Construct: ORF TRCN0000479225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016581.1_s317c1
- Derived from:
- ccsbBroadEn_11570
- DNA Barcode:
- GTACAGGTACTCAAGTAGGGTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAPOLA (10914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_001252006.1 | 100% | 100% | |
| 2 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_001252007.1 | 78% | 73.8% | (many diffs) |
| 3 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_001363666.2 | 40.6% | 39.7% | (many diffs) |
| 4 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_001363665.2 | 40.6% | 39.6% | (many diffs) |
| 5 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_001363664.2 | 39.2% | 38.3% | (many diffs) |
| 6 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_001363662.2 | 39.2% | 38.3% | (many diffs) |
| 7 | human | 56903 | PAPOLB | poly(A) polymerase beta | NM_020144.5 | 38.6% | 40% | (many diffs) |
| 8 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_001293627.1 | 38.1% | 37.2% | (many diffs) |
| 9 | human | 10914 | PAPOLA | poly(A) polymerase alpha | NM_032632.4 | 38.1% | 37.2% | (many diffs) |
| 10 | mouse | 18789 | Papola | poly (A) polymerase alpha | XR_381486.2 | 62.1% | (many diffs) | |
| 11 | mouse | 56522 | Papolb | poly (A) polymerase beta (t... | NM_019943.2 | 38.3% | 39.9% | (many diffs) |
| 12 | mouse | 18789 | Papola | poly (A) polymerase alpha | XM_006515595.2 | 37.4% | 38.7% | (many diffs) |
| 13 | mouse | 18789 | Papola | poly (A) polymerase alpha | XM_017314994.1 | 36.9% | 38.4% | (many diffs) |
| 14 | mouse | 18789 | Papola | poly (A) polymerase alpha | NM_001347440.1 | 36.8% | 38.3% | (many diffs) |
| 15 | mouse | 18789 | Papola | poly (A) polymerase alpha | XM_006515594.2 | 36.3% | 37.5% | (many diffs) |
| 16 | mouse | 18789 | Papola | poly (A) polymerase alpha | XM_006515593.3 | 36% | 37.2% | (many diffs) |
| 17 | mouse | 18789 | Papola | poly (A) polymerase alpha | XM_017314992.1 | 35.8% | 37.3% | (many diffs) |
| 18 | mouse | 18789 | Papola | poly (A) polymerase alpha | NM_011112.4 | 35.8% | 37.2% | (many diffs) |
| 19 | mouse | 18789 | Papola | poly (A) polymerase alpha | XM_006515592.2 | 35% | 36.2% | (many diffs) |
| 20 | mouse | 18789 | Papola | poly (A) polymerase alpha | XM_006515596.3 | 35% | 36.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 921
- ORF length:
- 855
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtttccagtt acaacacagg gatcacaaca aacacaaccg ccacagaagc 121 actatggcat tacttctcct atcagcttag cagcccccaa ggagactgac tgcgtactta 181 cacagaaact aattgagaca ttgaaaccct ttggggtttt tgaagaggaa gaggaactgc 241 agcgcaggat tttaattttg ggaaaactaa ataacctggt aaaagagtgg atacgagaaa 301 tcagtgaaag caagaatctt ccacaatctg taattgaaaa tgttggagga aaaattttta 361 catttggatc TTACAGATTA GGAGTGCATA CAAAAGGTGC TGATATTGAT GCGTTGTGTG 421 TTGCACCAAG ACATGTTGAT CGAAGTGACT TTTTCACCTC ATTCTATGAT AAGTTGAAAT 481 TACAGGAAGA AGTAAAAGAT TTAAGAGCTG TTGAAGAGGC ATTCGTACCA GTTATTAAAC 541 TCTGTTTTGA TGGGATAGAG ATTGATATTT TGTTTGCAAG ATTAGCACTG CAGACAATTC 601 CTGAAGATTT GGATCTACGA GATGACAGTC TGCTAAAAAA TTTAGATATA AGATGTATAA 661 GAAGTCTTAA CGGTTGCAGG GTAACCGATG AAATTTTACA TCTAGTACCA AACATTGACA 721 ACTTCAGGTT AACTCTGAGA GCTATCAAAC TATGGGCCAA ACGCCACAAC ATCTATTCCA 781 ATATATTAGG TTTCCTCGGT GGTGTTTCCT GGGCTATGCT AGTAGCAAGA ACTTGCCAGC 841 TTTATCCAAA TGCAATAGCA TCAACTCTTG TACATAAATT TTTCTTGGTA TTTTCTAAAT 901 GGTATGTGTT TAGATTATAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTAC AGGTACTCAA 1081 GTAGGGTTCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt