Construct: ORF TRCN0000479218
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015367.1_s317c1
- Derived from:
- ccsbBroadEn_10455
- DNA Barcode:
- CCCCCAAGGATTTAACAAATGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT11 (23208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479218
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23208 | SYT11 | synaptotagmin 11 | NM_152280.5 | 99.7% | 99.5% | 144G>C;546T>C;1291T>A |
| 2 | human | 23208 | SYT11 | synaptotagmin 11 | XM_005245014.3 | 99.5% | 99% | (many diffs) |
| 3 | human | 23208 | SYT11 | synaptotagmin 11 | XM_017000759.2 | 99% | 98.6% | (many diffs) |
| 4 | mouse | 229521 | Syt11 | synaptotagmin XI | NM_018804.3 | 89.8% | 95.1% | (many diffs) |
| 5 | mouse | 229521 | Syt11 | synaptotagmin XI | XM_006501348.1 | 89.8% | 95.1% | (many diffs) |
| 6 | mouse | 229521 | Syt11 | synaptotagmin XI | XM_006501350.2 | 89.6% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaataag 61 ttggcatggc tgagatcacc aatatccgac ctagctttga tgtgtcaccg gtggtggccg 121 gcctcatcgg ggcctctgtg ctggtggtgt gtgtctcggt gaccgtcttt gtctggtcat 181 gctgccacca gcaggcagag aagaagcaca agaacccacc atacaagttt attcacatgc 241 tcaaaggcat cagcatatac ccagagaccc tcagcaacaa gaagaaaatc atcaaagtgc 301 ggagagacaa agatggtcct gggagggaag gtggacgtag gaacctgttg gtggacgcag 361 cagaggctgg cctgctaagc cgagacaaag atcccagggg gcctagctct ggatcttgta 421 tagaccaatt acccatcaaa atggactatg gggaagaact aaggagccct attacaagcc 481 tgacccctgg ggagagcaaa accacctctc catcatctcc agaggaggat gtcatgctag 541 gatccctcac cttctcagtg gactataact tcccgaaaaa agccctggtg gtgacaatcc 601 aggaggccca cgggctgcca gtgatggatg accagaccca gggatctgac ccctacatca 661 aaatgaccat ccttcctgac aaacggcatc gggtgaagac cagagtgctg cggaagaccc 721 tggaccctgt gtttgacgag accttcacct tctatggcat cccctacagc cagctgcagg 781 acctggtgct gcacttcctt gtcctcagct ttgaccgctt ctctcgggat gatgtcattg 841 gcgaggtcat ggtgccactg gcaggggtgg accccagcac aggcaaggta caactgacca 901 gggacatcat caaaaggaat aTCCAGAAGT GCATCAGCAG AGGGGAGCTC CAGGTGTCTC 961 TGTCATATCA GCCTGTGGCA CAGAGAATGA CAGTGGTGGT CCTCAAAGCC AGACACTTGC 1021 CGAAGATGGA TATCACCGGT CTCTCAGGTA ATCCTTATGT CAAGGTGAAC GTCTACTACG 1081 GCAGAAAGCG CATTGCCAAG AAGAAAACCC ATGTGAAGAA GTGCACTTTG AACCCCATCT 1141 TCAATGAATC TTTCATCTAC GACATCCCCA CTGACCTCCT GCCTGATATC AGCATCGAGT 1201 TCCTCGTTAT CGACTTCGAT CGCACCACCA AGAATGAGGT GGTGGGGAGG CTGATCCTGG 1261 GGGCACACAG TGTCACAGCC AGTGGTGCTG AACACTGGAG AGAGGTCTGC GAGAGCCCCC 1321 GCAAGCCTGT GGCCAAGTGG CACAGTCTGA GCGAGAACTA CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGACCCCCA AGGATTTAAC AAATGCCTAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt