Construct: ORF TRCN0000479277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004091.1_s317c1
- Derived from:
- ccsbBroadEn_11303
- DNA Barcode:
- TTGTGGGATGGCGTGAGGCGCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS9 (8787)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8787 | RGS9 | regulator of G protein sign... | NM_001081955.3 | 92.5% | 92.5% | 1_147del;357C>G;826C>A |
| 2 | human | 8787 | RGS9 | regulator of G protein sign... | NM_003835.4 | 92.1% | 92.1% | (many diffs) |
| 3 | human | 8787 | RGS9 | regulator of G protein sign... | NM_001165933.2 | 63.4% | 62.4% | (many diffs) |
| 4 | mouse | 19739 | Rgs9 | regulator of G-protein sign... | NM_011268.2 | 78.9% | 83.3% | (many diffs) |
| 5 | mouse | 19739 | Rgs9 | regulator of G-protein sign... | XM_011248828.2 | 76.2% | 78.1% | (many diffs) |
| 6 | mouse | 19739 | Rgs9 | regulator of G-protein sign... | NM_001165934.1 | 56.5% | 59.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1935
- ORF length:
- 1866
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaggaagt gatgttctgc aatggatcgt ccagcggctt tggatctcca 121 gtctggaggc acagaacttg ggcaacttta ttgtcaggta tggctacatt taccccctgc 181 aagaccccaa gaatctcatt ctcaagcctg atggcagcct ctacagattt cagacaccgt 241 atttctggcc cacccagcag tggccagctg aagatacgga ttacgccatc tatctggcca 301 agcgaaatat caaaaagaaa gggattttgg aagaatatga aaaggaaaat tacaatttct 361 tgaaccaaaa aatgaactat aagtgggact ttgtcattat gcaggccaaa gagcagtaca 421 gggctggaaa ggagaggaac aaagcagaca gatatgccct ggactgccag gagaaggcat 481 actggctggt gcaccgatgc cctcctggaa tggacaatgt gctggactac ggcctggacc 541 gagtgaccaa tccgaatgaa gtcaagaaac aaacagtcgt tgctgtcaaa aaagagatca 601 tgtattacca acaggccttg atgaggtcca cagtgaagtc ttctgtgtcc ctgggaggga 661 ttgtgaaata cagtgagcag ttctcatcca acgatgccat catgtcaggc tgcctcccca 721 gcaacccctg gatcaccgat gacaccaagt tctgggactt aaatgccaaa ttggtggaaa 781 tcccaaccaa gatgcgagtg gaacgatggg ccttcaactt cagcgaattg atccgagacc 841 ccaaaggtcg acagagcttc cagtacttcc tcaagaaaga attcagtgga gagaatctgg 901 gattctggga agcctgcgag gatctgaagt atggagatca gtccaaagtc aaggagaaag 961 cagaggagat ttacaagctg ttcctggccc cgggggcgag gcgctggatc aacatagatg 1021 gcaaaaccat ggacatcaca gtgaaggggc tgaagcaccc ccaccgctat gtgctggacg 1081 ccgcacaaac ccacatttac atgctcatga agaaggattc ttatgctcgc tatttaaaat 1141 ctccgatcta taaggacatg ctggccaaag ctattgaacc tcaggaaacc accaagaaaa 1201 gctccaccct cccttttatg cggcgtcacc tgcgctccag cccaagccct gtcatcctga 1261 gacagctgga agaggaagcc aaggcccgag aagcagccaa cactgtggac atcacccagc 1321 cgggccagca catggctccc agcccccatc tgaccgtgta caccgggacc tgcatgcccc 1381 cgtctccttc tagccccttc tcctcctcct gccgctcccc caggaagcct ttcgcctcac 1441 ccagccgctt catccggcga cccagcacca ccatctgccc ctcacccatc agagtggcct 1501 tggagagctc atcgggcttg gagcagaaag gggagtgcag cgggtccatg gccccccgtg 1561 ggccctctgt caccgagagc agcgaggcct ccctcgacac ctcctggcct cgcagccggc 1621 ccagggcccc tcctaaggcc cgcatggctc tgtccTTCAG CAGGTTTCTG AGACGAGGCT 1681 GTCTGGCCTC ACCTGTCTTT GCCAGGCTCT CACCCAAGTG CCCTGCTGTG TCCCACGGGA 1741 GGGTGCAGCC CCTGGGGGAC GTGGGCCAGC AGCTGCCACG ATTGAAATCC AAGAGAGTAG 1801 CAAACTTTTT CCAGATCAAA ATGGATGTGC CCACGGGGAG CGGGACCTGC TTGATGGACT 1861 CGGAGGATGC TGGAACAGGA GAGTCGGGTG ACCGGGCCAC AGAAAAGGAG GTCATCTGCC 1921 CCTGGGAGAG CCTGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1981 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2041 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTGTGGGATG GCGTGAGGCG 2101 CAGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt