Transcript: Human NM_032866.5

Homo sapiens cingulin like 1 (CGNL1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
CGNL1 (84952)
Length:
7214
CDS:
79..3987

Additional Resources:

NCBI RefSeq record:
NM_032866.5
NBCI Gene record:
CGNL1 (84952)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032866.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000139679 CGCTACGCTGATGTTACAGAA pLKO.1 1581 CDS 100% 4.950 6.930 N CGNL1 n/a
2 TRCN0000217916 GACTTAAAGAGCCGGATTATC pLKO.1 3460 CDS 100% 13.200 10.560 N Cgnl1 n/a
3 TRCN0000428067 GGACTTAAAGAGCCGGATTAT pLKO_005 3459 CDS 100% 13.200 10.560 N CGNL1 n/a
4 TRCN0000139626 CATCTGAGACTCGCAAGTGAT pLKO.1 130 CDS 100% 4.950 3.960 N CGNL1 n/a
5 TRCN0000140917 CTGAAGAAGCTGCCGAGTAAA pLKO.1 3853 CDS 100% 13.200 9.240 N CGNL1 n/a
6 TRCN0000140784 GCAGACCTCAGTGTGTGTAAA pLKO.1 765 CDS 100% 13.200 9.240 N CGNL1 n/a
7 TRCN0000437556 TATGCAGCTCCGTGGTCATAG pLKO_005 731 CDS 100% 10.800 7.560 N CGNL1 n/a
8 TRCN0000140338 CCATCTCACCTGCTGAACTTT pLKO.1 493 CDS 100% 5.625 3.938 N CGNL1 n/a
9 TRCN0000139202 CAGCCGAACATAGATGGGAAA pLKO.1 1465 CDS 100% 4.050 2.835 N CGNL1 n/a
10 TRCN0000141906 GCACTGATAATGACGATGCTA pLKO.1 1778 CDS 100% 3.000 2.100 N CGNL1 n/a
11 TRCN0000139825 CAAGGACTTAAAGAGCCGGAT pLKO.1 3456 CDS 100% 2.160 1.512 N CGNL1 n/a
12 TRCN0000182290 GAGGAGAATGAGAAGCTGCTA pLKO.1 2563 CDS 100% 2.640 1.584 N Ccdc155 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032866.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09246 pDONR223 100% 99.8% 99.7% None (many diffs) n/a
2 ccsbBroad304_09246 pLX_304 0% 99.8% 99.7% V5 (many diffs) n/a
3 TRCN0000479275 TCCGCGGCCGAGTAGCCTACCATA pLX_317 10.1% 99.8% 99.7% V5 (many diffs) n/a
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