Construct: ORF TRCN0000479319

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002916.1_s317c1
Derived from:
ccsbBroadEn_14077
DNA Barcode:
TGAAGCCACGTGCATCACTTGCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TTC28 (23331)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479319
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23331 TTC28 tetratricopeptide repeat do... XM_006724171.4 53% 53% 1_3327del
2 human 23331 TTC28 tetratricopeptide repeat do... XM_017028673.2 51.1% 51.1% 1_3591del
3 human 23331 TTC28 tetratricopeptide repeat do... XM_011530018.3 51% 51% 1_3603del
4 human 23331 TTC28 tetratricopeptide repeat do... NM_001145418.1 50.5% 50.5% 1_3681del
5 human 23331 TTC28 tetratricopeptide repeat do... XM_005261405.2 50.2% 50.2% 1_3681del;5552_5553ins24
6 mouse 209683 Ttc28 tetratricopeptide repeat do... XM_006534846.3 42.7% 44.5% (many diffs)
7 mouse 209683 Ttc28 tetratricopeptide repeat do... XM_006534845.3 42.4% 44.1% (many diffs)
8 mouse 209683 Ttc28 tetratricopeptide repeat do... NM_001267622.1 40.8% 42.4% (many diffs)
9 mouse 209683 Ttc28 tetratricopeptide repeat do... XM_006534844.3 40.5% 42.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3828
ORF length:
3762
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt aaatggccag aggggactag tgctttacta ttccctggct gcaggctatc
121tgtatagctg gctgctggct cctggggcag gaattgtgaa gtttcatgaa cactacctgg
181gtgagaacac agtggaaaac tcaagtgact tccaggccag cagcagtgta acccttccaa
241cagcaaccgg ctcagccctg gagcagcaca ttgccagtgt ccgggaggcc ctgggggtgg
301agtctcacta ctcaagggcc tgtgccagca gtgagacaga gagtgaagcg ggagacatca
361tggaccagca atttgaagag atgaacaaca aactcaactc ggtcactgac cccactggct
421ttctgcggat ggttcgccgc aataacctgt ttaacaggag ctgccagagc atgacgagcc
481tgttcagtaa cactgtgtca ccgacccagg acgggacctc ctctcttccc aggaggcaga
541gctcgtttgc caagcccccg ctccgtgccc tgtatgacct gctcatcgcg cccatggaag
601ggggcctgat gcactccagc ggccccgtgg gccggcaccg gcagctcatc ctggttctgg
661agggggagct ctacctcatt cctttcgccc tcctgaaggg aagctcctcc aatgagtacc
721tctacgagcg cttcggcctc cttgctgtcc cttccatccg ctccctcagc gtgcagtcca
781agtctcactt acggaagaac ccgcccacat actccagctc cacatccatg gcggctgtca
841tcggcaaccc caagctacca tcggccgtga tggacaggtg gctgtggggg cccatgccat
901cggccgagga agaggcctac atggtgtccg agctgctggg ctgccagccc ctagtgggca
961gtgtggccac caaggagagg gtcatgagtg ccctgaccca ggctgaatgc gtccactttg
1021ccacccacat ctcctggaag ctgtcggcct tggtcctcac gcccagcatg gacggcaacc
1081ctgccagcag caagagctcc ttcggccacc cctacacgat ccctgagtcc ttgcgggtgc
1141aggacgatgc cagtgatggg gagagcatct cggactgccc gcccctgcag gagctgctgc
1201ttactgccgc cgacgtcctg gacctgcagc tgcctgtgaa gctggtggtg cttggctcct
1261cccaggagtc caacagcaaa gtcacagccg acggggtcat cgcgctgaca agggccttcc
1321tggctgccgg cgctcagtgt gtcctcgtgt ctctgtggcc tgtgccagtg gctgcttcta
1381agatgttcat ccatgccttc tactcatccc tgctgaacgg cctgaaagcc agcgccgccc
1441tgggggaggc catgaaggtg gtgcagagca gcaaggcctt ctcgcacccc tccaactggg
1501cagggttcat gctcatcggg agtgacgtta agctgaacag cccctcatca ctcatcggcc
1561aggccctcac agagatcctg cagcacccgg agcgtgcgcg ggacgccctg cgagtgctgc
1621tgcacctggt ggagaaatcc ctgcagcgca tccagaatgg gcagcgcaat gccatgtaca
1681catcccagca gagtgtggag aacaaagtgg gcggcatccc tggctggcag gccctcctca
1741ccgctgtggg cttccggctg gaccccccaa ccagtggcct gccagcggct gtcttcttcc
1801caacctccga cccgggcgac cggctccagc agtgcagcag cacactccag tccctgctgg
1861gtctgcccaa tcctgccctc caagcccttt gcaaactcat cactgcctcc gagacgggcg
1921agcagctcat cagccgggct gttaaaaata tggttggaat gctccaccag gtgctggttc
1981agctccaggc tggcgagaag gagcaggact tggcatcagc tcccattcag gtctccatca
2041gcgtccagct gtggcggctc ccgggatgcc acgagttcct ggcagctcta ggttttgatc
2101tctgtgaagt tggtcaggag gaagtaatcc tgaaaaccgg gaagcaagct aatcgacgga
2161ctgtgcactt cgcgctccag tccctgctgt ctctgtttga ttctactgag ctacccaagc
2221gcctcagcct tgacagctcc tcctccctcg agtctcttgc ttctgctcag tctgtttcca
2281acgccctgcc cttgggttac cagcaacccc ccttctctcc caccggtgcg gacagcatcg
2341cctcagatgc catctctgtg tacagtctga gctccattgc ctcctcaatg agctttgtct
2401ccaaacccga gggtggatca gagggtggag gccccggagg acggcaggac catgaccggt
2461ccaagaacgc ttacctgcag agatccaccc tgcctaggag ccagctgcct ccccagaccc
2521gccctgcagg caacaaagat gaagaagaat atgaagggtt ttctatcatc agtaacgagc
2581ccttggcgac ctaccaagaa aaccgaaaca catgcttctc accagaccac aaacaacccc
2641aacctgggac agccggaggc atgagagtct cggtgagctc caaagggagc atcagcactc
2701caaattctcc agtgaaaatg actctgattc ccagccccaa ctcacccttc caaaaggtgg
2761gaaaactagc aagctcagat acaggagaat cagaccagtc tagcacagaa acggacagta
2821ccgtgaaatc ccaagaagaa agcaacccaa aactggatcc acaagagtta gcccagaaaa
2881ttctggagga gacacagagt catctcattg cggtggagcg tcttcagagg agcggcggcc
2941aggtgagcaa gagtaataac cctgaagacg gcgttcaggc gcccagcagc actgctgtct
3001tcagagcgtc agaaaccagt gcgttcagca ggcctgttct ctcccatcag aagagtcagc
3061catcaccagt cactgttaaa ccaaagcccc cagccaggag ctcctccctg cccaaggtga
3121gttccggata tagcagcccc accacctcag agatgtccat caaagacagc ccgagccagc
3181acagtggccg gccatcgccc ggctgcgact cacagacttc ccagctggac cagcctctct
3241ttaaactgaa gtaccccagc tctccttaca gcgctcacat ttccaaatca ccaaggaaca
3301tgtccccaag ctccggccac cagtctcctg ctggcagtgc accctcccca gctctctcct
3361actcctcagc tGGATCTGCT CGCTCAAGTC CAGCAGACGC TCCCGACATA GACAAACTGA
3421AAATGGCAGC CATTGATGAA AAGGTGCAGG CTGTCCATAA CCTGAAGATG TTCTGGCAGA
3481GCACACCCCA GCATTCCACA GGGCCAATGA AGATCTTCCG GGGGGCTCCT GGCACGATGA
3541CTTCCAAAAG GGATGTCCTC AGTCTGTTGA ATTTGTCACC ACGGCACAAT AAGAAGGAGG
3601AGGGAGTGGA TAAGCTTGAA CTGAAGGAGC TGTCCCTGCA GCAGCATGAC GGAGCTCCAC
3661CGAAAGCCCC TCCCAACGGA CACTGGCGCA CCGAGACCAC CTCGCTGGGC TCACTGCCGC
3721TGCCCGCCGG CCCTCCCGCC ACAGCCCCCG CGCGCCCTTT GAGGCTTCCT TCTGGAAATG
3781GCTACAAGTT CCTGTCTCCA GGAAGATTTT TCCCTTCTTC CAAATGCTAC CCAACTTTCT
3841TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
3901CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
3961GTGGAAAGGA CGATGAAGCC ACGTGCATCA CTTGCCAACG CGTTAAGTCg acaatcaacc
4021tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)