Transcript: Mouse NM_019943.2

Mus musculus poly (A) polymerase beta (testis specific) (Papolb), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Papolb (56522)
Length:
2337
CDS:
191..2119

Additional Resources:

NCBI RefSeq record:
NM_019943.2
NBCI Gene record:
Papolb (56522)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019943.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000250053 ACGAACATACCCAATCCTATA pLKO_005 2087 CDS 100% 10.800 15.120 N Papolb n/a
2 TRCN0000250054 ACGTTCGGCTCTTACCGATTA pLKO_005 488 CDS 100% 10.800 15.120 N Papolb n/a
3 TRCN0000257984 CAGAAGGGAAGAACCAGATAG pLKO_005 2163 3UTR 100% 10.800 7.560 N Papolb n/a
4 TRCN0000250051 CGACCAGGATGGTCATGATTG pLKO_005 1188 CDS 100% 10.800 7.560 N Papolb n/a
5 TRCN0000250052 GATTGCTGCAATGCATCTAAG pLKO_005 1639 CDS 100% 10.800 7.560 N Papolb n/a
6 TRCN0000053020 GCAGCATCTGTGACCAACATA pLKO.1 1880 CDS 100% 5.625 3.375 N PAPOLA n/a
7 TRCN0000298258 GCAGCATCTGTGACCAACATA pLKO_005 1880 CDS 100% 5.625 3.375 N PAPOLA n/a
8 TRCN0000294191 ACCATCTTATGCCTATAATTA pLKO_005 1122 CDS 100% 15.000 7.500 Y PAPOLA n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019943.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08657 pDONR223 100% 84.6% 84.5% None (many diffs) n/a
2 ccsbBroad304_08657 pLX_304 0% 84.6% 84.5% V5 (many diffs) n/a
3 TRCN0000468084 CTTTTCATCAAGCTAGGAGATCAC pLX_317 24.9% 84.6% 84.5% V5 (many diffs) n/a
4 ccsbBroadEn_11570 pDONR223 100% 38.3% 39.9% None (many diffs) n/a
5 TRCN0000479225 GTACAGGTACTCAAGTAGGGTTCA pLX_317 64.1% 38.3% 39.9% V5 (many diffs) n/a
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