Construct: ORF TRCN0000479306
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002194.1_s317c1
- Derived from:
- ccsbBroadEn_14132
- DNA Barcode:
- TTAGTTGTGGATACGCTAGTAAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FAM135B (51059)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479306
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51059 | FAM135B | family with sequence simila... | XM_011517068.2 | 99.8% | (many diffs) | |
| 2 | human | 51059 | FAM135B | family with sequence simila... | XM_011517069.2 | 95.4% | (many diffs) | |
| 3 | human | 51059 | FAM135B | family with sequence simila... | XM_017013471.1 | 93.8% | (many diffs) | |
| 4 | human | 51059 | FAM135B | family with sequence simila... | XM_011517065.1 | 93.6% | (many diffs) | |
| 5 | human | 51059 | FAM135B | family with sequence simila... | NM_001362965.1 | 92.8% | (many diffs) | |
| 6 | human | 51059 | FAM135B | family with sequence simila... | NM_015912.4 | 92.8% | (many diffs) | |
| 7 | human | 51059 | FAM135B | family with sequence simila... | XM_011517064.2 | 92.8% | (many diffs) | |
| 8 | human | 51059 | FAM135B | family with sequence simila... | XM_011517056.2 | 92.5% | (many diffs) | |
| 9 | human | 51059 | FAM135B | family with sequence simila... | XM_011517058.2 | 92.5% | (many diffs) | |
| 10 | human | 51059 | FAM135B | family with sequence simila... | XM_011517059.1 | 92.5% | (many diffs) | |
| 11 | human | 51059 | FAM135B | family with sequence simila... | XM_011517060.2 | 92.5% | (many diffs) | |
| 12 | human | 51059 | FAM135B | family with sequence simila... | XM_011517061.2 | 92.5% | (many diffs) | |
| 13 | human | 51059 | FAM135B | family with sequence simila... | XM_011517062.1 | 92.5% | (many diffs) | |
| 14 | human | 51059 | FAM135B | family with sequence simila... | XM_011517063.2 | 92.5% | (many diffs) | |
| 15 | human | 51059 | FAM135B | family with sequence simila... | XM_011517072.2 | 91.1% | (many diffs) | |
| 16 | human | 51059 | FAM135B | family with sequence simila... | XM_011517073.2 | 89.3% | (many diffs) | |
| 17 | human | 51059 | FAM135B | family with sequence simila... | XM_011517074.1 | 86.7% | (many diffs) | |
| 18 | human | 51059 | FAM135B | family with sequence simila... | XM_011517070.2 | 78.9% | (many diffs) | |
| 19 | human | 51059 | FAM135B | family with sequence simila... | XR_001745532.1 | 50.9% | (many diffs) | |
| 20 | human | 51059 | FAM135B | family with sequence simila... | XR_001745531.1 | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 81
- ORF length:
- 12
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagacgac tgagtgaagt agattttcaa ctcaaggtgg atctgcactt 121 tacggacagt gaacagcagt tgagggatgt ggctggggca ccgatggtca gcagccgaac 181 gcttggcctg cacttccacc cccggaatgg tctgcaccac caggtcccgg tcatgttcga 241 ctatttccac ctgtctgtga tctcggtgac cgtccatgct gccctggtgg ctctgcagca 301 gccattgatc agttttactc gtccaggaag aggctcctgg cttggtaaag gtggcccaga 361 caccggacaa gaacagtcta tcatttctct ggaaaacttg gtctttggag ctgggtactg 421 caagccgact tcctcagagg gaagcttcta catcacctct gagaactgca tgcagcacgc 481 acacaagtgg caccgagacc tgtggctgtt gctcctccac gcttaccggg gtctccgtct 541 ccacttcctg gtgatcatgc gggacatccc agagctgcca cacacggagc tggaggccct 601 tgctgttgaa gaaacacttt ctcagctgtg ctcagagcta cagatgttga acaatccaga 661 gaagatcgct gagcagataa gcaaggatct ggcctggctc acgtcccaca tgatgactct 721 gtggacccag ttcctggaca cagtcactct gcactcccaa gtgaccactt atctcaccca 781 ggaacaccac accctgaggg tccgaaggtt ttctgaggcc ttcttttaca tggagcacca 841 aaaacttgca gtcctgacat ttcaggagaa tctgatacag acgcacagcc agctgtccct 901 ggatatccgg aactcggagt acctcactag catgcccccg ctgcctgcag agtgcctgga 961 catcgacggc gattggaaca ccctgccggt gatctttgag gacagatacg tggactgccc 1021 tgcgacaggg cataacttga gtgtttatcc