Construct: ORF TRCN0000479299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004852.1_s317c1
- Derived from:
- ccsbBroadEn_07483
- DNA Barcode:
- ATATGACTAGACACAAACCTCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA0232 (9778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479299
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9778 | KIAA0232 | KIAA0232 | NM_001100590.1 | 99.9% | 99.8% | 1605T>A;1860C>A |
| 2 | human | 9778 | KIAA0232 | KIAA0232 | NM_014743.3 | 99.9% | 99.8% | 1605T>A;1860C>A |
| 3 | human | 9778 | KIAA0232 | KIAA0232 | XM_017008860.2 | 98.2% | 98.1% | 1605T>A;1860C>A;3802_3873del |
| 4 | human | 9778 | KIAA0232 | KIAA0232 | XM_024454289.1 | 98.2% | 98.1% | 1605T>A;1860C>A;3802_3873del |
| 5 | human | 9778 | KIAA0232 | KIAA0232 | XM_024454291.1 | 93.7% | 93.2% | (many diffs) |
| 6 | human | 9778 | KIAA0232 | KIAA0232 | XM_017008861.1 | 92.1% | 91.6% | (many diffs) |
| 7 | human | 9778 | KIAA0232 | KIAA0232 | XM_011513598.3 | 90.7% | 90.6% | (many diffs) |
| 8 | human | 9778 | KIAA0232 | KIAA0232 | XM_024454290.1 | 90.7% | 90.6% | (many diffs) |
| 9 | human | 9778 | KIAA0232 | KIAA0232 | XM_005248041.5 | 90.1% | 89.5% | (many diffs) |
| 10 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | NM_001081232.3 | 88.1% | 88.2% | (many diffs) |
| 11 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240748.2 | 87.6% | 87.7% | (many diffs) |
| 12 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240747.2 | 86.8% | 86.9% | (many diffs) |
| 13 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_006503992.2 | 86.7% | 86.8% | (many diffs) |
| 14 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240742.2 | 86.2% | 86.3% | (many diffs) |
| 15 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240743.2 | 86.2% | 86.3% | (many diffs) |
| 16 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240744.2 | 86.2% | 86.3% | (many diffs) |
| 17 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240745.2 | 86.2% | 86.3% | (many diffs) |
| 18 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240746.2 | 86.2% | 86.3% | (many diffs) |
| 19 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240750.2 | 82.3% | 82.5% | (many diffs) |
| 20 | mouse | 320661 | D5Ertd579e | DNA segment, Chr 5, ERATO D... | XM_011240749.2 | 81% | 81.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4254
- ORF length:
- 4185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtaccctatc tgtacagttg ttgtggatgg tttgccatct gaaagctcct 121 caagttctta tccaggccct gtgtctgttt ctgaaatgtc tctgcttcat gctttgggtc 181 cagtgcagac ctggctggga caagagctcg agaaatgtgg cattgatgcc atgatttaca 241 ctcggtatgt cctcagtctt ctgctgcatg acagctatga ctacgacctg caggaacagg 301 agaatgacat cttcctgggc tgggaaaaag gagcttataa gaaatgggga aagagtaaga 361 aaaaatgttc agatctaact ctagaagaaa tgaaaaaaca ggctgctgtc cagtgtcttc 421 gatctgcttc tgatgaaagc tctggtatcg agactttagt ggaggagctc tgctccagac 481 tgaaagacct tcagagtaag caagaagaga agattcacaa aaagttagag gggtctccct 541 ctccagaggc agaattatcc cctccagcaa aggatcaagt ggaaatgtac tatgaagcat 601 ttccaccact ttctgagaaa ccagtttgcc tgcaagaaat catgactgtg tggaacaagt 661 ctaaagtctg ttcttactct agctcttctt catcatccac agccccacca gctagcacag 721 atacttcctc tcctaaggac tgcaacagtg aaagtgaagt caccaaggaa agaagcagtg 781 aagtacccac cactgtgcat gagaaaaccc agagcaaaag caaaaacgag aaggaaaaca 841 aatttagtaa tggcacaatt gaagaaaagc ctgctttgta caaaaagcaa atccgacata 901 aacctgaagg aaagattcgc cctcgctcgt ggtcttctgg ctccagtgaa gcaggctcaa 961 gttccagtgg gaatcaggga gaattaaaag catccatgaa gtatgttaaa gtaagacaca 1021 aggcacgaga gattcgaaac aaaaaagggc ggaatgggca aagcaggctt tctttgaagc 1081 acggtgaaaa ggctgaaagg aacattcata ctggaagtag tagcagtagc agcagtggtt 1141 ctgtcaaaca gctgtgcaag cggggtaaga gacctttaaa agaaataggg agaaaagatc 1201 ctgggagcac tgaaggaaaa gacctgtaca tggagaatag