Transcript: Mouse XM_006497523.3

PREDICTED: Mus musculus family with sequence similarity 188, member A (Fam188a), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mindy3 (66960)
Length:
2283
CDS:
239..1492

Additional Resources:

NCBI RefSeq record:
XM_006497523.3
NBCI Gene record:
Mindy3 (66960)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006497523.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000264565 GAGCGATTTCATGCATTAATT pLKO_005 674 CDS 100% 15.000 12.000 N Mindy3 n/a
2 TRCN0000056111 CAATGGATTGAAGCAGTCAAA pLKO.1 1309 CDS 100% 4.950 3.960 N MINDY3 n/a
3 TRCN0000264567 GACAGACGACACTCCTATTAA pLKO_005 1399 CDS 100% 15.000 10.500 N Mindy3 n/a
4 TRCN0000264566 ATTATAGACCCTGGCTATTTG pLKO_005 1940 3UTR 100% 13.200 9.240 N Mindy3 n/a
5 TRCN0000264568 TAATGGAAGCTTTACGATATT pLKO_005 1011 CDS 100% 13.200 9.240 N Mindy3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006497523.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04165 pDONR223 100% 86.5% 89.6% None (many diffs) n/a
2 ccsbBroad304_04165 pLX_304 0% 86.5% 89.6% V5 (many diffs) n/a
3 ccsbBroadEn_12660 pDONR223 100% 29.5% 29.6% None (many diffs) n/a
4 ccsbBroad304_12660 pLX_304 0% 29.5% 29.6% V5 (many diffs) n/a
5 TRCN0000479216 CAGTGCTACCGAGAATATCCGGTA pLX_317 100% 29.5% 29.6% V5 (many diffs) n/a
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