taattttgat gttccagtga caagtcctac 1081 aataatgaat ctgaaagaca aggaagataa ctgtatggta aatagcaatt tatcttttag 1141 ggaagacctt gtcttgtcta ccataaaacc atcccaaatg gattctgatg aagaagttgt 1201 aaggtgtcca gagccgggtg agaatgtggc cacacaaaat catatggaca tgtgctctga 1261 atctcaggtg tatatatcaa ttggtgaatt tcaaaacaaa gcaggtgtgc ctgaagatga 1321 atgttggact ggccaaacat ctgatgctgg gacatatcca gtggcagatg tggatacttc 1381 tagaaggagt ccaggtccag aggatggaca ggccccagtg ctgacctaca ttgacgtaaa 1441 atctagcaat aagaacccct ccagagctga acccctggtg gccttcaatg ctcagcatga 1501 gagtaggagc tctagagata agtatggatt agacaggact gggctaagca aagtggtagt 1561 aggtggaagc caccaaaatg ccatctcttc agacaaaaca actctccatg aattaagtac 1621 tctaggaaag ggaatagatc aagaggggaa gatggtgctg ctaagcttga aactcacccc 1681 ctctgagccc tgtgatccac taagttctac cctgagggag cccttagata ttaggtcttc 1741 cctaaaggac tctcacacag aagagcagga ggaactctca gtgctatccg gggtcatcaa 1801 gagatcttca tccatcatat ctgattcagg cattgagagt gagccaagct ccgtcgcctg 1861 gtcagaggcc cgaagcaggg ctctggagtt gcccagtgat cgggaagtct tgcacccgtt 1921 tgttcgaaga catgccctcc accggaactc cctagagggt ggacacacag aaagtaacac 1981 aagtttgcca agcggcatcc aggcttctct cacctccatt agctctttac cttttgagga 2041 ggatgagcgg gaggtggcac tcactaagtt aaccaagtct gtatctgctc cccacatcag 2101 tagcccagag gaggctgctg aagatgcgga caccaagcag caagatggag gttttgctga 2161 accttcagat atgcacagca agagccaagg ttccccagga tcttgctctc aactttgtgg 2221 tgactctgga acagatgctg gagcagacca tcccctggtg gagatagttt tagatgctga 2281 caaccagcag ggccccggat acatagacat ccccaaaggg aaagggaagc agtttgatgc 2341 tcaaggacac tgtcttcctg atggcaggac tgagaacact ccaggtgttg aaaccaaagg 2401 tcttaattta aaaataccac gcgtcatagc acttgaaaac cccaggacca gatctcttca 2461 tagagcactt gaggaaaccc caaagggcat gcctaaagac ttgaatgtgg gtcagcaagc 2521 tctttccaac agtggcatct cagaggttga gggtctctct caacatcagg tgcctgaatt 2581 gagctgtacg tcagctgctg atgccatcaa caggaactca acaggccagc aaagccaaag 2641 cggttcacct tgcattatgg atgacacagc atttaataga ggagtgaatg ccttcccgga 2701 ggctaaacat aaagcaggca ctgtgtgccc cactgtgacc cattccgttc attcccaggt 2761 tttgaaaaac caagagctga aggcaggcac ttccatcatg gggtcccatc tgacttctgc 2821 agagaccttt actctggaca gcctgaaggc tgtggaggtt gtgaacttat ctgtgtcttg 2881 cactgccacc tgtctccctt tctcatctgt gcccaaggag acccctgcca gggctggatt 2941 ctcttccaaa cagaccctgt ttcccatcac ccatcagcct ttgggatcct ttggagttgt 3001 ttctacccat tccagcacgt tggatgagga agtcagtgag aggatgttta gtttttatca 3061 ggccaaagaa aaatttaaaa aagaactgaa gattgaagga tttctgtaca gtgacttaac 3121 tgtactagct tctgatatac catatttccc accagaggaa gaggaagaaa atttggaaga 3181 tggaattcac ctggttgtct gtgtccatgg cctggatggg aacagtgcag acctccggct 3241 ggtaaagact ttcatagaac tggggctccc tggaggaaaa ctggacttcc taatgtctga 3301 aaagaatcag atggacacat ttgcagattt tgatactatg acggatcggt tattggatga 3361 aatcattcaa cacattcagt tgtacaacct ctccatatcc cgaattagct tcattggcca 3421 ttctcttggc aacatcatca tccgatcggt cctcacacgg ccccggttcc ggtattacct 3481 caacaaactc cacacgttcc tgtcactctc tgggcctcac ctgggaaccc tgtacaacaa 3541 cagcaccctg gttagtacag gcttatggcT CATGCAGAAA CTGAAGAAAT CCGGGTCTCT 3601 ACTGCAGCTG ACCTTCAGGG ATAATGCTGA TTTGCGCAAA TGTTTCCTCT ACCAACTAAG 3661 CCAAAAAACA GGGCTGCAGT ATTTTAAAAA CGTCGTGCTG GTTGCTTCTC CCCAAGACCG 3721 TTATGTGCCA TTTCATTCAG CCAGGATTGA AATGTGTAAA ACTGCCCTTA AAGACAGACA 3781 CACAGGGCCA GTTTATGCAG AAATGATCAA CAACCTCCTG GGCCCTCTGG TTGAAGCCAA 3841 GGACTGCACT TTAATCCGAC ACAACGTGTT CCACGCCCTG CCCAACACTG CCAACACCCT 3901 GATCGGCCGA GCCGCTCACA TCGCTGTGCT GGATTCAGAA CTCTTCCTGG AGAAGTTTTT 3961 CTTGGTGGCA GGACTCAACT ACTTAAGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC 4021 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 4081 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTAGTTG 4141 TGGATACGCT AGTAAGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 4201 aaagatt