Construct: ORF TRCN0000479175
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014721.1_s317c1
- Derived from:
- ccsbBroadEn_12986
- DNA Barcode:
- TCCCGATCGGGCTCTAACACCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZBTB47 (92999)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479175
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92999 | ZBTB47 | zinc finger and BTB domain ... | NM_145166.4 | 49.6% | 49.6% | 1_1128del |
| 2 | mouse | 270210 | Zfp651 | zinc finger protein 651 | XM_006512145.3 | 40.1% | 41.9% | (many diffs) |
| 3 | mouse | 270210 | Zfp651 | zinc finger protein 651 | NM_001166644.1 | 38.7% | 40.3% | (many diffs) |
| 4 | mouse | 270210 | Zfp651 | zinc finger protein 651 | XM_006512146.1 | 23.1% | 19.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1179
- ORF length:
- 1113
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacacggtcc cgggagaacg cccggcgccg gggtacccct gaacctgaag 121 aagctgggcg gcggggtggg aagaggccaa agccaccccc tggagtggcc tctgcatcgg 181 cccgagggcc gccagccact gatgggctgg gggccaaggt gaagctggag gagaagcagc 241 accatccatg ccagaagtgc ccacgagttt tcaacaaccg ctggtacctg gagaaacaca 301 tgaatgtgac ccacagccgc atgcagatct gcgaccagtg cggcaagcgc ttcctgctgg 361 agagcgagct gctgctgcac aggcagacag actgcgagcg caacatccag tgtgtgacat 421 gtggcaaagc tttcaagaag ctttggtccc tccatgagca taacaagatt gtgcacggct 481 acgcagagaa gaagttctca tgcgagatct gtgagaagaa gttctacacc atggcccacg 541 tgcgtaagca catggttgcc cacaccaagg acatgccctt cacctgcgag acctgcggaa 601 agtccttcaa gcgcagcatg tccctcaagg tgcactcact gcagcactca ggggagaagc 661 cgttcagatg tgagaactgc aatgagcgct tccagtacaa gtaccagctg cggtcacaca 721 tgagcatcca cattGGCCAC AAGCAGTTCA TGTGCCAATG GTGCGGCAAG GACTTCAACA 781 TGAAGCAGTA CTTCGATGAG CACATGAAGA CCCACACAGG AGAGAAGCCG TACATCTGCG 841 AGATCTGTGG CAAGAGCTTC ACCAGCCGGC CCAACATGAA GCGGCACCGG CGCACGCACA 901 CGGGCGAGAA GCCGTACCCG TGCGACGTGT GTGGCCAGCG CTTCCGCTTC TCCAACATGC 961 TCAAGGCCCA CAAGGAGAAG TGCTTCCGCG TCAGCCACAC CCTGGCCGGC GACGGCGTCC 1021 CCGCTGCCCC AGGCCTGCCC CCAACCCAGC CCCAGGCGCA CGCACTGCCC CTGCTCCCGG 1081 GGCTGCCCCA GACCCTGCCG CCCCCGCCCC ACCTGCCGCC CCCGCCTCCG CTCTTCCCCA 1141 CCACTGCCAG CCCCGGCGGG AGGATGAACG CCAACAACTA CCCAACTTTC TTGTACAAAG 1201 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1261 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1321 ACGATCCCGA TCGGGCTCTA ACACCTGAAC GCGTTAAGTC gacaatcaac ctctggatta 1381 caaaatttgt gaaagatt