag ccttcattta cctttaccct gcctgctgct gcacctgcct 1021 ccccacccac ctccctcctg gccccaagca ccaacccact gttagagagc ttgaagaaga 1081 tgcagactcc cccgagcctg ccaccctgcc cagaatctgc tggagcagca accactgagg 1141 ccctctcacc tccaaagaca cccagcctcc tacccccgct gggtttatca cagtcagggc 1201 cgccagggct gctccccagc ccctcctttg actccaaacc cccgaccact ttgctggggc 1261 tgatccctgc tccatccatg gtaccagcca ctgacaccaa ggcacctcca acccttcagg 1321 cagagacggc taccaaaccc caagccacat ctgccccgtc ccccgccccc aagcaaagct 1381 tcctgtttgg aacacagaac acctcacctt ccagccctgc cgcccctgct gcatcttcag 1441 cacctcccat gttcaagccc attttcacgg ctccacccaa gagtgagaag gaaggcccca 1501 caccgcctgg cccttcagtc acagccacag cgccctccag ctcctccctc cccacgacca 1561 ccagcaccac agccccgacc ttccagcctg tctttagcag catggggcca cctgcatctg 1621 tgcccttgcc tgctcccttc ttcaagcaga caactactcc cgccactgct cccaccacaa 1681 ctgccccgct cttcactggc ctggccagcg ccacctctgc tgtggctccc atcacctctg 1741 ccagtccatc cacagactct gcttcgaagc ctgcgtttgg ctttggcata aacagtgtga 1801 gcagcagcag tgtgagtacc acgaccagca ccgccactgc cgcctcacag cctttcctct 1861 tcggggcgcc ccaggcctct gctgccagct tcaccccggc catgggctcc atattccagt 1921 ttggcaaacc tcctgccttg cccacaacca ccacagtcac caccttcagc cagtccctgc 1981 acactgccgt gccaacggcc accagcagca gcgctgccga ctttagtggt tttggcagca 2041 ccctcgccac ctccgccccg gccaccagca gccagcccac tctgacgttc agtaacacga 2101 gcacccccac gttcaacatt ccctttggct caagcgccaa gtccccgctc ccatcatatc 2161 cgggagccaa cccccagccc gcatttgggg ccgctgaggg gcagccaccg ggggccgcca 2221 agccggccct tgcccccagc tttggcagct ctttcacttt tggaaactct gcagccccgg 2281 ctgctgcacc cacacctgca cctccgtcca tgatcaaggt cgtgcctgcg tacgtgccta 2341 cgcccatcca tcctatcttt ggcggtgcca cgcactcggc gtttgggttg aaagccacgg 2401 cttcggcctt cggcgctccc gccagctcac agcccgcctt tggcggctcc actgctgtct 2461 tcttcggtgc agccaccagc tccggctttg gagccaccac ccagaccgcc agcagcggga 2521 gcagcagctc ggtgtttggc agcacaacac catcaccctt cacgtttggg ggttcggcag 2581 cccccgctgg cagtgggagc tttgggatca atgtggccac cccaggctcc agcaccacca 2641 ccggagcttt cagctttgga gcaggacaga gtgggagcac agccacctcc acccccTTCG 2701 CAGGGGGCTT AGGTCAGAAC GCCCTGGGCA CCACCGGCCA GAGCACACCG TTTGCCTTCA 2761 ACGTGAGCAG CACAACTGAG AGCAAACCTG TGTTTGGAGG CACCGCCACC CCCACCTTTG 2821 GTCTGAACAC CCCTGCGCCT GGAGTGGGCA CATCAGGCAG CAGCCTCTCC TTTGGGGCAT 2881 CCTCAGCACC CGCCCAAGGC TTTGTTGGTG TTGCACCTTT CGGATCGGCG GCCCTTTCAT 2941 TTTCCATTGG TGCGGGATCC AAGACCCCAG GGGCTCGACA GCGACTGCAG GCCCGAAGGC 3001 AGCACACCCG CAAAAAGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3061 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3121 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGTTAAG CCATTGAGTC 3181 CCAAACCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt