Construct: ORF TRCN0000479275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007578.1_s317c1
- Derived from:
- ccsbBroadEn_09246
- DNA Barcode:
- TCCGCGGCCGAGTAGCCTACCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CGNL1 (84952)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84952 | CGNL1 | cingulin like 1 | NM_001252335.2 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 84952 | CGNL1 | cingulin like 1 | NM_032866.5 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 84952 | CGNL1 | cingulin like 1 | XM_017022686.1 | 99.8% | 99.7% | (many diffs) |
| 4 | human | 84952 | CGNL1 | cingulin like 1 | XM_005254726.4 | 99.8% | 99.6% | (many diffs) |
| 5 | human | 84952 | CGNL1 | cingulin like 1 | XM_005254727.5 | 99.8% | 99.6% | (many diffs) |
| 6 | human | 84952 | CGNL1 | cingulin like 1 | XM_011522120.1 | 99.8% | 99.6% | (many diffs) |
| 7 | human | 84952 | CGNL1 | cingulin like 1 | XM_011522121.2 | 99.8% | 99.6% | (many diffs) |
| 8 | human | 84952 | CGNL1 | cingulin like 1 | XM_017022685.1 | 99.8% | 99.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3975
- ORF length:
- 3906
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctgtat ttcggtgaat atcaacatgt gcagcaggaa tatggggtcc 121 atctgagact cgcaagtgat gatacccaaa aatcaaggag ttcccagaac tccaaggcag 181 gctcctacgg tgtcagtatt cgggtccagg gaattgatgg tcacccctat attgtcctga 241 ataacacaga acggtgccta gcaggcacat cgttttctga aaatgggcca ccctttccac 301 ctccagtgat aaataacctg cctctacatt ccagcaatgg ttctgtgcca aaggagaaca 361 gtgaagaact tcagcttcca gaaaacccat acgcccagcc tagcccaata agaaacctga 421 aacagcccct gctccatgag ggcaagaatg gagttctaga tcgcaaagac gggtctgtga 481 agccatctca cctgctgaac tttcagaggc atccagagct tttgcaaccc tatgaccctg 541 aaaagaatga gttgaattta caaaatcacc agccttctga gagtaattgg ctaaaaacgt 601 tgacagaaga aggcatcaac aataagaagc cttggacttg ctttcccaaa cctagcaatt 661 cccagcctac cagtccctcc ttggaagacc cggccaaatc tggtgtgaca gctattcgtt 721 tatgcagctc cgtggtcata gaggacccca aaaagcagac ctcagtgtgt gtaaacgttc 781 agagctgcac caaggagagg gtgggagagg aggccctttt cactagcggg aggcccctga 841 ctgcccacag cccacatgcc caccctgaaa ccaagaaaac caggccagat gttcttccct 901 tccggcgaca ggattcagcg ggacccgtcc tggatggagc tcggtcccgg aggtcctcct 961 cgtcatccac aactcccacg tcagccaact ctttgtacag gtttttactg gatgatcagg 1021 aatgtgccat ccatgccgac aacgtcaatc gtcatgaaaa cagaaggtat attcccttcc 1081 tgccaggaac tggacgggat attgatacag gatcaattcc tggtgtggat cagttaattg 1141 aaaaatttga tcaaaaacct gggcttcaga gaagaggaag gtctgggaag cgaaacagaa 1201 ttaatccaga tgacaggaaa agatccagaa gcgtggatag cgcctttcct tttggcctcc 1261 aagggaactc ggagtacctg attgaattca gtaggaactt gggcaagtca agcgaacacc 1321 tcctccggcc ttcccaggtg tgcccgcagc ggccactgtc tcaggagcgc cgtgggaaac 1381 agagcgtggg ccgcaccttt gcaaagctgc agggagcagc gcacggggct tcatgtgccc 1441 acttcaggcc tccccagccg aacatagatg ggaaagttct ggaaaccgaa ggtagtcagg 1501 aaagtacagt gatccgtgcg ccctcccttg gtgcacagag taaaaaggag gaggaggtga 1561 aaacagccac cgctacgctg atgttacaga accgggcagc agcaacttcg cctgattctg 1621 gtgccaagaa aatttccgtg