Construct: ORF TRCN0000479297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014761.1_s317c1
- Derived from:
- ccsbBroadEn_07925
- DNA Barcode:
- TAGCCATTATAACCGGATCCTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEMIP2 (23670)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479297
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23670 | CEMIP2 | cell migration inducing hya... | NM_013390.3 | 99.9% | 99.9% | 3928G>T |
| 2 | human | 23670 | CEMIP2 | cell migration inducing hya... | XM_005251869.4 | 99.9% | 99.9% | 3928G>T |
| 3 | human | 23670 | CEMIP2 | cell migration inducing hya... | NM_001135820.2 | 95.4% | 95.3% | 1201_1202ins189;3739G>T |
| 4 | human | 23670 | CEMIP2 | cell migration inducing hya... | NM_001349784.2 | 53.8% | 53.7% | 0_1ins1914;2014G>T |
| 5 | mouse | 83921 | Tmem2 | transmembrane protein 2 | NM_001033759.2 | 84.5% | 86.6% | (many diffs) |
| 6 | mouse | 83921 | Tmem2 | transmembrane protein 2 | NM_031997.4 | 84.5% | 86.6% | (many diffs) |
| 7 | mouse | 83921 | Tmem2 | transmembrane protein 2 | XM_006527486.3 | 84.5% | 86.6% | (many diffs) |
| 8 | mouse | 83921 | Tmem2 | transmembrane protein 2 | XM_006527487.3 | 84.5% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4218
- ORF length:
- 4149
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtatgccact gattccaggg gacactcccc tgctttcctc caacctcaga 121 atggaaatag tcgtcaccca tctggctatg ttccagggaa ggttgtccca ttgcgtcccc 181 ctcctcctcc aaagagtcaa gcttcagcca aatttacctc catcagacga gaagaccggg 241 caaccttcgc attctcacct gaagaacagc aagcccagag agaaagtcaa aagcaaaaga 301 gacacaaaaa tactttcatt tgttttgcta ttactagttt ctcatttttt attgcacttg 361 caatcatttt aggaatatcc tcaaaatatg ctccagatga aaattgccca gatcaaaatc 421 ctcgtctcag gaattgggat ccaggacaag attctgcaaa gcaagttgtt atcaaggagg 481 gagatatgct ccgtctgacc tcagacgcca ccgtgcattc tatagtcatt caggatggag 541 gactgcttgt atttggggac aataaagatg gatccagaaa tattactttg aggactcatt 601 acatcctgat ccaggatggt ggggcgcttc atattggagc agaaaaatgc cgctataaat 661 ccaaagcgac aattaccttg tatggcaagt cagatgaagg tgaaagtatg ccaacatttg 721 gcaaaaagtt tattggtgtg gaagctggcg ggacactgga gttacatggg gcacggaagg 781 catcgtggac gttgttggca aggaccctga attcctcagg cttgcccttt gggtcctata 841 cctttgaaaa ggacttttcc cggggcctca atgtgagggt cattgaccaa gacacggcca 901 aaattttgga aagtgagaga tttgataccc atgaataccg caatgagagc aggcggcttc 961 aggagtttct gagattccag gatccaggtc ggattgttgc catagctgtc ggggattcag 1021 ccgctaaaag tctcttacaa ggaaccatcc agatgatcca ggaacggttg ggaagtgaac 1081 tgatccaagg actgggctac aggcaagctt gggctttagt tggtgtcatt gatggtggaa 1141 gcacttcttg caatgaatcc gtgagaaact atgaaaatca tagcagtggc gggaaggctc 1201 ttgcccaaag agaattttat actgtggatg gccagaagtt ctctgtgaca gcttatagtg 1261 aatggattga aggcgtttct ctttcaggat tccgggtaga ggttgtagat ggagtgaagc 1321 taaatttgct agatgatgtt agtagttgga aacctggaga ccagattgtg gtcgcaagca 1381 cagactattc catgtaccaa gcagaggagt tcactcttct tccctgttct gaatgcagcc 1441 attttcaggt caaagtcaaa gaaacccctc agttcctgca catgggtgag atcatagacg 1501 gtgtagacat gagagctgag gttggaattc ttacccggaa tattgtgatc caaggagaag 1561 tggaggactc atgctacgca gaaaatcagt gccaattttt tgattatgat acctttgggg 1621 gacacattat gataatgaaa aattttactt cagtccatct ttcttatgtg gaattgaaac 1681 acatgggtca gcagcagatg