aaaggacaca gagtataaag 1261 aggagccctt gtggtacacc gagccaattg ctgaatattt tgttcctctg agcagaaaaa 1321 gtaaactaga gaccacatac cgaaacagac aggatacaag tgatctgaca tcagaggcag 1381 tggaagaatt gtctgaatca gtgcatggtc tttgtatcag caacaataat cttcataaaa 1441 catacctcgc agcaggtact ttcattgatg gtcattttgt agaaatgcct gcagttataa 1501 atgaggatat tgacctcact gggacctcat tatgttctct accagaggac aataaatacc 1561 tggatgatat tcatctatca gaattaacgc acttctatga agtggatatt gatcaatcca 1621 tgttggatcc tggtgcctca gaaacaatgc aaggagaaag tcggattttg aaaatgattc 1681 gacagaaaag caaagagaac acagattttg aggcagaatg ttgcatagtg ttagatggta 1741 tggagttgca aggggaacgt gcaatatgga cagattctac cagctccgta ggtgctgagg 1801 gcttattcct gcaggacctt ggcaatctgg ctcagttttg ggagtgctgt tcatccagct 1861 ccggtgatgc tgatggggag agttttggag gagactctcc agttagactc tctcccatct 1921 tagacagaac agtgctcaat tcacacctgc ttgctggcaa tcaagagctc ttttcagata 1981 ttaatgaagg atctggtata aactcttgtt tttcagtgtt tgaagtgcaa tgcagtaatt 2041 ctgttttacc attttctttt gaaacactca acttgggaaa tgaaaataca gattctagtg 2101 ctaatatgct tgggaaaaca cagtctagat tgctaatatg gaccaaaaat agtgcctttg 2161 aagaaaatga acactgttct aatctttcaa caagaacttg tagtccatgg tcccattcag 2221 aagaaacacg ttcagacaat gaaacattaa atattcagtt tgaagaatcc acacagttta 2281 atgccgaaga tattaattat gtagttccta gagtctcgtc aaattatgta gatgaagaac 2341 ttctagattt tttgcaagat gaaacttgcc agcaaaacag tagaacttta ggtgagattc 2401 ctacattagt tttcaaaaaa acatctaaac tagaatccgt ctgtggtatt cagctagaac 2461 aaaaaacaga aaacaaaaat tttgaaacta cacaagtatg taatgaaagt ccacatggag 2521 atggctacag ctcaggggtt attaaagaca tttggacaaa gatggcagac acaaattctg 2581 tggctacagt agaaatagaa agaactgatg ctgagttgtt ttcggcagat gtaaataact 2641 actgctgctg tctagatgct gaagctgaac tggagaccct tcaggagcct gataaggctg 2701 tgcggaggtc agagtaccat ctgtgggagg gacagaaaga gagcctggag aaaagagcat 2761 ttgcttctag tgagctatca aacgtggatg gtggtgatta tacaacaccc tctaaaccct 2821 gggatgtagc ccaagataaa gaaaacacat tcattcttgg aggagtttat ggagaactca 2881 aaaccttcaa tagtgatggg gagtgggcag tcgtaccacc tagtcacaca aaaggaagtc 2941 tgttacagtg tgcagcttct gatgttgtga cgatagctgg tacagatgtc tttatgaccc 3001 caggaaacag ttttgctcct gggcacaggc agttatggaa acccttcgtg tcatttgaac 3061 agaatgatca gccgaagagt ggggaaaatg ggttaaataa gggattttct tttatcttcc 3121 atgaagactt actaggagct tgtggcaact ttcaagtcga agatcctgga cttgaatact 3181 cattttcttc ctttgactta agcaatccat tttcacaagt tcttcatgta gaatgctcat 3241 ttgaacctga agggattgca tctttcagcc ccagttttaa accgaaatca atcctctgtt 3301 ctgattcaga cagtgaagtg tttcacccca ggatatgtgg tgttgacaga acacaataca 3361 gggctattcg gatctctcct cggactcact ttcgcccaat ttctgcatcc gaactgtccc 3421 caggaggagg aagcgagtca gaatttgaat ctgagaaaga tgaagcaaat attcccattc 3481 cttctcaagt tgatatattt gaagatccgc aggcagatct caaacctttg gaagaagatg 3541 cagagaaaga aggccattac tatggaaaat cagagcttga gtctggaaaa ttccttccca 3601 ggttaaaaaa atctgggatg gaaaagagtg ctcagacatc actggattcc caggaggaat 3661 caactgggat tctttcagta ggaaagcaaa atcagtgttt ggaatgtagc atgaatgaat 3721 ccctggaaat agatttagaa agctcagaag caaattgtaa aataatggca caatgcgagg 3781 aagaaattaa taatttttgt ggttgcaaag caggttgtca gtttcctgct tatgaagata 3841 atccagtttc TTCGGGACAG CTGGAAGAGT TCCCTGTATT GAACACTGAT ATACAAGGAA 3901 TGAATAGAAG TCAAGAAAAA CAGACCTGGT GGGAAAAAGC CTTGTACTCT CCTCTTTTTC 3961 CTGCATCAGA GTGTGAAGAA TGTTACACAA ATGCCAAGGG AGAGAGTGGT TTAGAAGAAT 4021 ATCCAGATGC TAAAGAGACA CCCAGTAATG AAGAGCGCCT GTTAGATTTT AATAGGGTGT 4081 CTTCTGTTTA TGAAGCAAGA TGTACAGGAG AGAGAGATTC TGGAGCAAAG TCAGATGGCT 4141 TCCGCGGAAA GATGTGCTCC AGCGCCAGCT CCACCTCGGA AGAGACAGGC TCAGAAGGCG 4201 GAGGCGAGTG GGTGGGCCCT AGTGAAGAGG AGCTCTTTTC TCGAACTCAT CTCTTGCCAA 4261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 4321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 4381 TATCTTGTGG AAAGGACGAA TATGACTAGA CACAAACCTC CTAACGCGTT AAGTCgacaa 4441 tcaacctctg gattacaaaa tttgtgaaag att