Construct: ORF TRCN0000479285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017162.1_s317c1
- Derived from:
- ccsbBroadEn_03934
- DNA Barcode:
- GTTATTAAATGATCAGACCCAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P3H1 (64175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | NM_001146289.1 | 100% | 100% | |
| 2 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | NM_022356.4 | 93% | 92.8% | 2048_2049insCGAGCGGGTGAGAGCAGCT;2073_2208del |
| 3 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | NM_001243246.1 | 86.5% | 86% | (many diffs) |
| 4 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | XR_946739.2 | 86.3% | 1_57del;1281_1348del;2039_2040ins177 | |
| 5 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | XM_005271110.2 | 47.7% | 47.4% | 0_1ins1008;1040_1041insCGAGCGGGTGAGAGCAGCT;1065_1200del |
| 6 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | XM_017002052.2 | 45.7% | 43.7% | (many diffs) |
| 7 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | XM_011541948.1 | 45.6% | 43.8% | (many diffs) |
| 8 | human | 64175 | P3H1 | prolyl 3-hydroxylase 1 | XM_017002051.2 | 45.6% | 43.8% | (many diffs) |
| 9 | mouse | 56401 | P3h1 | prolyl 3-hydroxylase 1 | NM_019783.2 | 81.5% | 82.2% | (many diffs) |
| 10 | mouse | 56401 | P3h1 | prolyl 3-hydroxylase 1 | NM_001286148.1 | 80.7% | 81.3% | (many diffs) |
| 11 | mouse | 56401 | P3h1 | prolyl 3-hydroxylase 1 | NM_019782.3 | 80.5% | 81.2% | (many diffs) |
| 12 | mouse | 56401 | P3h1 | prolyl 3-hydroxylase 1 | NM_001042411.1 | 62.2% | 64.5% | (many diffs) |
| 13 | mouse | 56401 | P3h1 | prolyl 3-hydroxylase 1 | XR_001784167.1 | 55.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2160
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggtacgc gcgttgaagc tgctgaccac actgctggct gtcgtggccg 121 ctgcctccca agccgaggtc gagtccgagg caggatgggg catggtgacg cctgatctgc 181 tcttcgccga ggggaccgca gcctacgcgc gcggggactg gcccggggtg gtcctgagca 241 tggaacgggc gctgcgctcc cgggcagccc tccgcgccct tcgcctgcgc tgccgcaccc 301 agtgtgccgc cgacttcccg tgggagctgg accccgactg gtcccccagc ccggcccagg 361 cctcgggcgc cgccgccctg cgcgacctga gcttcttcgg gggccttctg cgtcgcgctg 421 cctgcctgcg ccgctgcctc gggccgccgg ccgcccactc gctcagcgaa gagatggagc 481 tggagttccg caagcggagc ccctacaact acctgcaggt cgcctacttc aagatcaaca 541 agttggagaa agctgttgct gcagcacaca ccttcttcgt gggcaatcct gagcacatgg 601 aaatgcagca gaacctagac tattaccaaa ccatgtctgg agtgaaggag gccgacttca 661 aggatcttga gactcaaccc catatgcaag aatttcgact gggagtgcga ctctactcag 721 aggaacagcc acaggaagct gtgccccacc tagaggcggc gctgcaagaa tactttgtgg 781 cctatgagga gtgccgtgcc ctctgcgaag ggccctatga ctacgatggc tacaactacc 841 ttgagtacaa cgctgacctc ttccaggcca tcacagatca ttacatccag gtcctcaact 901 gtaagcagaa ctgtgtcacg gagcttgctt cccacccaag tcgagagaag ccctttgaag 961 acttcctccc atcgcattat aattatctgc agtttgccta ctataacatt gggaattata 1021 cacaggctgt tgaatgtgcc aagacctatc ttctcttctt ccccaatgac gaggtgatga 1081 accaaaattt ggcctattat gcagctatgc ttggagaaga acacaccaga tccatcggcc 1141 cccgtgagag tgccaaggag taccgacagc gaagcctact ggaaaaagaa ctgcttttct 1201 tcgcttatga tgtttttgga attccctttg tggatccgga ttcatggact ccagaagaag 1261 tgattcccaa gagattgcaa gagaaacaga agtcagaacg ggaaacagcc gtacgcatct 1321 cccaggagat tgggaacctt atgaaggaaa tcgagaccct tgtggaagag aagaccaagg 1381 agtcactgga tgtgagcaga ctgacccggg aaggtggccc cctgctgtat gaaggcatca 1441 gtctcaccat gaactccaaa ctcctgaatg gttcccagcg ggtggtgatg gacggcgtaa 1501 tctctgacca cgagtgtcag gagctgcaga gactgaccaa tgtggcagca acctcaggag 1561 atggctaccg gggtcagacc tccccacata ctcccaatga aaagttctat ggtgtcactg 1621 tcttcaaagc cctcaagctg gggcaagaag gcaaagttcc tctgcagagt gcccacctgt 1681 actacaacgt gacggagaag gtgcggcgca tcatggagtc ctacttccgc ctggatacgc 1741 ccctctactt ttcctactcT CATCTGGTGT GCCGCACTGC CATCGAAGAG GTCCAGGCAG 1801 AGAGGAAGGA TGATAGTCAT CCAGTCCACG TGGACAACTG CATCCTGAAT GCCGAGACCC 1861 TCGTGTGTGT CAAAGAGCCC CCAGCCTACA CCTTCCGCGA CTACAGCGCC ATCCTTTACC 1921 TAAATGGGGA CTTCGATGGC GGAAACTTTT ATTTCACTGA ACTGGATGCC AAGACCGTGA 1981 CGGCAGAGGT GCAGCCTCAG TGTGGAAGAG CCGTGGGATT CTCTTCAGGC ACTGAAAACC 2041 CACATGGAGT GAAGGCTGTC ACCAGGGGGC AGCGCTGTGC CATCGCCCTG TGGTTCACCC 2101 TGGACCCTCG ACACAGCGAG CGGGTGAGAG CAGCTCGAGC GGGACAGGGT GCAGGCAGAT 2161 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2281 TTTATATATC TTGTGGAAAG GACGAGTTAT TAAATGATCA GACCCAAGTA CGCGTTAAGT 2341 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt