Construct: ORF TRCN0000479216
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005293.1_s317c1
- Derived from:
- ccsbBroadEn_12660
- DNA Barcode:
- CAGTGCTACCGAGAATATCCGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MINDY3 (80013)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479216
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XM_011519691.2 | 36.3% | 36.1% | 286A>G;409_1119del |
| 2 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XM_017016671.1 | 34% | 33.9% | 286A>G;409_1194del |
| 3 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XM_006717508.2 | 32.4% | 32.2% | 286A>G;409_1254del |
| 4 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XM_011519690.2 | 31.9% | 31.7% | 286A>G;409_1275del |
| 5 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | NM_024948.4 | 30.4% | 30.3% | 286A>G;409_1335del |
| 6 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XR_001747207.1 | 19% | 1_211del;497A>G;620_2132del | |
| 7 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XR_001747206.1 | 18.4% | 1_211del;497A>G;620_2203del | |
| 8 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XR_930516.2 | 18.3% | 1_211del;497A>G;620_2220del | |
| 9 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XR_001747205.1 | 17.8% | 1_211del;497A>G;620_2284del | |
| 10 | human | 80013 | MINDY3 | MINDY lysine 48 deubiquitin... | XR_428647.2 | 17.6% | 1_211del;497A>G;620_2301del | |
| 11 | mouse | 66960 | Mindy3 | MINDY lysine 48 deubiquitin... | XM_006497523.3 | 29.5% | 29.6% | (many diffs) |
| 12 | mouse | 66960 | Mindy3 | MINDY lysine 48 deubiquitin... | XM_006497522.3 | 29.3% | 29.4% | (many diffs) |
| 13 | mouse | 66960 | Mindy3 | MINDY lysine 48 deubiquitin... | NM_024185.4 | 27.7% | 27.8% | (many diffs) |
| 14 | mouse | 66960 | Mindy3 | MINDY lysine 48 deubiquitin... | XR_001783166.1 | 16.7% | (many diffs) | |
| 15 | mouse | 66960 | Mindy3 | MINDY lysine 48 deubiquitin... | XR_865931.1 | 14.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC CGAACTGACT AAAGAGCTGA TGGAGCTGGT GTGGGGCACC AAGAGCAGCC 121 CCGGTCTCTC GGACACCATT TTCTGCCGCT GGACGCAAGG GTTTGTGTTT AGTGAATCAG 181 AGGGATCTGC ATTAGAACAG TTTGAAGGTG GCCCCTGTGC TGTTATTGCA CCTGTTCAGG 241 CATTTCTTTT GAAGAAGCTC CTGTTTTCTT CGGAGAAGTC TTCTTGGCGG GATTGTTCAG 301 AGGAAGAGCA GAAGGAACTC CTTTGTCATA CCTTGTGTGA TATTTTAGAA GGTGCTTGTT 361 GTGACCACTC TGGATCATAC TGCTTGGTTT CATGGTTAAG AGGAAAGACA ACTGAGGAAA 421 CTGCTAGTAT TTCTGGGAGT CCTGCAGAGT CTAGTTGCCA AGTGGAACAT TCTTACCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAC AGTGCTACCG AGAATATCCG GTAACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att