Construct: ORF TRCN0000479186
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011186.1_s317c1
- Derived from:
- ccsbBroadEn_13414
- DNA Barcode:
- GTAGCTATTCCATAGAGCGTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOK6 (220164)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479186
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220164 | DOK6 | docking protein 6 | XM_017025610.1 | 99.7% | 100% | 30T>C;114A>G |
| 2 | human | 220164 | DOK6 | docking protein 6 | XM_017025611.1 | 99.7% | 100% | 30T>C;114A>G |
| 3 | human | 220164 | DOK6 | docking protein 6 | NM_152721.6 | 67.1% | 67.3% | 1_324del;354T>C;438A>G |
| 4 | human | 220164 | DOK6 | docking protein 6 | XM_011525875.2 | 54.2% | 53.7% | (many diffs) |
| 5 | mouse | 623279 | Dok6 | docking protein 6 | NM_001039173.1 | 61% | 67% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtgtctgggg accaggctca atgacatcag ccttggggag cccgaccttc 121 tggccgcagg agtgcagcgg gaacagaatg agagattcaa cgtgtatctt atgcctacgc 181 caaacctgga tatttatggt gaatgcacaa tgcagatcac tcatgaaaat atctatctct 241 gggatatcca caatgccaag gtcaaactgg tgatgtggcc tctcagctca ctgaggagat 301 acggtcggga cTCAACGTGG TTCACGTTTG AGTCAGGAAG AATGTGTGAC ACAGGAGAAG 361 GACTATTCAC TTTTCAAACA AGGGAAGGAG AAATGATCTA TCAGAAGGTT CATTCTGCGA 421 CACTGGCCAT AGCTGAGCAA CATGAAAGAT TAATGCTAGA AATGGAACAG AAGGCCCGGC 481 TTCAGACAAG CTTGACTGAA CCAATGACAT TATCCAAATC AATATCTCTT CCTCGCAGCG 541 CGTACTGGCA TCACATCACT CGTCAGAACA GCGTTGGTGA AATCTACAGT TTGCAAGGTC 601 ATGGGTTTGG TTCGTCAAAG ATGTCTCGTG CACAGACATT TCCCAGCTAC GCCCCAGAAC 661 AGAGTGAAGA GGCCCAGCAG CCGTTGTCGC GGTCCAGCAG CTATGGATTC AGCTACAGCT 721 CCAGCCTCAT CCAATGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTAGCTATTC CATAGAGCGT 901 CGGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt