Construct: ORF TRCN0000479280

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018540.1_s317c1
Derived from:
ccsbBroadEn_11545
DNA Barcode:
AAACCTGTAGTTTTCCCTACCCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPN9 (10753)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479280
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10753 CAPN9 calpain 9 NM_001319676.1 99.9% 99.8% 775A>C
2 human 10753 CAPN9 calpain 9 NM_006615.3 90.8% 90.7% 213_401del;964A>C
3 human 10753 CAPN9 calpain 9 XM_011544019.2 89.5% 89.7% 775A>C;1798_1799ins59;1823_1974del
4 human 10753 CAPN9 calpain 9 NM_016452.2 87% 86.8% 213_401del;874_875ins78;886A>C
5 human 10753 CAPN9 calpain 9 XM_011544017.1 81.9% 82% (many diffs)
6 human 10753 CAPN9 calpain 9 XM_017000099.1 78.8% 78.7% 0_1ins396;379A>C
7 human 10753 CAPN9 calpain 9 XM_011544018.1 78.4% 78.3% (many diffs)
8 human 10753 CAPN9 calpain 9 XM_017000098.1 78.1% 78.2% (many diffs)
9 human 10753 CAPN9 calpain 9 XR_001736933.1 73.5% (many diffs)
10 human 10753 CAPN9 calpain 9 XM_011544020.1 70% 70% (many diffs)
11 human 10753 CAPN9 calpain 9 XR_949127.1 61.7% (many diffs)
12 human 10753 CAPN9 calpain 9 XM_024452513.1 51.9% 51.7% (many diffs)
13 human 10753 CAPN9 calpain 9 XM_024452514.1 51.9% 51.7% (many diffs)
14 mouse 73647 Capn9 calpain 9 NM_023709.4 75.5% 77.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1947
ORF length:
1881
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc ttacctctac cgggccccag ggcctcaggc acacccggtt cccaaggacg
121cccggatcac ccactcctca ggccagagct ttgagcaaat gaggcaggag tgcctgcaga
181gaggcaccct gtttgaggat gcagacttcc cagccagcaa ttcctccctg ttctacagtg
241agaggccgca gatccccttt gtgtggaaac gaccagggtt ctggcagcac agtgagtggc
301tggacgtggt gatcgatgac cgcctgccca ccttcaggga ccgcttggtt ttcctccact
361ctgccgacca caacgagttc tggagcgcct tgctggaaaa agcctacgcc aagctaaatg
421ggagctatga agctctgaag ggaggcagcg ccatcgaggc catggaagac ttcactgggg
481gtgtggcaga gaccttccaa actaaagagg cccccgagaa cttctatgag attctagaga
541aggctttgaa gagaggctcc ctgctgggct gcttcattga taccagaagt gctgcagaat
601ctgaggcccg gacgccgttt ggtcttatta agggtcatgc ctacagtgta acgggaattg
661accaggtaag cttccgaggc cagagaatcg agctcatccg aatccggaac ccttggggcc
721aggttgagtg gaacgggtcg tggagcgaca gttctccgga gtggcgttct gttggtccag
781ctgagcagaa gcgtctgtgt cacactgctc tggatgatgg ggaattctgg atggcatttc
841aggacttcaa ggcccacttt gataaagtgg agatctgcaa cctcactccc gatgccctgg
901aggaagacgc gatccacaaa tgggaggtga cggtccatca gggaagctgg gttcgcggct
961ccacggctgg gggctgccgc aatttcctgg ataccttttg gaccaatcca caaataaaat
1021tgtctctgac tgagaaagat gaggggcagg aggagtgtag tttccttgta gccctgatgc
1081agaaagatag aaggaaactc aagagatttg gtgccaatgt gctgacaatc ggctatgcca
1141tttatgagtg ccctgacaaa gacgaacacc tgaacaaaga cttcttcaga taccacgctt
1201ctcgggccag aagcaagacg ttcatcaacc tgagagaagt ctccgaccgg ttcaagctgc
1261cccctgggga gtacatcctg attcccagca cttttgagcc ccaccaggaa gctgatttct
1321gtctgagaat cttttcagag aaaaaagcca ttacccggga tatggatgga aatgtagaca
1381ttgaccttcc tgagcctcca aagccaactc cacctgacca ggagacagag gaggagcagc
1441ggtttcgggc tctgtttgaa caagtcgctg gtgaggacat ggaggtgaca gcagaggaac
1501ttgagtatgt tttaaatgct gtgctgcaaa agaaaaagga catcaaattc aagaagctaa
1561gcctgatctc ctgtaaaaac atcatttccc TGATGGACAC CAGCGGCAAT GGGAAGCTGG
1621AGTTTGATGA ATTCAAAGTG TTCTGGGACA AGCTGAAGCA GTGGATTAAC CTTTTCCTTC
1681GGTTTGATGC TGACAAGTCC GGCACCATGT CTACCTATGA ACTACGGACT GCACTGAAAG
1741CTGCAGGCTT TCAGCTGAGC AGCCACCTCC TGCAGCTGAT TGTGCTCAGG TATGCGGATG
1801AGGAGCTCCA GCTGGACTTC GATGACTTCC TCAACTGCCT GGTCCGGCTG GAGAATGCGA
1861GCCGGGTGTT CCAGGCTCTC AGTACAAAGA ACAAGGAGTT CATTCATCTC AATATAAATG
1921AGTTCATCCA TTTGACAATG AACATCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
1981GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2041AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAACCTGT
2101AGTTTTCCCT ACCCAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2161aagatt

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