Construct: ORF TRCN0000479313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014507.1_s317c1
- Derived from:
- ccsbBroadEn_09622
- DNA Barcode:
- CCTCCTGATATCTTGTGGACACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM161B (145483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145483 | FAM161B | FAM161 centrosomal protein B | NM_152445.3 | 100% | 100% | |
| 2 | human | 145483 | FAM161B | FAM161 centrosomal protein B | XM_011536475.2 | 89.2% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2010
- ORF length:
- 1941
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgtgggg aggcctgagg gagcccccgg aggcgcggag gggagccgtc 121 agatatttcc ccccgagtcc ttcgcagaca cagaggcagg agaggagctg tccggggatg 181 ggctggtttt gcccagggcc agcaaacttg acgagttcct cagcccagag gaggagatag 241 attctacttc tgactcaact gggagcattt accagaactt acaggaactg aagcagaaag 301 ggagatggtg tctgttggag tctctctttc agtctgaccc agagagtgat gaaaacctct 361 ctgaagatga ggaggacctg gagagtttct tccaagacaa ggacaggggg atggtgcagg 421 tccagtgccc gcaggctctg aggtgtggct ccacaaggcg ctgcagctcc ctgaacaacc 481 ttccctccaa cattcccagg cctcagaccc agccaccctc aggctcccgg cctccctccc 541 agcacagaag cgtcagctcc tgggcatcat ccattactgt ccctcggcca ttccgcatga 601 cgctgcgcga ggcccggaag aaggccgagt ggctgggctc acctgcctcc tttgagcagg 661 agaggcagcg ggcccagagg cagggtgagg aagaggccga gtgccacagg cagttccggg 721 cacagcctgt gcctgcacat gtctacctgc ccctctacca agagatcatg gagcgcagcg 781 aggcccgaag gcaggcaggg atccagaaga ggaaggaact gctcctctct tctttgaagc 841 ccttcagctt cctggagaag gaggagcagc taaaggaagc tgctcgacag agagacttgg 901 cagccacagc tgaagccaag atctccaagc agaaggccac cagaaggatt cccaagtcca 961 ttctggagcc agcccttggg gataaactcc aggaagctga gctcttcagg aaaattcgca 1021 tccaaatgag agccctggac atgctccaga tggcctcttc ccctatcgcc tcctctagta 1081 accgggctaa cccacagccc cgcacagcca cccgaaccca gcaggaaaag cttgggtttc 1141 tgcacactaa cttcagattc cagcctcggg tgaatcctgt ggtccctgac tatgagggcc 1201 tttacaaggc cttccagaga agagcagcca aaagaagaga aacccaagag gccactcgca 1261 acaagccctt cttgctgagg accgccaacc tgcgccaccc tcagcggccc tgtgatgctg 1321 ccaccaccgg aaggaggcag gattccccac agccaccagc tacacccctg ccaaggagtc 1381 gttctctgag cggccttgct tccctctctg ccaacactct ccctgtgcac atcacagatg 1441 ccaccaggaa gagggaatct gcagtcagaa gtgcacttga aaaaaagaac aaagcagatg 1501 agagtattca gtggctggag atacacaaaa agaagtctca agcaatgtcc aaatctgtga 1561 ccTTGCGTGC AAAAGCCATG GATCCCCATA AAAGCCTGGA GGAAGTGTTC AAAGCAAAGC 1621 TGAAAGAGAA CCGGAACAAT GACCGTAAAA GAGCGAAAGA ATATAAGAAA GAACTGGAGG 1681 AAATGAAGCA GCGAATACAA ACAAGGCCCT ATCTCTTTGA ACAAGTTGCC AAGGATCTAG 1741 CCAAGAAAGA AGCAGAACAG TGGTATCTAG ACACCCTGAA GCAGGCTGGG CTGGAGGAAG 1801 ACTTTGTGAG AAACAAGGGT CAAGGCACCC GGGCTGTTCA AGAGAAAGAG ACCAAAATCA 1861 AGGATTTTCC CAGGTTCCAA GAAACTACAA AACTCAGCAT CAGAGATCCA GAGCAGGGTT 1921 TAGAAGGATC TCTAGAACAG CCTGCAAGCC CCAGGAAAGT ACTGGAGGAG CTGTCTCATC 1981 AGTCACCAGA AAATCTCGTA TCACTTGCTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2041 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2101 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCTCC 2161 TGATATCTTG TGGACACATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2221 tgaaagatt