Construct: ORF TRCN0000479308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013387.1_s317c1
- Derived from:
- ccsbBroadEn_13879
- DNA Barcode:
- AGAAGCATTGCACCCCTCCGACGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TNC (3371)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3371 | TNC | tenascin C | XM_011518625.3 | 95.3% | 8.1% | (many diffs) |
| 2 | human | 3371 | TNC | tenascin C | XM_005251973.4 | 95% | 8.9% | (many diffs) |
| 3 | human | 3371 | TNC | tenascin C | XM_005251972.4 | 91.2% | 7.8% | (many diffs) |
| 4 | human | 3371 | TNC | tenascin C | XM_011518628.3 | 90.3% | 9.4% | (many diffs) |
| 5 | human | 3371 | TNC | tenascin C | XM_006717097.4 | 88.5% | 7.8% | (many diffs) |
| 6 | human | 3371 | TNC | tenascin C | XM_011518626.3 | 87.4% | 8.5% | (many diffs) |
| 7 | human | 3371 | TNC | tenascin C | NM_002160.4 | 87.4% | 7.4% | (many diffs) |
| 8 | human | 3371 | TNC | tenascin C | XM_011518629.3 | 87.1% | 9.4% | (many diffs) |
| 9 | human | 3371 | TNC | tenascin C | XM_006717098.4 | 86.8% | 8.1% | (many diffs) |
| 10 | human | 3371 | TNC | tenascin C | XM_006717101.4 | 85.6% | 9.9% | (many diffs) |
| 11 | human | 3371 | TNC | tenascin C | XM_005251974.4 | 84.8% | 9.9% | (many diffs) |
| 12 | human | 3371 | TNC | tenascin C | XM_017014681.2 | 84.5% | 8.1% | (many diffs) |
| 13 | human | 3371 | TNC | tenascin C | XM_017014680.2 | 83.9% | 7.1% | (many diffs) |
| 14 | human | 3371 | TNC | tenascin C | XM_006717096.4 | 83.9% | 7.1% | (many diffs) |
| 15 | human | 3371 | TNC | tenascin C | XM_017014679.2 | 82.1% | 7.1% | (many diffs) |
| 16 | human | 3371 | TNC | tenascin C | XM_005251975.4 | 80.9% | 10.5% | (many diffs) |
| 17 | human | 3371 | TNC | tenascin C | XM_017014678.2 | 80.7% | 6.9% | (many diffs) |
| 18 | human | 3371 | TNC | tenascin C | XM_024447530.1 | 80.7% | 6.9% | (many diffs) |
| 19 | mouse | 21923 | Tnc | tenascin C | XM_006537777.1 | 69.1% | 9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 576
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggccatg actcagctgt tggcaggtgt ctttcttgct ttccttgccc 121 tcgctaccga aggtggggtc ctcaagaaag tcatccggca caagcgacag agtggggtga 181 acgccaccct gccagaagag aaccagccag tggtgtttaa ccacgtttac aacatcaagc 241 tgccagtggg atcccagtgt tcggtggatc tggagtcagc cagtggggag aaagacctgg 301 caccgccttc agagcccagc gaaagctttc aggagcacac agtggatggg gaaaaccaga 361 ttgtcttcac acatcgcatc aacatccccc gccgggcctg tggctgtgcc gcagcccctg 421 atgttaagga gctgctgagc agactggagg agctggagaa cctggtgtct tccctgaggg 481 agcaatgtac tgcaggagca ggctgctgtc tccagcctgc cacaggccgc ttggacacca 541 ggcccttctg tagcggtcgg ggcagacttc agcactgaag gatgtggctg tgtctgcgaa 601 cctggctgga aaggccccaa ctgctctgag cccgaatgtc caggcaactg tcaccttcga 661 ggccggtgca ttgatgggca gtgcatctgt gacgacggct tcacgggcga ggactgcagc 721 cagctggctt gccccagcga ctgcaatgac cagggcaagt gcgtaaatgg agtctgcatc 781 tgtttcgaag gctacgccgg ggctgactgc agccgtgaaa tctgcccagt gccctgcagt 841 gaggagcacg gcacatgtgt agatggcttg tgtgtgtgcc acgatggctt tgcaggcgat 901 gactgcaaca agcctctgtg tctcaacaat tgctacaacc gtggacgatg cgtggagaat 961 gagtgcgtgt gtgatgaggg tttcacgggc gaagactgca gtgagctcat ctgccccaat 1021 gactgcttcg accggggccg ctgcatcaat ggcacctgct actgcgaagg cttcacaggt 1081 gaagactgcg ggaaacccac ctgcccacat gcctgccaca cccagggccg gtgtgaggag 1141 gggcagtgtg tatgtgatga gggctttgcc ggtgtggact gcagcgagaa gaggtgtcct 1201 gctgactgtc acaatcgtgg ccgctgtgta gacgggcggt gtgagtgtga tgatggtttc 1261 actggagctg actgtgggga gctcaagtgt cccaatggct gcagtggcca tggccgctgt 1321 gtcaatgggc agtgtgtgtg tgatgagggc tatactgggg aggactgcag ccagctacgg 1381 tgccccaatg actgtcacag tcggggccgc tgtgtcgagg gcaaatgtgt atgtgagcaa 1441 ggcttcaagg gctatgactg cagtgacatg agctgcccta atgactgtca ccagcacggc 1501 cgctgtgtga atggcatgtg tgtttgtgat gacggctaca caggggaaga ctgccgggat 1561 cgccaatgcc ccagggactg cagcaacagg ggcctctgtg tggacggaca gtgcgtctgt 1621 gaggacggct tcaccggccc tgactgtgca gaactctcct gtccaaatga ctgccatggc 1681 cggggtcgct gtgtgaatgg gcagtgcgtg tgccatgaag gatttatggg caaagactgc 1741 aaggggcaaa gatgtcccag tgactgtcat ggccagggcc gctgcgtgga cggccagtgc 1801 atctgccacg agggcttcac aggcctggac tgtggccagc actcctgccc cagtgactgc 1861 aacaacttag gacaatgcgt ctcgggccgc tgcatctgca acgagggcta cagcggagaa 1921 gactgctcag aggtgtctcc tcccaaagac ctcgttgtga cagaagtgac ggaagagacg 1981 gtcaacctgg cctgggacaa tgagatgcgg gtcacagagt accttgtcgt gtacacgccc 2041 acccacgagg gtggtctgga aatgcagttc cgtgtgcctg gggaccagac gtccaccatc 2101 atccaggagc tggaacctgg tgtggagtac tttatccgtg tatttgccat cctggagaac 2161 aagaagagca ttcctgtcag cgccagggtg gccacgtact tacctgcacc tgaaggcctg 2221 aaattcaagt ccatcaagga gacatctgtg gaagtggagt gggatcctct agacattgct 2281 tttgaaacct gggagatcat cttccggaat atgaataaag aagatgaggg agagatcacc 2341 aaaagcctga ggaggccaga gacctcttac cggcaaactg gtctagctcc tgggcaagag 2401 tatgagatat ctctgcacat agtgaaaaac aatacccggg gccctggcct gaagagggtg 2461 accaccacac gcttggatgc ccccagccag atcgaggtga aagatgtcac agacaccact 2521 gccttgatca cctggttcaa gcccctggct gagatcgatg gcattgagct gacctacggc 2581 atcaaagacg tgccaggaga ccgtaccacc atcgatctca cagaggacga gaaccagtac 2641 tccatcggga acctgaagcc tgacactgag tacgaggtgt ccctcatctc ccgcagaggt 2701 gacatgtcaa gcaacccagc caaagagacc ttcacaacag gcctcgatgc tcccaggaat 2761 cttcgacgtg tttcccagac agataacagc atcaccctgg aatggaggaa tggcaaggca 2821 gctattgaca gttacagaat taagtatgcc cccatctctg gaggggacca cgctgaggtt 2881 gatgttccaa agagccaaca agccacaacc aaaaccacac tcacaggtct gaggccggga 2941 actgaatatg ggattggagt ttctgctgtg aaggaagaca aggagagcaa tccagcgacc 3001 atcaacgcag ccacagagtt ggacacgccc aaggaccttc aggtttctga aactgcagag 3061 accagcctga ccctgctctg gaagacaccg ttggccaaat ttgaccgcta ccgcctcaat 3121 tacagtctcc ccacaggcca gtgggtggga gtgcagcttc caagaaacac cacttcctat 3181 gtcctgagag gcctggaacc aggacaggag tacaatgtcc tcctgacagc cgagaaaggc 3241 agacacaaga gcaagcccgc acatgtgaag gcatccactg aacaagcccc tgagctggaa 3301 aacctcaccg tgactgaggt tggctgggat ggcctcagac tcaactggac cgcagctgac 3361 caggcctatg agcactttat cattcaggtg caggaggcca acaaggtgga ggcagctcgg 3421 aacctcaccg tgcctggcag ccttcgggct gtggacatac cgggcctcaa ggctgctacg 3481 ccttatacag tctccatcta