Construct: ORF TRCN0000479381
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007910.1_s317c1
- Derived from:
- ccsbBroadEn_00581
- DNA Barcode:
- CTAGAGCTACACCTGGAATCCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMO1 (2326)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479381
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2326 | FMO1 | flavin containing dimethyla... | NM_001282693.2 | 100% | 100% | |
| 2 | human | 2326 | FMO1 | flavin containing dimethyla... | NM_002021.3 | 100% | 100% | |
| 3 | human | 2326 | FMO1 | flavin containing dimethyla... | NM_001282692.1 | 99.2% | 99.2% | 1_12del |
| 4 | human | 2326 | FMO1 | flavin containing dimethyla... | XM_006711241.4 | 93.4% | 93.4% | 912_913ins105 |
| 5 | human | 2326 | FMO1 | flavin containing dimethyla... | NM_001282694.1 | 88.1% | 88.1% | 132_133ins189 |
| 6 | human | 2326 | FMO1 | flavin containing dimethyla... | XM_005245037.3 | 80.8% | 80.2% | (many diffs) |
| 7 | human | 2326 | FMO1 | flavin containing dimethyla... | XM_006711242.4 | 78.5% | 78.5% | 1254_1255ins342 |
| 8 | human | 2326 | FMO1 | flavin containing dimethyla... | XM_005245038.4 | 77.2% | 73.3% | 1182_1183ins73;1233_1234ins290 |
| 9 | mouse | 14261 | Fmo1 | flavin containing monooxyge... | NM_001330291.1 | 84.9% | 83.2% | (many diffs) |
| 10 | mouse | 14261 | Fmo1 | flavin containing monooxyge... | NM_010231.3 | 84.9% | 83.2% | (many diffs) |
| 11 | mouse | 14261 | Fmo1 | flavin containing monooxyge... | XM_006496663.3 | 84.9% | 83.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caagcgagtt gccattgtgg gagctggggt cagcggcctg gcctccatca 121 agtgctgtct ggaagaagga ctggagccca cctgctttga gaggagcgat gaccttgggg 181 ggctgtggag attcaccgaa catgttgaag aaggcagagc cagtctctac aagtctgtgg 241 tttccaacag ctgcaaggag atgtcttgtt actcagactt tccattccca gaagattatc 301 caaactatgt gccaaattct caattcctgg aatatctcaa aatgtatgca aaccactttg 361 accttctgaa acacattcaa ttcaagacca aagtctgcag tgtaacaaaa tgctcagatt 421 ctgctgtctc tggccaatgg gaggtggtca ctatgcatga agagaagcaa gagtcagcca 481 tctttgatgc tgtcatggtc tgcactggct ttcttactaa tccttatttg ccactggatt 541 cctttccagg tattaatgcc tttaaaggcc agtactttca tagccggcaa tataagcatc 601 cagatatatt taaggacaag agagtccttg tgattggaat gggaaattct ggcacagaca 661 ttgctgtgga ggccagccac ctggcggaaa aggtgttcct cagcaccacc ggagggggat 721 gggtgatcag ccgaatcttt gactcgggct acccatggga catggtgttc atgacacgct 781 ttcagaacat gttgagaaat tccctcccaa ccccaattgt gacttggttg atggagcgaa 841 agataaacaa ctggctcaat catgcaaatt acggcttaat accagaagac aggactcagc 901 tgaaagagtt tgtgctaaat gatgagctcc caggacgcat catcactggg aaagtgttca 961 tcaggccaag cataaaagag gtaaaggaaa actctgtcat atttaacaat acttcaaagg 1021 aagagcctat tgacatcatt gtctttgcca ctggatacac atttgctttc cccttccttg 1081 atgagtctgt agtgaaagtt gaagatggcc aggcctcact gtacaagtat atcttccctg 1141 cacatctgca aaagccaacc ctggccatta ttggcctcat caaacccttg ggctccatga 1201 tacctacagg agaaacacaa gctcggtggg ctgttcgagt cctgaaaggt gtaaataagt 1261 taccaccacc aagtgtcatg atagaggaaa ttaatgcaag gaaagaaaac aagcccagtt 1321 ggtttggctt gtgctactgc aaggctttac aatcagatta tatcacatac atagatgaac 1381 tcctgaccta tatcaatgca aaacccaacc tgttctctat gctcctaacg gatccacatc 1441 tggctctgac cgtcttcttt ggcccatgct caccatacca gttcCGCTTG ACTGGCCCAG 1501 GAAAATGGGA AGGAGCCAGA AATGCCATCA TGACCCAGTG GGACCGAACA TTCAAGGTCA 1561 TCAAAGCTCG AGTTGTACAA GAGTCTCCAT CTCCCTTTGA AAGTTTTCTT AAAGTCTTTA 1621 GCTTTCTGGC TTTGCTTGTG GCTATTTTTC TGATTTTCCT ATACCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 1801 GGACGACTAG AGCTACACCT GGAATCCAAT ACGCGTTAAG TCgacaatca acctctggat 1861 tacaaaattt gtgaaagatt