gggcgatacc ctgttcattt tcacctgtgt ggtgacgtgg 1741 attataaagg aggatacaga catgcaacat ttgtggacgg cctgtctatt catcacagct 1801 tctcaaggtg catcactgtg catgggacaa atggcttgct aataaaagac accattgggt 1861 ttgacacact aggtcattgt ttctttttgg aagatggtat tgaacagagg aatactttgt 1921 tccacaatct gggactcctc accaagccgg gtactctcct gcccaccgat aggaacaact 1981 ccatgtgtac caccatgcga gataaagtgt ttggaaatta cattcctgtg cctgctactg 2041 actgtatggc tgtttcaact ttctggattg ctcatcccaa caataatctg attaataatg 2101 cagctgcagg ctcacaggat gctggaatat ggtatttatt ccacaaggaa ccaactgggg 2161 aatccagtgg attgcagctc ttggcaaaac cagaactcac tccattgggt atattttata 2221 acaacagggt ccattcaaat tttaaggctg gcttatttat tgacaaaggt gtcaaaacaa 2281 ccaactctag tgctgctgac ccaagggaat acctctgttt ggacaatagt gcaagatttc 2341 gacctcatca ggatgcaaac cccgaaaaac cacgtgttgc tgctctaatt gacaggctca 2401 ttgcttttaa aaataatgat aatggagctt gggtcagagg aggagatatt atcgttcaaa 2461 attcagcatt tgcagataat ggaataggac tgacctttgc cagtgatgga agcttcccaa 2521 gtgatgaagg ttccagccaa gaggtatctg aatctctctt tgttggggag agcaggaatt 2581 acggctttca gggtggtcag aacaagtatg taggcactgg aggaatagac cagaagcctc 2641 gaacattacc caggaacagg acgttcccaa ttagaggctt tcagatttat gatgggccca 2701 ttcatctcac aaggagcact ttcaaaaaat atgtgccaac tccagatagg tacagcagtg 2761 caattggctt cctcatgaag aattcctggc agataacccc caggaataat atctccctcg 2821 tgaagtttgg tccacatgtc tctctgaatg tcttttttgg aaagcctggt ccctggtttg 2881 aagattgtga gatggatggt gataagaact ccatattcca tgacattgat ggctctgtga 2941 caggatacaa ggatgcttat gtgggaagaa tggacaacta cctgatccgc catccaagct 3001 gtgtaaatgt gtctaagtgg aatgcagtga tctgcagtgg gacctatgca caggtctatg 3061 tacagacatg gagcactcag aatctttcta tgaccattac acgagatgag tatccgtcca 3121 accctatggt gctccgaggt attaatcaga aggctgcctt tccacagtac cagcctgtcg 3181 tcatgctgga gaagggttat accatccact ggaatgggcc ggcaccacgg actacatttc 3241 tatacctcgt caacttcaac aagaatgact ggattcgagt tggcctttgc tatccatcaa 3301 acacaagttt tcaagttacc tttggctatt tgcagcggca gaatggctca ttatccaaaa 3361 tcgaagaata tgagcctgtg cattcactgg aagaactgca aagaaagcaa tccgagagga 3421 aattctattt tgactccagc acggggttac tgtttttgta tctcaaagcc aaaagccaca 3481 ggcatggcca cagttactgt tcatctcagg gatgtgaaag agtcaagatc caagcagcca 3541 cagactcaaa ggacatcagt aactgcatgg ccaaagcata cccacagtac tacagaaagc 3601 cgtcagtggt caagcggatg ccggccatgc tcactggact ctgtcaaggc tgtggcactc 3661 ggcaggtggt gtttactagt gatcctcata aaagttacct ccctgtgcaa ttccagtcac 3721 ctgataaagc agaaacccag cgtggagacc cgtctgttat ttctgtcaat ggcactgact 3781 ttaccttccg aagtgcaggc gtccTCCTCC TTGTTGTGGA TCCGTGCAGC GTTCCATTCC 3841 GCTTGACGGA AAAAACGGTT TTTCCTCTTG CTGATGTCAG TCGCATTGAA GAGTATTTAA 3901 AAACAGGCAT CCCTCCAAGG TCCATTGTTC TGTTGAGCAC AAGAGGAGAA ATAAAGCAGT 3961 TAAACATTTC ACACTTACTA GTACCTCTGG GATTATCCAA ACCAGCTCAT CTTTATGACA 4021 AAGGGAGTAC CATATTTTTG GGATTCAGTG GAAACTTTAA ACCATCATGG ACTAAGCTAT 4081 TTACCAGTCC TGCTGGACAG GGCCTTGGGG TGCTTGAACA ATTCATACCT TTGCAGCTGG 4141 ACGAATATGG TTGTCCCAGA GCCACCACTG TCCGCAGAAG AGACCTGGAA CTGCTAAAGC 4201 AAGCTTCAAA AGCACATTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 4261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 4321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAGCCAT TATAACCGGA 4381 TCCTCGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt