Construct: ORF TRCN0000479303
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001595.1_s317c1
- Derived from:
- ccsbBroadEn_00580
- DNA Barcode:
- CACAGAAACCATGCTGTTGTAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLT1 (2321)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479303
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_001159920.2 | 100% | 100% | |
| 2 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | XM_011535014.1 | 93.6% | 90.4% | (many diffs) |
| 3 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_001160030.2 | 91.5% | 88.8% | (many diffs) |
| 4 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_001160031.1 | 78% | 75.3% | (many diffs) |
| 5 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | XM_017020485.1 | 52.2% | 50.4% | (many diffs) |
| 6 | human | 2321 | FLT1 | fms related tyrosine kinase 1 | NM_002019.4 | 50.7% | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2130
- ORF length:
- 2061
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcagctac tgggacaccg gggtcctgct gtgcgcgctg ctcagctgtc 121 tgcttctcac aggatctagt tcaggttcaa aattaaaaga tcctgaactg agtttaaaag 181 gcacccagca catcatgcaa gcaggccaga cactgcatct ccaatgcagg ggggaagcag 241 cccataaatg gtctttgcct gaaatggtga gtaaggaaag cgaaaggctg agcataacta 301 aatctgcctg tggaagaaat ggcaaacaat tctgcagtac tttaaccttg aacacagctc 361 aagcaaacca cactggcttc tacagctgca aatatctagc tgtacctact tcaaagaaga 421 aggaaacaga atctgcaatc tatatattta ttagtgatac aggtagacct ttcgtagaga 481 tgtacagtga aatccccgaa attatacaca tgactgaagg aagggagctc gtcattccct 541 gccgggttac gtcacctaac atcactgtta ctttaaaaaa gtttccactt gacactttga 601 tccctgatgg aaaacgcata atctgggaca gtagaaaggg cttcatcata tcaaatgcaa 661 cgtacaaaga aatagggctt ctgacctgtg aagcaacagt caatgggcat ttgtataaga 721 caaactatct cacacatcga caaaccaata caatcataga tgtccaaata agcacaccac 781 gcccagtcaa attacttaga ggccatactc ttgtcctcaa ttgtactgct accactccct 841 tgaacacgag agttcaaatg acctggagtt accctgatga aaaaaataag agagcttccg 901 taaggcgacg aattgaccaa agcaattccc atgccaacat attctacagt gttcttacta 961 ttgacaaaat gcagaacaaa gacaaaggac tttatacttg tcgtgtaagg agtggaccat 1021 cattcaaatc tgttaacacc tcagtgcata tatatgataa agcattcatc actgtgaaac 1081 atcgaaaaca gcaggtgctt gaaaccgtag ctggcaagcg gtcttaccgg ctctctatga 1141 aagtgaaggc atttccctcg ccggaagttg tatggttaaa agatgggtta cctgcgactg 1201 agaaatctgc tcgctatttg actcgtggct actcgttaat tatcaaggac gtaactgaag 1261 aggatgcagg gaattataca atcttgctga gcataaaaca gtcaaatgtg tttaaaaacc 1321 tcactgccac tctaattgtc aatgtgaaac cccagattta cgaaaaggcc gtgtcatcgt 1381 ttccagaccc ggctctctac ccactgggca gcagacaaat cctgacttgt accgcatatg 1441 gtatccctca acctacaatc aagtggttct ggcacccctg taaccataat cattccgaag 1501 caaggtgtga cttttgttcc aataatgaag agtcctttat cctggatgct gacagcaaca 1561 tgggaaacag aattgagagc atcactcagc gcatggcaat aatagaagga aagaataaga 1621 tggctagcac cttggttgtg gctgactcta gaatttctgg aatctacatt tgcatagctt 1681 ccaataaagt tgggactgtg ggaagaaaca taagctttta tatcacagat gtgccaaatg 1741 GGTTTCATGT TAACTTGGAA AAAATGCCGA CGGAAGGAGA GGACCTGAAA CTGTCTTGCA 1801 CAGTTAACAA GTTCTTATAC AGAGACGTTA CTTGGATTTT ACTGCGGACA GTTAATAACA 1861 GAACAATGCA CTACAGTATT AGCAAGCAAA AAATGGCCAT CACTAAGGAG CACTCCATCA 1921 CTCTTAATCT TACCATCATG AATGTTTCCC TGCAAGATTC AGGCACCTAT GCCTGCAGAG 1981 CCAGGAATGT ATACACAGGG GAAGAAATCC TCCAGAAGAA AGAAATTACA ATCAGAGGTG 2041 AGCACTGCAA CAAAAAGGCT GTTTTCTCTC GGATCTCCAA ATTTAAAAGC ACAAGGAATG 2101 ATTGTACCAC ACAAAGTAAT GTAAAACATT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2161 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2221 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACACAG 2281 AAACCATGCT GTTGTAAAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2341 tgaaagatt