Construct: ORF TRCN0000479313

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014507.1_s317c1
Derived from:
ccsbBroadEn_09622
DNA Barcode:
CCTCCTGATATCTTGTGGACACAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FAM161B (145483)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479313
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 145483 FAM161B FAM161 centrosomal protein B NM_152445.3 100% 100%
2 human 145483 FAM161B FAM161 centrosomal protein B XM_011536475.2 89.2% 88.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2010
ORF length:
1941
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaccgtgggg aggcctgagg gagcccccgg aggcgcggag gggagccgtc
121agatatttcc ccccgagtcc ttcgcagaca cagaggcagg agaggagctg tccggggatg
181ggctggtttt gcccagggcc agcaaacttg acgagttcct cagcccagag gaggagatag
241attctacttc tgactcaact gggagcattt accagaactt acaggaactg aagcagaaag
301ggagatggtg tctgttggag tctctctttc agtctgaccc agagagtgat gaaaacctct
361ctgaagatga ggaggacctg gagagtttct tccaagacaa ggacaggggg atggtgcagg
421tccagtgccc gcaggctctg aggtgtggct ccacaaggcg ctgcagctcc ctgaacaacc
481ttccctccaa cattcccagg cctcagaccc agccaccctc aggctcccgg cctccctccc
541agcacagaag cgtcagctcc tgggcatcat ccattactgt ccctcggcca ttccgcatga
601cgctgcgcga ggcccggaag aaggccgagt ggctgggctc acctgcctcc tttgagcagg
661agaggcagcg ggcccagagg cagggtgagg aagaggccga gtgccacagg cagttccggg
721cacagcctgt gcctgcacat gtctacctgc ccctctacca agagatcatg gagcgcagcg
781aggcccgaag gcaggcaggg atccagaaga ggaaggaact gctcctctct tctttgaagc
841ccttcagctt cctggagaag gaggagcagc taaaggaagc tgctcgacag agagacttgg
901cagccacagc tgaagccaag atctccaagc agaaggccac cagaaggatt cccaagtcca
961ttctggagcc agcccttggg gataaactcc aggaagctga gctcttcagg aaaattcgca
1021tccaaatgag agccctggac atgctccaga tggcctcttc ccctatcgcc tcctctagta
1081accgggctaa cccacagccc cgcacagcca cccgaaccca gcaggaaaag cttgggtttc
1141tgcacactaa cttcagattc cagcctcggg tgaatcctgt ggtccctgac tatgagggcc
1201tttacaaggc cttccagaga agagcagcca aaagaagaga aacccaagag gccactcgca
1261acaagccctt cttgctgagg accgccaacc tgcgccaccc tcagcggccc tgtgatgctg
1321ccaccaccgg aaggaggcag gattccccac agccaccagc tacacccctg ccaaggagtc
1381gttctctgag cggccttgct tccctctctg ccaacactct ccctgtgcac atcacagatg
1441ccaccaggaa gagggaatct gcagtcagaa gtgcacttga aaaaaagaac aaagcagatg
1501agagtattca gtggctggag atacacaaaa agaagtctca agcaatgtcc aaatctgtga
1561ccTTGCGTGC AAAAGCCATG GATCCCCATA AAAGCCTGGA GGAAGTGTTC AAAGCAAAGC
1621TGAAAGAGAA CCGGAACAAT GACCGTAAAA GAGCGAAAGA ATATAAGAAA GAACTGGAGG
1681AAATGAAGCA GCGAATACAA ACAAGGCCCT ATCTCTTTGA ACAAGTTGCC AAGGATCTAG
1741CCAAGAAAGA AGCAGAACAG TGGTATCTAG ACACCCTGAA GCAGGCTGGG CTGGAGGAAG
1801ACTTTGTGAG AAACAAGGGT CAAGGCACCC GGGCTGTTCA AGAGAAAGAG ACCAAAATCA
1861AGGATTTTCC CAGGTTCCAA GAAACTACAA AACTCAGCAT CAGAGATCCA GAGCAGGGTT
1921TAGAAGGATC TCTAGAACAG CCTGCAAGCC CCAGGAAAGT ACTGGAGGAG CTGTCTCATC
1981AGTCACCAGA AAATCTCGTA TCACTTGCTT TGCCAACTTT CTTGTACAAA GTGGTTGATA
2041TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2101CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCTCC
2161TGATATCTTG TGGACACATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2221tgaaagatt

Download FASTA (ORF) (Full)