Construct: ORF TRCN0000479319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002916.1_s317c1
- Derived from:
- ccsbBroadEn_14077
- DNA Barcode:
- TGAAGCCACGTGCATCACTTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTC28 (23331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23331 | TTC28 | tetratricopeptide repeat do... | XM_006724171.4 | 53% | 53% | 1_3327del |
| 2 | human | 23331 | TTC28 | tetratricopeptide repeat do... | XM_017028673.2 | 51.1% | 51.1% | 1_3591del |
| 3 | human | 23331 | TTC28 | tetratricopeptide repeat do... | XM_011530018.3 | 51% | 51% | 1_3603del |
| 4 | human | 23331 | TTC28 | tetratricopeptide repeat do... | NM_001145418.1 | 50.5% | 50.5% | 1_3681del |
| 5 | human | 23331 | TTC28 | tetratricopeptide repeat do... | XM_005261405.2 | 50.2% | 50.2% | 1_3681del;5552_5553ins24 |
| 6 | mouse | 209683 | Ttc28 | tetratricopeptide repeat do... | XM_006534846.3 | 42.7% | 44.5% | (many diffs) |
| 7 | mouse | 209683 | Ttc28 | tetratricopeptide repeat do... | XM_006534845.3 | 42.4% | 44.1% | (many diffs) |
| 8 | mouse | 209683 | Ttc28 | tetratricopeptide repeat do... | NM_001267622.1 | 40.8% | 42.4% | (many diffs) |
| 9 | mouse | 209683 | Ttc28 | tetratricopeptide repeat do... | XM_006534844.3 | 40.5% | 42.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3828
- ORF length:
- 3762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt aaatggccag aggggactag tgctttacta ttccctggct gcaggctatc 121 tgtatagctg gctgctggct cctggggcag gaattgtgaa gtttcatgaa cactacctgg 181 gtgagaacac agtggaaaac tcaagtgact tccaggccag cagcagtgta acccttccaa 241 cagcaaccgg ctcagccctg gagcagcaca ttgccagtgt ccgggaggcc ctgggggtgg 301 agtctcacta ctcaagggcc tgtgccagca gtgagacaga gagtgaagcg ggagacatca 361 tggaccagca atttgaagag atgaacaaca aactcaactc ggtcactgac cccactggct 421 ttctgcggat ggttcgccgc aataacctgt ttaacaggag ctgccagagc atgacgagcc 481 tgttcagtaa cactgtgtca ccgacccagg acgggacctc ctctcttccc aggaggcaga 541 gctcgtttgc caagcccccg ctccgtgccc tgtatgacct gctcatcgcg cccatggaag 601 ggggcctgat gcactccagc ggccccgtgg gccggcaccg gcagctcatc ctggttctgg 661 agggggagct ctacctcatt cctttcgccc tcctgaaggg aagctcctcc aatgagtacc 721 tctacgagcg cttcggcctc cttgctgtcc cttccatccg ctccctcagc gtgcagtcca 781 agtctcactt acggaagaac ccgcccacat actccagctc cacatccatg gcggctgtca 841 tcggcaaccc caagctacca tcggccgtga tggacaggtg gctgtggggg cccatgccat 901 cggccgagga agaggcctac atggtgtccg agctgctggg ctgccagccc ctagtgggca 961 gtgtggccac caaggagagg gtcatgagtg ccctgaccca ggctgaatgc gtccactttg 1021 ccacccacat ctcctggaag ctgtcggcct tggtcctcac gcccagcatg gacggcaacc 1081 ctgccagcag caagagctcc ttcggccacc cctacacgat ccctgagtcc ttgcgggtgc 1141 aggacgatgc cagtgatggg gagagcatct cggactgccc gcccctgcag gagctgctgc 1201 ttactgccgc cgacgtcctg gacctgcagc tgcctgtgaa gctggtggtg cttggctcct 1261 cccaggagtc caacagcaaa gtcacagccg acggggtcat cgcgctgaca agggccttcc 1321 tggctgccgg cgctcagtgt gtcctcgtgt ctctgtggcc tgtgccagtg gctgcttcta 1381 agatgttcat ccatgccttc tactcatccc tgctgaacgg cctgaaagcc agcgccgccc 1441 tgggggaggc catgaaggtg gtgcagagca