Transcript: Human XM_005250727.4

PREDICTED: Homo sapiens POM121 transmembrane nucleoporin (POM121), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
POM121 (9883)
Length:
6639
CDS:
1006..4578

Additional Resources:

NCBI RefSeq record:
XM_005250727.4
NBCI Gene record:
POM121 (9883)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005250727.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416775 GGGCTATCCGTAAAGCTTTAT pLKO_005 4998 3UTR 100% 13.200 9.240 N POM121 n/a
2 TRCN0000444257 ACTCGGCGTTTGGGTTGAAAG pLKO_005 3932 CDS 100% 10.800 6.480 N POM121 n/a
3 TRCN0000060071 CCATGTGCAAAGGAGACAGTA pLKO.1 1921 CDS 100% 4.950 2.970 N POM121 n/a
4 TRCN0000156699 CTGCGATACCAGAGCAGATAA pLKO.1 1859 CDS 100% 13.200 6.600 Y POMZP3 n/a
5 TRCN0000255860 GCGATACCAGAGCAGATAATC pLKO_005 1861 CDS 100% 13.200 6.600 Y POM121C n/a
6 TRCN0000255858 GCGTTTGGCTTTGGCATAAAC pLKO_005 3331 CDS 100% 13.200 6.600 Y POM121C n/a
7 TRCN0000438142 GGTGTGCACCTGATGGGATTT pLKO_005 4970 3UTR 100% 10.800 5.400 Y POM121 n/a
8 TRCN0000060069 CCAGTTCATCACCCTTCTCTA pLKO.1 2300 CDS 100% 4.950 2.475 Y POM121 n/a
9 TRCN0000154080 CAGAGCAGATAATCAGCTCAA pLKO.1 1868 CDS 100% 4.050 2.025 Y POMZP3 n/a
10 TRCN0000060068 CCCACGTTCAACATTCCCTTT pLKO.1 3664 CDS 100% 4.050 2.025 Y POM121 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 87 5UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 87 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005250727.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07502 pDONR223 100% 74% 74% None 1_795del;1116G>C;1665_1666ins177 n/a
2 ccsbBroad304_07502 pLX_304 0% 74% 74% V5 1_795del;1116G>C;1665_1666ins177 n/a
3 TRCN0000479174 AGTTAAGCCATTGAGTCCCAAACC pLX_317 11.1% 74% 74% V5 1_795del;1116G>C;1665_1666ins177 n/a
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