tggggtgatc cagggctata gaacaccagt gctctctgct 3541 gaggcctcca caggggaaac tcccaatttg ggagaggtcg tggtggccga ggtgggctgg 3601 gatgccctca aactcaactg gactgctcca gaaggggcct atgagtactt tttcattcag 3661 gtgcaggagg ctgacacagt agaggcagcc cagaacctca ccgtcccagg aggactgggg 3721 tccacagacc tgcctgggct caaagcagcc actcattata ccatcaccat ccgcggggtc 3781 actcaggact tcagcacaac ccctctctct gttggagtct tgacagagga ggttccagat 3841 atgggaaacc tcacagtgac cgaggttagc tgggatgctc tcagactgaa ctggaccacg 3901 ccagatggaa cctatgacca gtttactatt caggtccagg aggctgacca ggtggaagag 3961 gctcacaatc tcacggttcc tggcagcctg cgttccatgg aaatcccagg cctcagggct 4021 ggcactcctt acacagtcac cctgcacggc gaggtcaggg gccacagcac tcgacccctt 4081 gctgtagagg tcgtcacaga ggatctccca cagctgggag atttagccgt gtctgaggtt 4141 ggctgggatg gcctcagact caactggacc gcagctgaca atgcctatga gcactttgtc 4201 attcaggtgc aggaggtcaa caaagtggag gcagcccaga acctcacgtt gcctggcagc 4261 ctcagggctg tggacatccc gggcctcgag gctgccacgc cttatagagt ctccatctat 4321 ggggtgatcc ggggctatag aacaccagta ctctctgctg aggcctccac agccatgggc 4381 tccccaaagg aagtcatttt ctcagacatc actgaaaatt cggctactgt cagctggagg 4441 gcacccacag cccaagtgga gagcttccgg attacctatg tgcccattac aggaggtaca 4501 ccctccatgg taactgtgga cggaaccaag actcagacca ggctggtgaa actcatacct 4561 ggcgtggagt accttgtcag catcatcgcc atgaagggct ttgaggaaag tgaacctgtc 4621 tcagggtcat tcaccacagc tctggatggc ccatctggcc tggtgacagc caacatcact 4681 gactcagaag ccttggccag gtggcagcca gccattgcca ctgtggacag ttatgtcatc 4741 tcctacacag gcgagaaagt gccagaaatt acacgcacgg tgtccgggaa cacagtggag 4801 tatgctctga ccgacctcga gcctgccacg gaatacacac tgagaatctt tgcagagaaa 4861 gggccccaga agagctcaac catcactgcc aagttcacaa cagacctcga ttctccaaga 4921 gacttgactg ctactgaggt tcagtcggaa actgccctcc ttacctggcg acccccccgg 4981 gcatcagtca ccggttacct gctggtctat gaatcagtgg atggcacagt caaggaagtc 5041 attgtgggtc cagataccac ctcctacagc ctggcagacc tgagcccatc cacccactac 5101 acagccaaga tccaggcact caatgggccc ctgaggagca atatgatcca gaccatcttc 5161 accacaattg gactcctgta ccccttcccc aaggactgct cccaagcaat gctgaatgga 5221 gacacgacct ctggcctcta caccatttat ctgaatggtg ataaggctca ggcgctggaa 5281 gtcttctgtg acatgacctc tgatgggggt ggatggattg tgttcctgag acgcaaaaac 5341 ggacgcgaga acttctacca aaactggaag gcatatgctg ctggatttgg ggaccgcaga 5401 gaagaattct ggcttgggct ggacaacctg aacaaaatca cagcccaggg gcagtacgag 5461 ctccGGGTGG ACCTGCGGGA CCATGGGGAG ACAGCCTTTG CTGTCTATGA CAAGTTCAGC 5521 GTGGGAGATG CCAAGACTCG CTACAAGCTG AAGGTGGAGG GGTACAGTGG GACAGCAGGT 5581 GACTCCATGG CCTACCACAA TGGCAGATCC TTCTCCACCT TTGACAAGGA CACAGATTCA 5641 GCCATCACCA ACTGTGCTCT GTCCTACAAA GGGGCTTTCT GGTACAGGAA CTGTCACCGT 5701 GTCAACCTGA TGGGGAGATA TGGGGACAAT AACCACAGTC AGGGCGTTAA CTGGTTCCAC 5761 TGGAAGGGCC ACGAACACTC AATCCAGTTT GCTGAGATGA AGCTGAGACC AAGCAACTTC 5821 AGAAATCTTG AAGGCAGGCG CAAACGGGCA TTGCCAACTT TCTTGTACAA AGTGGTTGAT 5881 ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT 5941 CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGAA 6001 GCATTGCACC CCTCCGACGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 6061 gtgaaagatt