gcaaggcctt ctcgcacccc tccaactggg 1501 cagggttcat gctcatcggg agtgacgtta agctgaacag cccctcatca ctcatcggcc 1561 aggccctcac agagatcctg cagcacccgg agcgtgcgcg ggacgccctg cgagtgctgc 1621 tgcacctggt ggagaaatcc ctgcagcgca tccagaatgg gcagcgcaat gccatgtaca 1681 catcccagca gagtgtggag aacaaagtgg gcggcatccc tggctggcag gccctcctca 1741 ccgctgtggg cttccggctg gaccccccaa ccagtggcct gccagcggct gtcttcttcc 1801 caacctccga cccgggcgac cggctccagc agtgcagcag cacactccag tccctgctgg 1861 gtctgcccaa tcctgccctc caagcccttt gcaaactcat cactgcctcc gagacgggcg 1921 agcagctcat cagccgggct gttaaaaata tggttggaat gctccaccag gtgctggttc 1981 agctccaggc tggcgagaag gagcaggact tggcatcagc tcccattcag gtctccatca 2041 gcgtccagct gtggcggctc ccgggatgcc acgagttcct ggcagctcta ggttttgatc 2101 tctgtgaagt tggtcaggag gaagtaatcc tgaaaaccgg gaagcaagct aatcgacgga 2161 ctgtgcactt cgcgctccag tccctgctgt ctctgtttga ttctactgag ctacccaagc 2221 gcctcagcct tgacagctcc tcctccctcg agtctcttgc ttctgctcag tctgtttcca 2281 acgccctgcc cttgggttac cagcaacccc ccttctctcc caccggtgcg gacagcatcg 2341 cctcagatgc catctctgtg tacagtctga gctccattgc ctcctcaatg agctttgtct 2401 ccaaacccga gggtggatca gagggtggag gccccggagg acggcaggac catgaccggt 2461 ccaagaacgc ttacctgcag agatccaccc tgcctaggag ccagctgcct ccccagaccc 2521 gccctgcagg caacaaagat gaagaagaat atgaagggtt ttctatcatc agtaacgagc 2581 ccttggcgac ctaccaagaa aaccgaaaca catgcttctc accagaccac aaacaacccc 2641 aacctgggac agccggaggc atgagagtct cggtgagctc caaagggagc atcagcactc 2701 caaattctcc agtgaaaatg actctgattc ccagccccaa ctcacccttc caaaaggtgg 2761 gaaaactagc aagctcagat acaggagaat cagaccagtc tagcacagaa acggacagta 2821 ccgtgaaatc ccaagaagaa agcaacccaa aactggatcc acaagagtta gcccagaaaa 2881 ttctggagga gacacagagt catctcattg cggtggagcg tcttcagagg agcggcggcc 2941 aggtgagcaa gagtaataac cctgaagacg gcgttcaggc gcccagcagc actgctgtct 3001 tcagagcgtc agaaaccagt gcgttcagca ggcctgttct ctcccatcag aagagtcagc 3061 catcaccagt cactgttaaa ccaaagcccc cagccaggag ctcctccctg cccaaggtga 3121 gttccggata tagcagcccc accacctcag agatgtccat caaagacagc ccgagccagc 3181 acagtggccg gccatcgccc ggctgcgact cacagacttc ccagctggac cagcctctct 3241 ttaaactgaa gtaccccagc tctccttaca gcgctcacat ttccaaatca ccaaggaaca 3301 tgtccccaag ctccggccac cagtctcctg ctggcagtgc accctcccca gctctctcct 3361 actcctcagc tGGATCTGCT CGCTCAAGTC CAGCAGACGC TCCCGACATA GACAAACTGA 3421 AAATGGCAGC CATTGATGAA AAGGTGCAGG CTGTCCATAA CCTGAAGATG TTCTGGCAGA 3481 GCACACCCCA GCATTCCACA GGGCCAATGA AGATCTTCCG GGGGGCTCCT GGCACGATGA 3541 CTTCCAAAAG GGATGTCCTC AGTCTGTTGA ATTTGTCACC ACGGCACAAT AAGAAGGAGG 3601 AGGGAGTGGA TAAGCTTGAA CTGAAGGAGC TGTCCCTGCA GCAGCATGAC GGAGCTCCAC 3661 CGAAAGCCCC TCCCAACGGA CACTGGCGCA CCGAGACCAC CTCGCTGGGC TCACTGCCGC 3721 TGCCCGCCGG CCCTCCCGCC ACAGCCCCCG CGCGCCCTTT GAGGCTTCCT TCTGGAAATG 3781 GCTACAAGTT CCTGTCTCCA GGAAGATTTT TCCCTTCTTC CAAATGCTAC CCAACTTTCT 3841 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3901 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3961 GTGGAAAGGA CGATGAAGCC ACGTGCATCA CTTGCCAACG CGTTAAGTCg acaatcaacc 4021 tctggattac aaaatttgtg aaagatt