Construct: ORF TRCN0000479379
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015116.2_s317c1
- Derived from:
- ccsbBroadEn_12056
- DNA Barcode:
- GTGGGCATATAACACATGTAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RC3H2 (54542)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479379
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54542 | RC3H2 | ring finger and CCCH-type d... | NM_001354486.2 | 99.8% | 99.8% | 417C>T;1502T>A |
| 2 | human | 54542 | RC3H2 | ring finger and CCCH-type d... | NM_018835.4 | 44.8% | 42.6% | (many diffs) |
| 3 | human | 54542 | RC3H2 | ring finger and CCCH-type d... | NM_001354478.2 | 42.2% | 40.1% | (many diffs) |
| 4 | human | 54542 | RC3H2 | ring finger and CCCH-type d... | NM_001354479.2 | 42% | 40% | (many diffs) |
| 5 | human | 54542 | RC3H2 | ring finger and CCCH-type d... | NM_001354482.2 | 41.3% | 39.3% | (many diffs) |
| 6 | human | 54542 | RC3H2 | ring finger and CCCH-type d... | NM_001100588.3 | 40% | 38% | (many diffs) |
| 7 | mouse | 319817 | Rc3h2 | ring finger and CCCH-type z... | NM_001290642.1 | 40.2% | 40.1% | (many diffs) |
| 8 | mouse | 319817 | Rc3h2 | ring finger and CCCH-type z... | NM_001100591.1 | 38.1% | 38% | (many diffs) |
| 9 | mouse | 319817 | Rc3h2 | ring finger and CCCH-type z... | XM_006498124.3 | 38.1% | 38% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1584
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgtgcaggca gctcaatgga cagaatttct gtcctgtcca atctgctata 121 atgaatttga tgagaatgtg cacaaaccca tcagtttagg ttgttcacac actgtttgca 181 agacctgctt gaataaactt catcgaaaag cttgtccttt tgaccagact gccatcaaca 241 cagatattga tgtacttcct gtcaacttcg cacttctcca gttagttgga gcccaggtac 301 cagatcatca gtcaattaag ttaagtaatc taggtgagaa taaacactat gaggttgcaa 361 agaaatgcgt tgaggatttg gcactctact taaaaccact aagtggaggt aaaggtgtag 421 ctagcttgaa ccagagtgca ctgagccgtc caatgcaaag gaaactggtg acacttgtaa 481 attgtcaact ggtggaggaa gaaggtcgtg taagagccat gcgagcagct cgttcccttg 541 gagaaagaac tgtaacagaa ctgatattac agcaccagaa ccctcagcag ttgtctgcca 601 atctatgggc cgctgtcagg gctcgaggat gccagttttt agggccagct atgcaagaag 661 aggccttgaa gctggtgtta ctggcattag aagatggttc tgccctctca aggaaagttc 721 tggtactttt tgttgtgcag agactagaac caagatttcc tcaggcatca aaaacaagta 781 ttggtcatgt tgtgcaacta ctgtatcgag cttcttgttt taaggttacc aaaagagatg 841 aagactcttc cctaatgcag ctgaaggagg aatttcggag ttatgaagca ttacgcagag 901 aacatgatgc ccaaattgtt catattgcca tggaagcagg actccgtatt tcacctgaac 961 agtggtcctc tcttttgtat ggtgatttgg ctcataaatc acacatgcag tctatcattg 1021 ataagctaca gtctccagag tcatttgcaa agagtgtcca ggaattgaca attgttttgc 1081 aacgaacagg tgacccagct aacttaaata gactgaggcc tcatttagag cttcttgcaa 1141 acatagaccc taatccagac gctgtttcac caacttggga gcagctggaa aatgcaatgg 1201 tagctgttaa aacagtagtt catggccttg tggacttcat acaaaattat agtagaaaag 1261 gccatgagac ccctcagccT CAGCCAAACA GCAAATACAA GACTAGCATG TGCCGAGATT 1321 TGCGACAGCA AGGGGGTTGT CCACGAGGAA CAAATTGTAC ATTTGCCCAT TCTCAGGAAG 1381 AGCTTGAAAA GTGTAACCCC CGTGGACTAT ATCTTCATTG TTGTCTCCTT TCCTCAGTGG 1441 CTAGCTTGGG CACTGTACAT AATGAGCTCT ACAAGCAGCA GCATTGTGAT AGAGAGAAGA 1501 TAAGTGTGTG TGCAGTGAAA AGAGCAGAAA CATGCTTCTC TTCTCAGTTT TTTTCCCCAC 1561 TTGAAGAGCC TAGAGAAGAG GATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1621 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1681 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TGGGCATATA 1741 ACACATGTAA GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1801 att