Construct: ORF TRCN0000479280
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018540.1_s317c1
- Derived from:
- ccsbBroadEn_11545
- DNA Barcode:
- AAACCTGTAGTTTTCCCTACCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN9 (10753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479280
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10753 | CAPN9 | calpain 9 | NM_001319676.1 | 99.9% | 99.8% | 775A>C |
| 2 | human | 10753 | CAPN9 | calpain 9 | NM_006615.3 | 90.8% | 90.7% | 213_401del;964A>C |
| 3 | human | 10753 | CAPN9 | calpain 9 | XM_011544019.2 | 89.5% | 89.7% | 775A>C;1798_1799ins59;1823_1974del |
| 4 | human | 10753 | CAPN9 | calpain 9 | NM_016452.2 | 87% | 86.8% | 213_401del;874_875ins78;886A>C |
| 5 | human | 10753 | CAPN9 | calpain 9 | XM_011544017.1 | 81.9% | 82% | (many diffs) |
| 6 | human | 10753 | CAPN9 | calpain 9 | XM_017000099.1 | 78.8% | 78.7% | 0_1ins396;379A>C |
| 7 | human | 10753 | CAPN9 | calpain 9 | XM_011544018.1 | 78.4% | 78.3% | (many diffs) |
| 8 | human | 10753 | CAPN9 | calpain 9 | XM_017000098.1 | 78.1% | 78.2% | (many diffs) |
| 9 | human | 10753 | CAPN9 | calpain 9 | XR_001736933.1 | 73.5% | (many diffs) | |
| 10 | human | 10753 | CAPN9 | calpain 9 | XM_011544020.1 | 70% | 70% | (many diffs) |
| 11 | human | 10753 | CAPN9 | calpain 9 | XR_949127.1 | 61.7% | (many diffs) | |
| 12 | human | 10753 | CAPN9 | calpain 9 | XM_024452513.1 | 51.9% | 51.7% | (many diffs) |
| 13 | human | 10753 | CAPN9 | calpain 9 | XM_024452514.1 | 51.9% | 51.7% | (many diffs) |
| 14 | mouse | 73647 | Capn9 | calpain 9 | NM_023709.4 | 75.5% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1947
- ORF length:
- 1881
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ttacctctac cgggccccag ggcctcaggc acacccggtt cccaaggacg 121 cccggatcac ccactcctca ggccagagct ttgagcaaat gaggcaggag tgcctgcaga 181 gaggcaccct gtttgaggat gcagacttcc cagccagcaa ttcctccctg ttctacagtg 241 agaggccgca gatccccttt gtgtggaaac gaccagggtt ctggcagcac agtgagtggc 301 tggacgtggt gatcgatgac cgcctgccca ccttcaggga ccgcttggtt ttcctccact 361 ctgccgacca caacgagttc tggagcgcct tgctggaaaa agcctacgcc aagctaaatg 421 ggagctatga agctctgaag ggaggcagcg ccatcgaggc catggaagac ttcactgggg 481 gtgtggcaga gaccttccaa actaaagagg cccccgagaa cttctatgag attctagaga 541 aggctttgaa gagaggctcc ctgctgggct gcttcattga taccagaagt gctgcagaat 601 ctgaggcccg gacgccgttt ggtcttatta agggtcatgc ctacagtgta acgggaattg 661 accaggtaag cttccgaggc cagagaatcg agctcatccg aatccggaac ccttggggcc 721 aggttgagtg gaacgggtcg tggagcgaca gttctccgga gtggcgttct gttggtccag 781 ctgagcagaa gcgtctgtgt cacactgctc tggatgatgg ggaattctgg atggcatttc 841 aggacttcaa ggcccacttt gataaagtgg agatctgcaa cctcactccc gatgccctgg 901 aggaagacgc gatccacaaa tgggaggtga cggtccatca gggaagctgg gttcgcggct 961 ccacggctgg gggctgccgc aatttcctgg ataccttttg gaccaatcca caaataaaat 1021 tgtctctgac tgagaaagat gaggggcagg aggagtgtag tttccttgta gccctgatgc 1081 agaaagatag aaggaaactc aagagatttg gtgccaatgt gctgacaatc ggctatgcca 1141 tttatgagtg ccctgacaaa gacgaacacc tgaacaaaga cttcttcaga taccacgctt 1201 ctcgggccag aagcaagacg ttcatcaacc tgagagaagt ctccgaccgg ttcaagctgc 1261 cccctgggga gtacatcctg attcccagca cttttgagcc ccaccaggaa gctgatttct 1321 gtctgagaat cttttcagag aaaaaagcca ttacccggga tatggatgga aatgtagaca 1381 ttgaccttcc tgagcctcca aagccaactc cacctgacca ggagacagag gaggagcagc 1441 ggtttcgggc tctgtttgaa caagtcgctg gtgaggacat ggaggtgaca gcagaggaac 1501 ttgagtatgt tttaaatgct gtgctgcaaa agaaaaagga catcaaattc aagaagctaa 1561 gcctgatctc ctgtaaaaac atcatttccc TGATGGACAC CAGCGGCAAT GGGAAGCTGG 1621 AGTTTGATGA ATTCAAAGTG TTCTGGGACA AGCTGAAGCA GTGGATTAAC CTTTTCCTTC 1681 GGTTTGATGC TGACAAGTCC GGCACCATGT CTACCTATGA ACTACGGACT GCACTGAAAG 1741 CTGCAGGCTT TCAGCTGAGC AGCCACCTCC TGCAGCTGAT TGTGCTCAGG TATGCGGATG 1801 AGGAGCTCCA GCTGGACTTC GATGACTTCC TCAACTGCCT GGTCCGGCTG GAGAATGCGA 1861 GCCGGGTGTT CCAGGCTCTC AGTACAAAGA ACAAGGAGTT CATTCATCTC AATATAAATG 1921 AGTTCATCCA TTTGACAATG AACATCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1981 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2041 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAACCTGT 2101 AGTTTTCCCT ACCCAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2161 aagatt