aagacatttc cttcggcctc aaatactcag gccacaccgg 1681 atctcttaaa gggccagcaa gagctcactc agcaaaccaa tgaggagaca gctaagcaga 1741 ttctctacaa ttacctcaaa gaaggaagca ctgataatga cgatgctact aaaaggaaag 1801 tcaacttggt ctttgagaaa atccagacct taaagtctcg agcagctggg agcgcccaag 1861 gaaataacca agcttgtaat tccacatctg aagtcaaaga tctattggaa cagaaaagca 1921 agttgaccat agaagtggct gaacttcaga gacagcttca actggaagtc aagaatcaac 1981 agaacattaa agaagagaga gagaggatga gagcaaacct agaagagctc cgaagccaac 2041 acaacgaaaa ggtggaggag aactccacat tgcagcaacg actggaagaa agtgaagggg 2101 agctccggaa gaatctggag gagctattcc aggtgaagat ggaacgggag cagcatcaga 2161 ctgagatcag ggatctccag gaccagctct cagaaatgca cgatgaactg gacagtgcaa 2221 agcgatcgga ggacagggag aagggagctc tgattgagga gctcttacag gcaaaacagg 2281 atcttcaaga tctgctgatt gccaaagagg agcaagaaga cctcttgaga aagcgagagc 2341 gtgaactcac cgccctgaag ggagccctga aagaagaggt ttccagccat gatcaggaga 2401 tggacaagct gaaggagcaa tatgatgctg agttgcaggc cctgagggag agtgtggaag 2461 aagcaaccaa gaatgtcgag gtcttggcga gcaggagcaa cacttcagag caagaccagg 2521 cggggactga aatgcgcgtg aagcttctgc aggaggagaa tgagaagctg cagggaagaa 2581 gcgaagagct ggagcggaga gttgctcagc ttcaaaggca gatcgaggac ctgaaaggcg 2641 atgaagccaa ggcgaaggaa acgctgaaga agtacgaggg agaaatacga cagttagagg 2701 aggcccttgt gcacgccaga aaggaagaaa aagaagctgt gtcagccaga agggccctgg 2761 agaatgaact ggaggctgct cagggaaatc tgagtcagac tacccaggag cagaagcagt 2821 tgtctgagaa gctcaaagag gagagtgagc agaaggagca gctaagaagg ttgaagaacg 2881 agatggagaa tgagcggtgg cacctgggca aaaccattga gaaactgcag aaggagatgg 2941 cagacattgt tgaggcctcc cgtacctcaa ccctggagct ccagaaccag ctggatgagt 3001 ataaggagaa aaaccgcagg gagctcgcag aaatgcaaag acagttgaag gagaaaacgc 3061 tggaggcaga aaagtcccga ctgacagcca tgaaaatgca ggatgagatg cgtctgatgg 3121 aggaagagtt acgggactac cagagagctc aggatgaagc actcacaaaa aggcagcttc 3181 tggagcagac gctgaaggac ctggagtatg agctggaagc caagagtcac ctcaaagatg 3241 accgcagcag gctggtcaag cagatggagg acaaggtgtc tcaactggag atggaactgg 3301 aagaagagag aaacaactca gatttgctgt ctgagaggat cagtaggagc agggaacaga 3361 tggagcagtt gaggaatgag ctacttcagg agagagctgc gagacaagac ttggagtgcg 3421 ataagatttc cctggagagg cagaacaagg acttaaagag ccggattatc cacctggaag 3481 gttcctacag gtccagcaaa gaggggctgg ttgtgcagat ggaggccagg atcgcggagc 3541 tggaggaccg cctggagagt gaggagaggg atcgggccaa tcttcagcTC AGCAACCGGC 3601 GGCTGGAGCG GAAAGTGAAG GAGCTGGTGA TGCAGGTGGA TGATGAGCAC CTGTCATTGA 3661 CTGATCAGAA GGACCAGCTG AGCTTGCGTT TGAAAGCCAT GAAGCGGCAG GTGGAGGAGG 3721 CTGAGGAGGA AATCGACAGA CTGGAAAGTT CTAAAAAGAA GCTGCAGAGG GAGCTGGAGG 3781 AGCAGATGGA CATGAATGAG CATCTGCAGG GGCAGCTCAA CTCCATGAAG AAGGACTTAA 3841 GACTGAAGAA GCTGCCGAGT AAAGTGCTGG ATGACATGGA TGACGACGAT GACCTCAGCA 3901 CGGATGGGGG AAGCCTCTAT GAGGCGCCTG TGAGCTACAC ATTCTCCAAG GACAGCACCG 3961 TCGCCAGCCA GATCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 4021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 4081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCCGCGGCCG AGTAGCCTAC 4141 CATAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt