Construct: ORF TRCN0000479228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004132.1_s317c1
- Derived from:
- ccsbBroadEn_02471
- DNA Barcode:
- GGAGTCTAGCTTAAGCTTTCGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CXCL13 (10563)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479228
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10563 | CXCL13 | C-X-C motif chemokine ligan... | NM_001371558.1 | 99.6% | 100% | 318A>G |
| 2 | human | 10563 | CXCL13 | C-X-C motif chemokine ligan... | NM_006419.2 | 99.6% | 100% | 318A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 417
- ORF length:
- 327
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAGTTCAT CTCGACATCT CTGCTTCTCA 121 TGCTGCTGGT CAGCAGCCTC TCTCCAGTCC AAGGTGTTCT GGAGGTCTAT TACACAAGCT 181 TGAGGTGTAG ATGTGTCCAA GAGAGCTCAG TCTTTATCCC TAGACGCTTC ATTGATCGAA 241 TTCAAATCTT GCCCCGTGGG AATGGTTGTC CAAGAAAAGA AATCATAGTC TGGAAGAAGA 301 ACAAGTCAAT TGTGTGTGTG GACCCTCAAG CTGAATGGAT ACAAAGAATG ATGGAAGTAT 361 TGAGAAAAAG AAGTTCTTCA ACTCTACCAG TTCCAGTGTT TAAGAGGAAG ATTCCCTGCC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GAGGAGTCTA GCTTAAGCTT TCGAAAACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt