Construct: ORF TRCN0000479244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001190.1_s317c1
- Derived from:
- ccsbBroadEn_11716
- DNA Barcode:
- CACACGAATACCCCGGTTTGGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSL6 (23305)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479244
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23305 | ACSL6 | acyl-CoA synthetase long ch... | NM_001205251.1 | 99.8% | 99.8% | 42G>A;665A>C;747A>G |
| 2 | human | 23305 | ACSL6 | acyl-CoA synthetase long ch... | NM_001205247.2 | 90.3% | 90.3% | (many diffs) |
| 3 | human | 23305 | ACSL6 | acyl-CoA synthetase long ch... | NM_001205248.2 | 89% | 89% | (many diffs) |
| 4 | human | 23305 | ACSL6 | acyl-CoA synthetase long ch... | NM_001205250.1 | 87.7% | 87.7% | (many diffs) |
| 5 | human | 23305 | ACSL6 | acyl-CoA synthetase long ch... | NM_001009185.3 | 86% | 86% | (many diffs) |
| 6 | human | 23305 | ACSL6 | acyl-CoA synthetase long ch... | NM_015256.4 | 86% | 86% | (many diffs) |
| 7 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | NM_001033598.1 | 78.7% | 81.4% | (many diffs) |
| 8 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | NM_001033599.1 | 78.7% | 81.4% | (many diffs) |
| 9 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | XM_006532843.1 | 78.7% | 81.4% | (many diffs) |
| 10 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | XM_006532844.1 | 78.7% | 81.4% | (many diffs) |
| 11 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | XM_006532845.2 | 78.7% | 81.4% | (many diffs) |
| 12 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | XM_017314444.1 | 78.7% | 81.4% | (many diffs) |
| 13 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | NM_001033597.1 | 76% | 78.6% | (many diffs) |
| 14 | mouse | 216739 | Acsl6 | acyl-CoA synthetase long-ch... | NM_144823.4 | 76% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1932
- ORF length:
- 1866
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gacacaggag atcctgagga tactgcgact gcctgaacta ggtgacttgg 121 gacagttttt ccgcagcctc tcggccacca ccctcgacag tggcggggca cggcgatctg 181 tgattgggtc tggccctcag ctacttaccc actactatga tgatgcccgg accatgtacc 241 aggtgttccg ccgtgggctt agcatctcag ggaatgggcc ctgtcttggt ttcaggaagc 301 ctaagcagcc ttaccagtgg ctgtcctacc aggaggtggc cgacagggct gaatttctgg 361 ggtccggact tctccagcac aattgtaaag catgcactga tcagtttatt ggtgtttttg 421 cacaaaatcg gccagagtgg atcattgtgg agctggcctg ctacacatat tccatggtgg 481 tggtcccgct ctatgacacc ctgggccctg gggctatccg ctacatcatc aatacagcgg 541 acatcagcac cgtgattgtg gacaaacctc agaaggctgt gcttctgcta gagcatgtgg 601 agaggaagga gactccaggc ctcaagctga tcatcctcat ggacccattc gaagaagccc 661 tgaaagagag agggcagaag tgcggggtgg tcattaagtc catgcaggcc gtggaggact 721 gtggccaagc gaatcaccag gctcctgtgc ccccgcagcc tgatgacctc tccattgtgt 781 gtttcacaag cggcacgaca gggaacccaa agggtgcgat gctcacccat gggaacgtgg 841 tggctgattt ctcaggcttt ctgaaagtga cagagggaga tatccgcctt ctctcagatg 901 acatgaaggc tctatgcccc accatcttcc ctgtggtccc acgactgctg aaccggatgt 961 acgacaagat cttcagccag gcaaacacac cattaaagcg ctggctcctg gagtttgcag 1021 caaagcgtaa gcaagccgag gtccggagtg gaatcatcag gaatgatagt atctgggatg 1081 aactcttctt taataagatt caggccagtc ttggtgggtg tgtgcggatg attgttactg 1141 gagcagcccc agcatcacca acagttctgg gatttctccg ggcagctcta gggtgccagg 1201 tttatgaagg ttatggccaa actgagtgca cagctggatg taccttcacc actcctggcg 1261 actggacctc agggcacgta ggggcgccac ttccctgcaa tcatatcaag ctcgttgatg 1321 ttgaggaact gaactactgg gcctgcaaag gagagggaga gatatgtgtg agaggaccaa 1381 atgtgttcaa aggctacttg aaagatccag acaggacgaa ggaggccctg gacagcgatg 1441 gctggcttca cactggagac atcggaaaat ggctgccggc aggaactctt aaaattattg 1501 atcggaaaaa gcatatattt aaacttgctc agggagaata tgttgcaccc gagaagattg 1561 agaacatcta catccggagc caacctgtgg cgcaaatcta tgtccatggg gacagcttaa 1621 aggccttttt ggtaggcatt gttgtgcctg accctgaagt tatgccctcc tgggcccaga 1681 agagaggaat tgaaggaaca tatgcagatc tctgcacaaa taaggatctg aagaaagcca 1741 ttttggaaga tatggtgagg ttaggaaaag aaagtggact ccattctttt gagcaggtta 1801 aagccattca catccattcT GACATGTTCT CAGTTCAAAA TGGCTTGCTG ACACCAACAC 1861 TAAAAGCTAA GAGACCTGAG CTGAGAGAGT ACTTCAAAAA ACAAATAGAA GAGCTTTACT 1921 CAATCTCCAT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1981 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2041 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAC ACGAATACCC CGGTTTGGTA 2101 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t